Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains

Autores
Gangoiti, María Virginia; Puertas, A. I.; Hamet, María Fernanda; Peruzzo, Pablo José; Llamas, M.G.; Medrano, Micaela; Prieto, A.; Dueñas, M.T.; Abraham, Analía Graciela
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium(SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces anEPS with a molecular weight of 104Da in both media. When grown in SDM produce an heteropolysac-charide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milkwas composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of thisEPS showed signals attributable to an a-glucan. Both by1H NMR and methylation analysis it was pos-sible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-D-glucopyranosil residues.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/119462
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grainsGangoiti, María VirginiaPuertas, A. I.Hamet, María FernandaPeruzzo, Pablo JoséLlamas, M.G.Medrano, MicaelaPrieto, A.Dueñas, M.T.Abraham, Analía GracielaQuímicaLactobacillus plantarumKefirExopolysaccharidesGlucansLactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium(SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces anEPS with a molecular weight of 104Da in both media. When grown in SDM produce an heteropolysac-charide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milkwas composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of thisEPS showed signals attributable to an a-glucan. Both by1H NMR and methylation analysis it was pos-sible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-D-glucopyranosil residues.Centro de Investigación y Desarrollo en Criotecnología de AlimentosInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf52-59http://sedici.unlp.edu.ar/handle/10915/119462enginfo:eu-repo/semantics/altIdentifier/issn/0144-8617info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2017.04.053info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:00:20Zoai:sedici.unlp.edu.ar:10915/119462Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:00:20.933SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
spellingShingle Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
Gangoiti, María Virginia
Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
title_short Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_full Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_fullStr Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_full_unstemmed Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
title_sort Lactobacillus plantarum CIDCA 8327: an α-glucan producing-strainisolated from kefir grains
dc.creator.none.fl_str_mv Gangoiti, María Virginia
Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
author Gangoiti, María Virginia
author_facet Gangoiti, María Virginia
Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
author_role author
author2 Puertas, A. I.
Hamet, María Fernanda
Peruzzo, Pablo José
Llamas, M.G.
Medrano, Micaela
Prieto, A.
Dueñas, M.T.
Abraham, Analía Graciela
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
topic Química
Lactobacillus plantarum
Kefir
Exopolysaccharides
Glucans
dc.description.none.fl_txt_mv Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium(SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces anEPS with a molecular weight of 104Da in both media. When grown in SDM produce an heteropolysac-charide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milkwas composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of thisEPS showed signals attributable to an a-glucan. Both by1H NMR and methylation analysis it was pos-sible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-D-glucopyranosil residues.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir withpromising properties for the development of functional foods. The aim of the present study was to char-acterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium(SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces anEPS with a molecular weight of 104Da in both media. When grown in SDM produce an heteropolysac-charide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milkwas composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of thisEPS showed signals attributable to an a-glucan. Both by1H NMR and methylation analysis it was pos-sible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-D-glucopyranosil residues.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/119462
url http://sedici.unlp.edu.ar/handle/10915/119462
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0144-8617
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2017.04.053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
52-59
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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