AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree

Autores
Somma, Stefania; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Mulè, Giuseppina; Fernández Pinto, Virginia Elena; Moretti, Antonio; Logrieco, Antonio Francesco
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease.
Fil: Somma, Stefania. Institute of Sciences of Food Production; Italia
Fil: Pose, Graciela Noemi. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mulè, Giuseppina. Institute of Sciences of Food Production; Italia
Fil: Fernández Pinto, Virginia Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moretti, Antonio. Institute of Sciences of Food Production; Italia
Fil: Logrieco, Antonio Francesco. Institute of Sciences of Food Production; Italia
Materia
A. ARBORESCENS
ALTERNARIA ALTERNATA/TENUISSIMA
ALTERNARIOL
MONOMETHYL ETHER
TENUAZONIC ACID
TOMATO BLACK MOULD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192764
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192764
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and pureeSomma, StefaniaPose, Graciela NoemiPardo, Alejandro GuillermoMulè, GiuseppinaFernández Pinto, Virginia ElenaMoretti, AntonioLogrieco, Antonio FrancescoA. ARBORESCENSALTERNARIA ALTERNATA/TENUISSIMAALTERNARIOLMONOMETHYL ETHERTENUAZONIC ACIDTOMATO BLACK MOULDhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease.Fil: Somma, Stefania. Institute of Sciences of Food Production; ItaliaFil: Pose, Graciela Noemi. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mulè, Giuseppina. Institute of Sciences of Food Production; ItaliaFil: Fernández Pinto, Virginia Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moretti, Antonio. Institute of Sciences of Food Production; ItaliaFil: Logrieco, Antonio Francesco. Institute of Sciences of Food Production; ItaliaElsevier Science2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192764Somma, Stefania; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Mulè, Giuseppina; Fernández Pinto, Virginia Elena; et al.; AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree; Elsevier Science; International Journal of Food Microbiology; 145; 2-3; 2-2011; 414-4190168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168160511000134info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2011.01.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:55Zoai:ri.conicet.gov.ar:11336/192764instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:55.913CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
title AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
spellingShingle AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
Somma, Stefania
A. ARBORESCENS
ALTERNARIA ALTERNATA/TENUISSIMA
ALTERNARIOL
MONOMETHYL ETHER
TENUAZONIC ACID
TOMATO BLACK MOULD
title_short AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
title_full AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
title_fullStr AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
title_full_unstemmed AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
title_sort AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
dc.creator.none.fl_str_mv Somma, Stefania
Pose, Graciela Noemi
Pardo, Alejandro Guillermo
Mulè, Giuseppina
Fernández Pinto, Virginia Elena
Moretti, Antonio
Logrieco, Antonio Francesco
author Somma, Stefania
author_facet Somma, Stefania
Pose, Graciela Noemi
Pardo, Alejandro Guillermo
Mulè, Giuseppina
Fernández Pinto, Virginia Elena
Moretti, Antonio
Logrieco, Antonio Francesco
author_role author
author2 Pose, Graciela Noemi
Pardo, Alejandro Guillermo
Mulè, Giuseppina
Fernández Pinto, Virginia Elena
Moretti, Antonio
Logrieco, Antonio Francesco
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv A. ARBORESCENS
ALTERNARIA ALTERNATA/TENUISSIMA
ALTERNARIOL
MONOMETHYL ETHER
TENUAZONIC ACID
TOMATO BLACK MOULD
topic A. ARBORESCENS
ALTERNARIA ALTERNATA/TENUISSIMA
ALTERNARIOL
MONOMETHYL ETHER
TENUAZONIC ACID
TOMATO BLACK MOULD
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease.
Fil: Somma, Stefania. Institute of Sciences of Food Production; Italia
Fil: Pose, Graciela Noemi. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mulè, Giuseppina. Institute of Sciences of Food Production; Italia
Fil: Fernández Pinto, Virginia Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moretti, Antonio. Institute of Sciences of Food Production; Italia
Fil: Logrieco, Antonio Francesco. Institute of Sciences of Food Production; Italia
description Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192764
Somma, Stefania; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Mulè, Giuseppina; Fernández Pinto, Virginia Elena; et al.; AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree; Elsevier Science; International Journal of Food Microbiology; 145; 2-3; 2-2011; 414-419
0168-1605
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192764
identifier_str_mv Somma, Stefania; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Mulè, Giuseppina; Fernández Pinto, Virginia Elena; et al.; AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree; Elsevier Science; International Journal of Food Microbiology; 145; 2-3; 2-2011; 414-419
0168-1605
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168160511000134
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2011.01.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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