Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transm...

Autores
Ruiz, María Julia; Salido, Jimena Patricia; Abusamra, Lorena; Ghiglione, Yanina Alexandra; Cevallos, Cintia Gisela; Damilano, Gabriel; Rodriguez, Ana María; Trifone, César Ariel; Laufer, Natalia Lorna; Giavedoni, Luis D.; Sued, Omar; Salomon, Horacio Eduardo; Gherardi, Maria Magdalena; Turk, Gabriela Julia Ana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.
Fil: Ruiz, María Julia.
Fil: Salido, Jimena.
Fil: Abusamra, Lorena.
Fil: Ghiglione, Yanina.
Fil: Cevallos, Cintia.
Fil: Damilano, Gabriel.
Fil: Rodriguez, Ana María.
Fil: Trifone, César Ariel. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Laufer, Natalia Lorna. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Giavedoni, Luis D.. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Sued, Omar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina
Fil: Salomón, Horacio.
Fil: Turk, Gabriela Julia Ana.
Materia
Adcc
Hiv-1
Hiv-1 Transmission
Serodiscordant Couples
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94312
Acceder
id CONICETDig_6c5fc5ff4e7769e9548a741c60252bac
oai_identifier_str oai:ri.conicet.gov.ar:11336/94312
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission RatesRuiz, María JuliaSalido, Jimena PatriciaAbusamra, LorenaGhiglione, Yanina AlexandraCevallos, Cintia GiselaDamilano, GabrielRodriguez, Ana MaríaTrifone, César ArielLaufer, Natalia LornaGiavedoni, Luis D.Sued, OmarSalomon, Horacio EduardoGherardi, Maria MagdalenaTurk, Gabriela Julia AnaAdccHiv-1Hiv-1 TransmissionSerodiscordant Coupleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.Fil: Ruiz, María Julia.Fil: Salido, Jimena.Fil: Abusamra, Lorena.Fil: Ghiglione, Yanina.Fil: Cevallos, Cintia.Fil: Damilano, Gabriel.Fil: Rodriguez, Ana María.Fil: Trifone, César Ariel. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: Laufer, Natalia Lorna. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: Giavedoni, Luis D.. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: Sued, Omar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; ArgentinaFil: Salomón, Horacio.Fil: Turk, Gabriela Julia Ana.Elsevier2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94312Ruiz, María Julia; Salido, Jimena Patricia; Abusamra, Lorena; Ghiglione, Yanina Alexandra; Cevallos, Cintia Gisela; et al.; Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates; Elsevier; EBioMedicine; 26; 12-2017; 25-372352-3964CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ebiom.2017.11.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352396417304401info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:47:09Zoai:ri.conicet.gov.ar:11336/94312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:47:10.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
title Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
spellingShingle Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
Ruiz, María Julia
Adcc
Hiv-1
Hiv-1 Transmission
Serodiscordant Couples
title_short Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
title_full Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
title_fullStr Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
title_full_unstemmed Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
title_sort Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
dc.creator.none.fl_str_mv Ruiz, María Julia
Salido, Jimena Patricia
Abusamra, Lorena
Ghiglione, Yanina Alexandra
Cevallos, Cintia Gisela
Damilano, Gabriel
Rodriguez, Ana María
Trifone, César Ariel
Laufer, Natalia Lorna
Giavedoni, Luis D.
Sued, Omar
Salomon, Horacio Eduardo
Gherardi, Maria Magdalena
Turk, Gabriela Julia Ana
author Ruiz, María Julia
author_facet Ruiz, María Julia
Salido, Jimena Patricia
Abusamra, Lorena
Ghiglione, Yanina Alexandra
Cevallos, Cintia Gisela
Damilano, Gabriel
Rodriguez, Ana María
Trifone, César Ariel
Laufer, Natalia Lorna
Giavedoni, Luis D.
Sued, Omar
Salomon, Horacio Eduardo
Gherardi, Maria Magdalena
Turk, Gabriela Julia Ana
author_role author
author2 Salido, Jimena Patricia
Abusamra, Lorena
Ghiglione, Yanina Alexandra
Cevallos, Cintia Gisela
Damilano, Gabriel
Rodriguez, Ana María
Trifone, César Ariel
Laufer, Natalia Lorna
Giavedoni, Luis D.
Sued, Omar
Salomon, Horacio Eduardo
Gherardi, Maria Magdalena
Turk, Gabriela Julia Ana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adcc
Hiv-1
Hiv-1 Transmission
Serodiscordant Couples
topic Adcc
Hiv-1
Hiv-1 Transmission
Serodiscordant Couples
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.
Fil: Ruiz, María Julia.
Fil: Salido, Jimena.
Fil: Abusamra, Lorena.
Fil: Ghiglione, Yanina.
Fil: Cevallos, Cintia.
Fil: Damilano, Gabriel.
Fil: Rodriguez, Ana María.
Fil: Trifone, César Ariel. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Laufer, Natalia Lorna. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Giavedoni, Luis D.. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Sued, Omar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina
Fil: Salomón, Horacio.
Fil: Turk, Gabriela Julia Ana.
description As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94312
Ruiz, María Julia; Salido, Jimena Patricia; Abusamra, Lorena; Ghiglione, Yanina Alexandra; Cevallos, Cintia Gisela; et al.; Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates; Elsevier; EBioMedicine; 26; 12-2017; 25-37
2352-3964
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94312
identifier_str_mv Ruiz, María Julia; Salido, Jimena Patricia; Abusamra, Lorena; Ghiglione, Yanina Alexandra; Cevallos, Cintia Gisela; et al.; Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates; Elsevier; EBioMedicine; 26; 12-2017; 25-37
2352-3964
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ebiom.2017.11.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352396417304401
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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