Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells

Autores
Su, Bin; Biedma, Marina Elizabeth; Lederle, Alexandre; Peressin, Maryse; Lambotin, Mélanie; Proust, Alizé; Decoville, Thomas; Schmidt, Sylvie; Laumond, Géraldine; Moog, Christiane
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We previously observed that HIV-1 replication in immature DCs is stimulated by cocultivation with primary T and B lymphocytes, suggesting that HIV-1 restriction in DCs may be overcome under coculture conditions. Here, we aimed to decipher the mechanism of SAMHD1-mediated restriction in DC-lymphocyte coculture. We found that coculture with lymphocytes downregulated SAMHD1 expression and was associated with increased HIV-1 replication in DCs. Moreover, in infected DC-T lymphocyte cocultures, DCs acquired maturation status and secreted type 1 interferon (alpha interferon [IFN-α]). The blockade of DC-lymphocyte cross talk by anti-ICAM-1 antibody markedly inhibited the stimulation of HIV-1 replication and prevented the downregulation of SAMHD1 expression in cocultured DCs. These results demonstrate that, in contrast to purified DCs, cross talk with lymphocytes downregulates SAMHD1 expression in DCs, triggering HIV-1 replication and an antiviral immune response. Therefore, HIV-1 replication and immune sensing by DCs should be investigated in more physiologically relevant models of DC/lymphocyte coculture.
Fil: Su, Bin. Inserm; Francia
Fil: Biedma, Marina Elizabeth. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lederle, Alexandre. Inserm; Francia
Fil: Peressin, Maryse. Inserm; Francia
Fil: Lambotin, Mélanie. Inserm; Francia
Fil: Proust, Alizé. Inserm; Francia
Fil: Decoville, Thomas. Inserm; Francia
Fil: Schmidt, Sylvie. Inserm; Francia
Fil: Laumond, Géraldine. Inserm; Francia
Fil: Moog, Christiane. Inserm; Francia
Materia
Samhd-1
Dcs
Hiv-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31243
Acceder
id CONICETDig_0c2bbd610cfb59986238fcf9afd69116
oai_identifier_str oai:ri.conicet.gov.ar:11336/31243
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic CellsSu, BinBiedma, Marina ElizabethLederle, AlexandrePeressin, MaryseLambotin, MélanieProust, AlizéDecoville, ThomasSchmidt, SylvieLaumond, GéraldineMoog, ChristianeSamhd-1DcsHiv-1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We previously observed that HIV-1 replication in immature DCs is stimulated by cocultivation with primary T and B lymphocytes, suggesting that HIV-1 restriction in DCs may be overcome under coculture conditions. Here, we aimed to decipher the mechanism of SAMHD1-mediated restriction in DC-lymphocyte coculture. We found that coculture with lymphocytes downregulated SAMHD1 expression and was associated with increased HIV-1 replication in DCs. Moreover, in infected DC-T lymphocyte cocultures, DCs acquired maturation status and secreted type 1 interferon (alpha interferon [IFN-α]). The blockade of DC-lymphocyte cross talk by anti-ICAM-1 antibody markedly inhibited the stimulation of HIV-1 replication and prevented the downregulation of SAMHD1 expression in cocultured DCs. These results demonstrate that, in contrast to purified DCs, cross talk with lymphocytes downregulates SAMHD1 expression in DCs, triggering HIV-1 replication and an antiviral immune response. Therefore, HIV-1 replication and immune sensing by DCs should be investigated in more physiologically relevant models of DC/lymphocyte coculture.Fil: Su, Bin. Inserm; FranciaFil: Biedma, Marina Elizabeth. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lederle, Alexandre. Inserm; FranciaFil: Peressin, Maryse. Inserm; FranciaFil: Lambotin, Mélanie. Inserm; FranciaFil: Proust, Alizé. Inserm; FranciaFil: Decoville, Thomas. Inserm; FranciaFil: Schmidt, Sylvie. Inserm; FranciaFil: Laumond, Géraldine. Inserm; FranciaFil: Moog, Christiane. Inserm; FranciaAmerican Society for Microbiology2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31243Moog, Christiane; Laumond, Géraldine; Schmidt, Sylvie; Decoville, Thomas; Proust, Alizé; Lambotin, Mélanie; et al.; Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells; American Society for Microbiology; Journal of Virology; 88; 9; 2-2014; 5109-51210022-538XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/88/9/5109.fullinfo:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.03057-13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:19Zoai:ri.conicet.gov.ar:11336/31243instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:19.739CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
title Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
spellingShingle Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
Su, Bin
Samhd-1
Dcs
Hiv-1
title_short Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
title_full Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
title_fullStr Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
title_full_unstemmed Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
title_sort Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells
dc.creator.none.fl_str_mv Su, Bin
Biedma, Marina Elizabeth
Lederle, Alexandre
Peressin, Maryse
Lambotin, Mélanie
Proust, Alizé
Decoville, Thomas
Schmidt, Sylvie
Laumond, Géraldine
Moog, Christiane
author Su, Bin
author_facet Su, Bin
Biedma, Marina Elizabeth
Lederle, Alexandre
Peressin, Maryse
Lambotin, Mélanie
Proust, Alizé
Decoville, Thomas
Schmidt, Sylvie
Laumond, Géraldine
Moog, Christiane
author_role author
author2 Biedma, Marina Elizabeth
Lederle, Alexandre
Peressin, Maryse
Lambotin, Mélanie
Proust, Alizé
Decoville, Thomas
Schmidt, Sylvie
Laumond, Géraldine
Moog, Christiane
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Samhd-1
Dcs
Hiv-1
topic Samhd-1
Dcs
Hiv-1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We previously observed that HIV-1 replication in immature DCs is stimulated by cocultivation with primary T and B lymphocytes, suggesting that HIV-1 restriction in DCs may be overcome under coculture conditions. Here, we aimed to decipher the mechanism of SAMHD1-mediated restriction in DC-lymphocyte coculture. We found that coculture with lymphocytes downregulated SAMHD1 expression and was associated with increased HIV-1 replication in DCs. Moreover, in infected DC-T lymphocyte cocultures, DCs acquired maturation status and secreted type 1 interferon (alpha interferon [IFN-α]). The blockade of DC-lymphocyte cross talk by anti-ICAM-1 antibody markedly inhibited the stimulation of HIV-1 replication and prevented the downregulation of SAMHD1 expression in cocultured DCs. These results demonstrate that, in contrast to purified DCs, cross talk with lymphocytes downregulates SAMHD1 expression in DCs, triggering HIV-1 replication and an antiviral immune response. Therefore, HIV-1 replication and immune sensing by DCs should be investigated in more physiologically relevant models of DC/lymphocyte coculture.
Fil: Su, Bin. Inserm; Francia
Fil: Biedma, Marina Elizabeth. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lederle, Alexandre. Inserm; Francia
Fil: Peressin, Maryse. Inserm; Francia
Fil: Lambotin, Mélanie. Inserm; Francia
Fil: Proust, Alizé. Inserm; Francia
Fil: Decoville, Thomas. Inserm; Francia
Fil: Schmidt, Sylvie. Inserm; Francia
Fil: Laumond, Géraldine. Inserm; Francia
Fil: Moog, Christiane. Inserm; Francia
description Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We previously observed that HIV-1 replication in immature DCs is stimulated by cocultivation with primary T and B lymphocytes, suggesting that HIV-1 restriction in DCs may be overcome under coculture conditions. Here, we aimed to decipher the mechanism of SAMHD1-mediated restriction in DC-lymphocyte coculture. We found that coculture with lymphocytes downregulated SAMHD1 expression and was associated with increased HIV-1 replication in DCs. Moreover, in infected DC-T lymphocyte cocultures, DCs acquired maturation status and secreted type 1 interferon (alpha interferon [IFN-α]). The blockade of DC-lymphocyte cross talk by anti-ICAM-1 antibody markedly inhibited the stimulation of HIV-1 replication and prevented the downregulation of SAMHD1 expression in cocultured DCs. These results demonstrate that, in contrast to purified DCs, cross talk with lymphocytes downregulates SAMHD1 expression in DCs, triggering HIV-1 replication and an antiviral immune response. Therefore, HIV-1 replication and immune sensing by DCs should be investigated in more physiologically relevant models of DC/lymphocyte coculture.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31243
Moog, Christiane; Laumond, Géraldine; Schmidt, Sylvie; Decoville, Thomas; Proust, Alizé; Lambotin, Mélanie; et al.; Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells; American Society for Microbiology; Journal of Virology; 88; 9; 2-2014; 5109-5121
0022-538X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31243
identifier_str_mv Moog, Christiane; Laumond, Géraldine; Schmidt, Sylvie; Decoville, Thomas; Proust, Alizé; Lambotin, Mélanie; et al.; Dendritic Cell-Lymphocyte Crosstalk Downregulates Host Restriction Factor SAMHD1 and Stimulates HIV-1 Replication in Dendritic Cells; American Society for Microbiology; Journal of Virology; 88; 9; 2-2014; 5109-5121
0022-538X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/88/9/5109.full
info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.03057-13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614405335023616
score 13.069144

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