Are integrins and ligands correlated at pig placental interface during pregnancy?

Autores
Velez, Carolina; Clauzure, Mariangeles; Williamson, Delia Maria; García, Mónica; Koncurat, Mirta Adriana; Barbeito, Claudio Gustavo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface.
Fil: Velez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Clauzure, Mariangeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Williamson, Delia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: García, Mónica. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Koncurat, Mirta Adriana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada;
Materia
FIBRONECTIN
INTEGRINS
OSTEOPONTIN
PIG GESTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224416
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/224416
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Are integrins and ligands correlated at pig placental interface during pregnancy?Velez, CarolinaClauzure, MariangelesWilliamson, Delia MariaGarcía, MónicaKoncurat, Mirta AdrianaBarbeito, Claudio GustavoFIBRONECTININTEGRINSOSTEOPONTINPIG GESTATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface.Fil: Velez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Clauzure, Mariangeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Williamson, Delia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: García, Mónica. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Koncurat, Mirta Adriana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada;BioScientifica Ltd.2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224416Velez, Carolina; Clauzure, Mariangeles; Williamson, Delia Maria; García, Mónica; Koncurat, Mirta Adriana; et al.; Are integrins and ligands correlated at pig placental interface during pregnancy?; BioScientifica Ltd.; Reproduction and Fertility; 4; 1; 2-2023; 1-132633-8386CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://raf.bioscientifica.com/view/journals/raf/aop/raf-22-0079/raf-22-0079.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1530/RAF-22-0079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:03Zoai:ri.conicet.gov.ar:11336/224416instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:04.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Are integrins and ligands correlated at pig placental interface during pregnancy?
title Are integrins and ligands correlated at pig placental interface during pregnancy?
spellingShingle Are integrins and ligands correlated at pig placental interface during pregnancy?
Velez, Carolina
FIBRONECTIN
INTEGRINS
OSTEOPONTIN
PIG GESTATION
title_short Are integrins and ligands correlated at pig placental interface during pregnancy?
title_full Are integrins and ligands correlated at pig placental interface during pregnancy?
title_fullStr Are integrins and ligands correlated at pig placental interface during pregnancy?
title_full_unstemmed Are integrins and ligands correlated at pig placental interface during pregnancy?
title_sort Are integrins and ligands correlated at pig placental interface during pregnancy?
dc.creator.none.fl_str_mv Velez, Carolina
Clauzure, Mariangeles
Williamson, Delia Maria
García, Mónica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
author Velez, Carolina
author_facet Velez, Carolina
Clauzure, Mariangeles
Williamson, Delia Maria
García, Mónica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
author_role author
author2 Clauzure, Mariangeles
Williamson, Delia Maria
García, Mónica
Koncurat, Mirta Adriana
Barbeito, Claudio Gustavo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FIBRONECTIN
INTEGRINS
OSTEOPONTIN
PIG GESTATION
topic FIBRONECTIN
INTEGRINS
OSTEOPONTIN
PIG GESTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface.
Fil: Velez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Clauzure, Mariangeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Williamson, Delia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: García, Mónica. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Koncurat, Mirta Adriana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada;
description In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (n = 24) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (n = 4) of crossbred sows were used. The presence of αvβ3 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and α5β1 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo/feto–maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial αvβ3, and trophoblastic OPN and endometrial α5β1, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine–placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal–fetal interface.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224416
Velez, Carolina; Clauzure, Mariangeles; Williamson, Delia Maria; García, Mónica; Koncurat, Mirta Adriana; et al.; Are integrins and ligands correlated at pig placental interface during pregnancy?; BioScientifica Ltd.; Reproduction and Fertility; 4; 1; 2-2023; 1-13
2633-8386
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224416
identifier_str_mv Velez, Carolina; Clauzure, Mariangeles; Williamson, Delia Maria; García, Mónica; Koncurat, Mirta Adriana; et al.; Are integrins and ligands correlated at pig placental interface during pregnancy?; BioScientifica Ltd.; Reproduction and Fertility; 4; 1; 2-2023; 1-13
2633-8386
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://raf.bioscientifica.com/view/journals/raf/aop/raf-22-0079/raf-22-0079.xml
info:eu-repo/semantics/altIdentifier/doi/10.1530/RAF-22-0079
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica Ltd.
publisher.none.fl_str_mv BioScientifica Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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