Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle

Autores
Leichsenring Silva, Fabiano; Tavares, Angela Maria Vicente; Mosele, Francisca; Berger, Bruno; Llesuy, Susana Francisca; Belló Klein, Adriane
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
1. This study investigates the time course of pulmonary arterial hypertension (PAH) due to monocrotaline (MCT) and its association with cardiac function and oxidative stress markers in the left ventricle (LV). 2. Male Wistar rats were divided into six groups: 7days, 21days, and 31days for both control and MCT groups. Following echocardiographic analysis, the heart was removed. The LV was separated and homogenized to analyze oxidized-to-total glutathione ratio and thioredoxin reductase (TrxR) activity as well as hydrogen peroxide (H 2O 2) and ascorbic acid levels. 3. There was significant (P<0.01) cardiac and right ventricle (RV) hypertrophy and pulmonary congestion in the MCT 21day and 31day groups. Echocardiography showed a change in the flow wave of the pulmonary artery at 21days after MCT treatment. There was an increase in the LV ejection time (P<0.05) at 31days after MCT. The LV H 2O 2 concentration was increased (P<0.05) in the MCT 21day and MCT 31day groups compared with controls. There was a reduction (P<0.05) in the LV ascorbic acid concentration and an increase (P<0.05) in TrxR activity in the MCT 31day rats. 4. Our findings showed RV changes due to pulmonary hypertension at 21days after MCT injection. There was a correlation between the degree of dysfunction and the morphometry of the heart chambers, along with impairment of the antioxidant/pro-oxidant balance in the LV 31days after the beginning of the protocol. This study suggests that LV changes follow RV dysfunction subsequent to pulmonary hypertension.
Fil: Leichsenring Silva, Fabiano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Tavares, Angela Maria Vicente. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mosele, Francisca. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Berger, Bruno. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Materia
Ascorbic Acid
Cor Pulmonale
Glutathione
Monocrotaline
Right Heart Failure
Thioredoxin Reductase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67467
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricleLeichsenring Silva, FabianoTavares, Angela Maria VicenteMosele, FranciscaBerger, BrunoLlesuy, Susana FranciscaBelló Klein, AdrianeAscorbic AcidCor PulmonaleGlutathioneMonocrotalineRight Heart FailureThioredoxin Reductasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/31. This study investigates the time course of pulmonary arterial hypertension (PAH) due to monocrotaline (MCT) and its association with cardiac function and oxidative stress markers in the left ventricle (LV). 2. Male Wistar rats were divided into six groups: 7days, 21days, and 31days for both control and MCT groups. Following echocardiographic analysis, the heart was removed. The LV was separated and homogenized to analyze oxidized-to-total glutathione ratio and thioredoxin reductase (TrxR) activity as well as hydrogen peroxide (H 2O 2) and ascorbic acid levels. 3. There was significant (P<0.01) cardiac and right ventricle (RV) hypertrophy and pulmonary congestion in the MCT 21day and 31day groups. Echocardiography showed a change in the flow wave of the pulmonary artery at 21days after MCT treatment. There was an increase in the LV ejection time (P<0.05) at 31days after MCT. The LV H 2O 2 concentration was increased (P<0.05) in the MCT 21day and MCT 31day groups compared with controls. There was a reduction (P<0.05) in the LV ascorbic acid concentration and an increase (P<0.05) in TrxR activity in the MCT 31day rats. 4. Our findings showed RV changes due to pulmonary hypertension at 21days after MCT injection. There was a correlation between the degree of dysfunction and the morphometry of the heart chambers, along with impairment of the antioxidant/pro-oxidant balance in the LV 31days after the beginning of the protocol. This study suggests that LV changes follow RV dysfunction subsequent to pulmonary hypertension.Fil: Leichsenring Silva, Fabiano. Universidade Federal do Rio Grande do Sul; BrasilFil: Tavares, Angela Maria Vicente. Universidade Federal do Rio Grande do Sul; BrasilFil: Mosele, Francisca. Universidade Federal do Rio Grande do Sul; BrasilFil: Berger, Bruno. Universidade Federal do Rio Grande do Sul; BrasilFil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; BrasilWiley Blackwell Publishing, Inc2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67467Leichsenring Silva, Fabiano; Tavares, Angela Maria Vicente; Mosele, Francisca; Berger, Bruno; Llesuy, Susana Francisca; et al.; Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle; Wiley Blackwell Publishing, Inc; Clinical and Experimental Pharmacology and Physiology; 38; 12; 12-2011; 804-8100305-1870CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1440-1681.2011.05608.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1440-1681.2011.05608.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:56:17Zoai:ri.conicet.gov.ar:11336/67467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:56:17.679CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
title Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
spellingShingle Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
Leichsenring Silva, Fabiano
Ascorbic Acid
Cor Pulmonale
Glutathione
Monocrotaline
Right Heart Failure
Thioredoxin Reductase
title_short Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
title_full Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
title_fullStr Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
title_full_unstemmed Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
title_sort Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle
dc.creator.none.fl_str_mv Leichsenring Silva, Fabiano
Tavares, Angela Maria Vicente
Mosele, Francisca
Berger, Bruno
Llesuy, Susana Francisca
Belló Klein, Adriane
author Leichsenring Silva, Fabiano
author_facet Leichsenring Silva, Fabiano
Tavares, Angela Maria Vicente
Mosele, Francisca
Berger, Bruno
Llesuy, Susana Francisca
Belló Klein, Adriane
author_role author
author2 Tavares, Angela Maria Vicente
Mosele, Francisca
Berger, Bruno
Llesuy, Susana Francisca
Belló Klein, Adriane
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ascorbic Acid
Cor Pulmonale
Glutathione
Monocrotaline
Right Heart Failure
Thioredoxin Reductase
topic Ascorbic Acid
Cor Pulmonale
Glutathione
Monocrotaline
Right Heart Failure
Thioredoxin Reductase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv 1. This study investigates the time course of pulmonary arterial hypertension (PAH) due to monocrotaline (MCT) and its association with cardiac function and oxidative stress markers in the left ventricle (LV). 2. Male Wistar rats were divided into six groups: 7days, 21days, and 31days for both control and MCT groups. Following echocardiographic analysis, the heart was removed. The LV was separated and homogenized to analyze oxidized-to-total glutathione ratio and thioredoxin reductase (TrxR) activity as well as hydrogen peroxide (H 2O 2) and ascorbic acid levels. 3. There was significant (P<0.01) cardiac and right ventricle (RV) hypertrophy and pulmonary congestion in the MCT 21day and 31day groups. Echocardiography showed a change in the flow wave of the pulmonary artery at 21days after MCT treatment. There was an increase in the LV ejection time (P<0.05) at 31days after MCT. The LV H 2O 2 concentration was increased (P<0.05) in the MCT 21day and MCT 31day groups compared with controls. There was a reduction (P<0.05) in the LV ascorbic acid concentration and an increase (P<0.05) in TrxR activity in the MCT 31day rats. 4. Our findings showed RV changes due to pulmonary hypertension at 21days after MCT injection. There was a correlation between the degree of dysfunction and the morphometry of the heart chambers, along with impairment of the antioxidant/pro-oxidant balance in the LV 31days after the beginning of the protocol. This study suggests that LV changes follow RV dysfunction subsequent to pulmonary hypertension.
Fil: Leichsenring Silva, Fabiano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Tavares, Angela Maria Vicente. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mosele, Francisca. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Berger, Bruno. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
description 1. This study investigates the time course of pulmonary arterial hypertension (PAH) due to monocrotaline (MCT) and its association with cardiac function and oxidative stress markers in the left ventricle (LV). 2. Male Wistar rats were divided into six groups: 7days, 21days, and 31days for both control and MCT groups. Following echocardiographic analysis, the heart was removed. The LV was separated and homogenized to analyze oxidized-to-total glutathione ratio and thioredoxin reductase (TrxR) activity as well as hydrogen peroxide (H 2O 2) and ascorbic acid levels. 3. There was significant (P<0.01) cardiac and right ventricle (RV) hypertrophy and pulmonary congestion in the MCT 21day and 31day groups. Echocardiography showed a change in the flow wave of the pulmonary artery at 21days after MCT treatment. There was an increase in the LV ejection time (P<0.05) at 31days after MCT. The LV H 2O 2 concentration was increased (P<0.05) in the MCT 21day and MCT 31day groups compared with controls. There was a reduction (P<0.05) in the LV ascorbic acid concentration and an increase (P<0.05) in TrxR activity in the MCT 31day rats. 4. Our findings showed RV changes due to pulmonary hypertension at 21days after MCT injection. There was a correlation between the degree of dysfunction and the morphometry of the heart chambers, along with impairment of the antioxidant/pro-oxidant balance in the LV 31days after the beginning of the protocol. This study suggests that LV changes follow RV dysfunction subsequent to pulmonary hypertension.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67467
Leichsenring Silva, Fabiano; Tavares, Angela Maria Vicente; Mosele, Francisca; Berger, Bruno; Llesuy, Susana Francisca; et al.; Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle; Wiley Blackwell Publishing, Inc; Clinical and Experimental Pharmacology and Physiology; 38; 12; 12-2011; 804-810
0305-1870
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67467
identifier_str_mv Leichsenring Silva, Fabiano; Tavares, Angela Maria Vicente; Mosele, Francisca; Berger, Bruno; Llesuy, Susana Francisca; et al.; Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle; Wiley Blackwell Publishing, Inc; Clinical and Experimental Pharmacology and Physiology; 38; 12; 12-2011; 804-810
0305-1870
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1440-1681.2011.05608.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1440-1681.2011.05608.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1854322195207028736
score 13.071171

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