Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers

Autores
Gobbato, Nadia Margarita; Rachid, Mirta Maria; Torres Dimani, Rafael Dario; Werenitsky, Cecilia; Lorca, Cecilia; Salva, Maria Susana; Valdéz, Juan Carlos
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Diabetic foot infections and delayed wound healing are suggested as the major therapeutic difficulties, due to the increasing global antimicrobial grugs resistance issues. In previous studies we have shown that topical application of Lactobacillus plantarum ( Lp ) cultures favored the healing process . In this work we investigated the effect of Lp on the microbicidal activity of polymorphonuclear cells (PMNs) infected with Staphylococcus aureus (S. aureus).Material and methods: Diabetic patients with ulcers were treated with a topical application of Lp. Biopsies were taken at day 0, 10 and 30 post- treatments. Histopathological and bacteriological studies were performed. At the same time, circulating blood polymorphonuclear cells (PMNs) were isolated from patients. PMNs were stimulated with Lp and culture supernatant to perform the following tests: phagocytosis by flow cytometry, NETosis by fluorometry and microbicial activity. Methicillin-resistant Staphylococcus aureus (S. aureus) isolated from the ulcer was used as infectious strain in all tests. The effect of Lp on the biofilm and cell viability of S. aureus was measure. Results: PMNs from healthy patients was used as control group. Increased phagocytic activity of PMNs was observed in healthy patients only stimulated with LPS (230 ifm vs 402 ifm p <0.001). The same was also observed for diabetic PMNs (210 ifm vs 460 p <0.001). The PMNs of healthy patients showd greater induction of NETosiss (4000 RFU) vs PMN of diabetics (2000 RFU). A significant increase in NETosis (6000 RFU) and microbicial activity of PMNs of diabetics stimulated with Lp or with supernatant was observed. S. aureus were able to produce biofilm. The effect of Lp on mature biofilm, showed significant differences in terms of biomasa reduction when compared to the controls. Conclusions: The treatment with Lp improve wound healing, suppressingof S. aureus infection at wound sites and promoting host innate immunity.
Fil: Gobbato, Nadia Margarita. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Rachid, Mirta Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torres Dimani, Rafael Dario. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Werenitsky, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Lorca, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Reunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
Materia
LACTOBACILLUS PLANTARUM
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
DIABETES FOOT ULSERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275171
Acceder
id CONICETDig_5b016020134c1ac9cfd84e271fa2b03e
oai_identifier_str oai:ri.conicet.gov.ar:11336/275171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcersGobbato, Nadia MargaritaRachid, Mirta MariaTorres Dimani, Rafael DarioWerenitsky, CeciliaLorca, CeciliaSalva, Maria SusanaValdéz, Juan CarlosLACTOBACILLUS PLANTARUMMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUSDIABETES FOOT ULSERShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Diabetic foot infections and delayed wound healing are suggested as the major therapeutic difficulties, due to the increasing global antimicrobial grugs resistance issues. In previous studies we have shown that topical application of Lactobacillus plantarum ( Lp ) cultures favored the healing process . In this work we investigated the effect of Lp on the microbicidal activity of polymorphonuclear cells (PMNs) infected with Staphylococcus aureus (S. aureus).Material and methods: Diabetic patients with ulcers were treated with a topical application of Lp. Biopsies were taken at day 0, 10 and 30 post- treatments. Histopathological and bacteriological studies were performed. At the same time, circulating blood polymorphonuclear cells (PMNs) were isolated from patients. PMNs were stimulated with Lp and culture supernatant to perform the following tests: phagocytosis by flow cytometry, NETosis by fluorometry and microbicial activity. Methicillin-resistant Staphylococcus aureus (S. aureus) isolated from the ulcer was used as infectious strain in all tests. The effect of Lp on the biofilm and cell viability of S. aureus was measure. Results: PMNs from healthy patients was used as control group. Increased phagocytic activity of PMNs was observed in healthy patients only stimulated with LPS (230 ifm vs 402 ifm p <0.001). The same was also observed for diabetic PMNs (210 ifm vs 460 p <0.001). The PMNs of healthy patients showd greater induction of NETosiss (4000 RFU) vs PMN of diabetics (2000 RFU). A significant increase in NETosis (6000 RFU) and microbicial activity of PMNs of diabetics stimulated with Lp or with supernatant was observed. S. aureus were able to produce biofilm. The effect of Lp on mature biofilm, showed significant differences in terms of biomasa reduction when compared to the controls. Conclusions: The treatment with Lp improve wound healing, suppressingof S. aureus infection at wound sites and promoting host innate immunity.Fil: Gobbato, Nadia Margarita. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Rachid, Mirta Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Torres Dimani, Rafael Dario. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Werenitsky, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Lorca, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaReunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de NanomedicinasCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de Investigación ClínicaAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275171Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers; Reunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 73-730025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:42:02Zoai:ri.conicet.gov.ar:11336/275171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:42:03.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
title Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
spellingShingle Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
Gobbato, Nadia Margarita
LACTOBACILLUS PLANTARUM
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
DIABETES FOOT ULSERS
title_short Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
title_full Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
title_fullStr Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
title_full_unstemmed Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
title_sort Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers
dc.creator.none.fl_str_mv Gobbato, Nadia Margarita
Rachid, Mirta Maria
Torres Dimani, Rafael Dario
Werenitsky, Cecilia
Lorca, Cecilia
Salva, Maria Susana
Valdéz, Juan Carlos
author Gobbato, Nadia Margarita
author_facet Gobbato, Nadia Margarita
Rachid, Mirta Maria
Torres Dimani, Rafael Dario
Werenitsky, Cecilia
Lorca, Cecilia
Salva, Maria Susana
Valdéz, Juan Carlos
author_role author
author2 Rachid, Mirta Maria
Torres Dimani, Rafael Dario
Werenitsky, Cecilia
Lorca, Cecilia
Salva, Maria Susana
Valdéz, Juan Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS PLANTARUM
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
DIABETES FOOT ULSERS
topic LACTOBACILLUS PLANTARUM
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
DIABETES FOOT ULSERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Diabetic foot infections and delayed wound healing are suggested as the major therapeutic difficulties, due to the increasing global antimicrobial grugs resistance issues. In previous studies we have shown that topical application of Lactobacillus plantarum ( Lp ) cultures favored the healing process . In this work we investigated the effect of Lp on the microbicidal activity of polymorphonuclear cells (PMNs) infected with Staphylococcus aureus (S. aureus).Material and methods: Diabetic patients with ulcers were treated with a topical application of Lp. Biopsies were taken at day 0, 10 and 30 post- treatments. Histopathological and bacteriological studies were performed. At the same time, circulating blood polymorphonuclear cells (PMNs) were isolated from patients. PMNs were stimulated with Lp and culture supernatant to perform the following tests: phagocytosis by flow cytometry, NETosis by fluorometry and microbicial activity. Methicillin-resistant Staphylococcus aureus (S. aureus) isolated from the ulcer was used as infectious strain in all tests. The effect of Lp on the biofilm and cell viability of S. aureus was measure. Results: PMNs from healthy patients was used as control group. Increased phagocytic activity of PMNs was observed in healthy patients only stimulated with LPS (230 ifm vs 402 ifm p <0.001). The same was also observed for diabetic PMNs (210 ifm vs 460 p <0.001). The PMNs of healthy patients showd greater induction of NETosiss (4000 RFU) vs PMN of diabetics (2000 RFU). A significant increase in NETosis (6000 RFU) and microbicial activity of PMNs of diabetics stimulated with Lp or with supernatant was observed. S. aureus were able to produce biofilm. The effect of Lp on mature biofilm, showed significant differences in terms of biomasa reduction when compared to the controls. Conclusions: The treatment with Lp improve wound healing, suppressingof S. aureus infection at wound sites and promoting host innate immunity.
Fil: Gobbato, Nadia Margarita. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Rachid, Mirta Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torres Dimani, Rafael Dario. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Werenitsky, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Lorca, Cecilia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Reunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
description Diabetic foot infections and delayed wound healing are suggested as the major therapeutic difficulties, due to the increasing global antimicrobial grugs resistance issues. In previous studies we have shown that topical application of Lactobacillus plantarum ( Lp ) cultures favored the healing process . In this work we investigated the effect of Lp on the microbicidal activity of polymorphonuclear cells (PMNs) infected with Staphylococcus aureus (S. aureus).Material and methods: Diabetic patients with ulcers were treated with a topical application of Lp. Biopsies were taken at day 0, 10 and 30 post- treatments. Histopathological and bacteriological studies were performed. At the same time, circulating blood polymorphonuclear cells (PMNs) were isolated from patients. PMNs were stimulated with Lp and culture supernatant to perform the following tests: phagocytosis by flow cytometry, NETosis by fluorometry and microbicial activity. Methicillin-resistant Staphylococcus aureus (S. aureus) isolated from the ulcer was used as infectious strain in all tests. The effect of Lp on the biofilm and cell viability of S. aureus was measure. Results: PMNs from healthy patients was used as control group. Increased phagocytic activity of PMNs was observed in healthy patients only stimulated with LPS (230 ifm vs 402 ifm p <0.001). The same was also observed for diabetic PMNs (210 ifm vs 460 p <0.001). The PMNs of healthy patients showd greater induction of NETosiss (4000 RFU) vs PMN of diabetics (2000 RFU). A significant increase in NETosis (6000 RFU) and microbicial activity of PMNs of diabetics stimulated with Lp or with supernatant was observed. S. aureus were able to produce biofilm. The effect of Lp on mature biofilm, showed significant differences in terms of biomasa reduction when compared to the controls. Conclusions: The treatment with Lp improve wound healing, suppressingof S. aureus infection at wound sites and promoting host innate immunity.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275171
Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers; Reunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 73-73
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275171
identifier_str_mv Rol of Lactobacillus plantarum in the treatment of methicillin-resistant Staphylococcus aureus infection in diabetic foot ulcers; Reunión de Sociedades de Biociencias 2021: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 73-73
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1850504703050252288
score 13.275514

Publicaciones similares