Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica

Autores
Toranzo, Araceli; Páez, Paulina Laura; Lucero Estrada, Cecilia Stella Marys
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Yersinia enterocolitica is a Gram-negative cocobacillus, not sporulated, mobile at 25 °C but immobile at 37 °C. This pathogenic specie is widely distributed in nature and animals, being the pig the main reservoir of pathogenic strains for humans. Y. enterocolitica can cause numerous diseases, usually at gastrointestinal level but various complications can be manifested especially in immunocompromised people, being the cases where antibacterial therapy is needed. Biofilms are communities of microorganisms that grow irreversibly adhered to living or inert substrates, contained in a polymer matrix secreted by themselves. The most important property of the biofilm forms in clinical medicine is the enhanced resistance to antimicrobial agents. The flagellar motility is crucial initially for surface attachment and subsequently for biofilm formation in Y. enterocolitica. In addition, the fliA gene is a regulator gene necessary for the expression of flagella. The objective of this work was to determine if silver nanoparticles (AgNPs) phytosynthesized from the aqueous extract of Bothriochloa laguroides are capable of inhibiting motility modifying the expression of the fliA gene and eradicating mature biofilm of Y. enterocolitica. Two strains were used: Y. enterocolitica 8081 bio/serotype 1B/O:4 and Y. enterocolitica ME110 1A/O:5. The swimming and swarming motility was determined in a culture medium containing 0.3 and 0.6 % p/v of agar respectively, the fliA gene expression was carried out by RT-PCR and the mature biofilm eradication was determined by the crystal violet technique. The swimming and swarming motility was effectively reduced by AgNPs at 7.8 pM in the two tested strains. The decrease in swimming was 90.38 % for Y. enterocolitica 8081 and 74.27 % for Y. enterocolitica ME110, while for swarming it was 79.16 % and 89.28, respectively. Furthermore, AgNPs at 31.25 pM significantly reduce (p<0.05) the expression of the fliA gene in the two Y. enterocolitica strains. In addition, the AgNPs were able to eradicate mature biofilm at a concentration of 500 pM, with an eradication percentage of 99.33 % for Y. enterocolitica 8081 and 92.95 % for Y. enterocolitica ME110. The AgNPs were able to decrease the motility in Y. enterocolitica and to eradicate the mature biofilm, for which they could be used in the future not only to prevent the formation of biofilm but also to eradicate formed biofilms.
Fil: Toranzo, Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Páez, Paulina Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Buenos Aires
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
SILVER NANOPARTICLES
BIOFILM
YERSINIA ENTEROCOLITA
MOTILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199922
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocoliticaToranzo, AraceliPáez, Paulina LauraLucero Estrada, Cecilia Stella MarysSILVER NANOPARTICLESBIOFILMYERSINIA ENTEROCOLITAMOTILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Yersinia enterocolitica is a Gram-negative cocobacillus, not sporulated, mobile at 25 °C but immobile at 37 °C. This pathogenic specie is widely distributed in nature and animals, being the pig the main reservoir of pathogenic strains for humans. Y. enterocolitica can cause numerous diseases, usually at gastrointestinal level but various complications can be manifested especially in immunocompromised people, being the cases where antibacterial therapy is needed. Biofilms are communities of microorganisms that grow irreversibly adhered to living or inert substrates, contained in a polymer matrix secreted by themselves. The most important property of the biofilm forms in clinical medicine is the enhanced resistance to antimicrobial agents. The flagellar motility is crucial initially for surface attachment and subsequently for biofilm formation in Y. enterocolitica. In addition, the fliA gene is a regulator gene necessary for the expression of flagella. The objective of this work was to determine if silver nanoparticles (AgNPs) phytosynthesized from the aqueous extract of Bothriochloa laguroides are capable of inhibiting motility modifying the expression of the fliA gene and eradicating mature biofilm of Y. enterocolitica. Two strains were used: Y. enterocolitica 8081 bio/serotype 1B/O:4 and Y. enterocolitica ME110 1A/O:5. The swimming and swarming motility was determined in a culture medium containing 0.3 and 0.6 % p/v of agar respectively, the fliA gene expression was carried out by RT-PCR and the mature biofilm eradication was determined by the crystal violet technique. The swimming and swarming motility was effectively reduced by AgNPs at 7.8 pM in the two tested strains. The decrease in swimming was 90.38 % for Y. enterocolitica 8081 and 74.27 % for Y. enterocolitica ME110, while for swarming it was 79.16 % and 89.28, respectively. Furthermore, AgNPs at 31.25 pM significantly reduce (p<0.05) the expression of the fliA gene in the two Y. enterocolitica strains. In addition, the AgNPs were able to eradicate mature biofilm at a concentration of 500 pM, with an eradication percentage of 99.33 % for Y. enterocolitica 8081 and 92.95 % for Y. enterocolitica ME110. The AgNPs were able to decrease the motility in Y. enterocolitica and to eradicate the mature biofilm, for which they could be used in the future not only to prevent the formation of biofilm but also to eradicate formed biofilms.Fil: Toranzo, Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Páez, Paulina Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaLVII SAIB Meeting; XVI SAMIGE MeetingBuenos AiresArgentinaSociedad Argentina De Investigación Bioquímica Y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199922Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 123-1230327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/177-Biocell-Preprint-SAIB-SAMIGE-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:28Zoai:ri.conicet.gov.ar:11336/199922instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:29.327CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
title Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
spellingShingle Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
Toranzo, Araceli
SILVER NANOPARTICLES
BIOFILM
YERSINIA ENTEROCOLITA
MOTILITY
title_short Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
title_full Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
title_fullStr Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
title_full_unstemmed Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
title_sort Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica
dc.creator.none.fl_str_mv Toranzo, Araceli
Páez, Paulina Laura
Lucero Estrada, Cecilia Stella Marys
author Toranzo, Araceli
author_facet Toranzo, Araceli
Páez, Paulina Laura
Lucero Estrada, Cecilia Stella Marys
author_role author
author2 Páez, Paulina Laura
Lucero Estrada, Cecilia Stella Marys
author2_role author
author
dc.subject.none.fl_str_mv SILVER NANOPARTICLES
BIOFILM
YERSINIA ENTEROCOLITA
MOTILITY
topic SILVER NANOPARTICLES
BIOFILM
YERSINIA ENTEROCOLITA
MOTILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Yersinia enterocolitica is a Gram-negative cocobacillus, not sporulated, mobile at 25 °C but immobile at 37 °C. This pathogenic specie is widely distributed in nature and animals, being the pig the main reservoir of pathogenic strains for humans. Y. enterocolitica can cause numerous diseases, usually at gastrointestinal level but various complications can be manifested especially in immunocompromised people, being the cases where antibacterial therapy is needed. Biofilms are communities of microorganisms that grow irreversibly adhered to living or inert substrates, contained in a polymer matrix secreted by themselves. The most important property of the biofilm forms in clinical medicine is the enhanced resistance to antimicrobial agents. The flagellar motility is crucial initially for surface attachment and subsequently for biofilm formation in Y. enterocolitica. In addition, the fliA gene is a regulator gene necessary for the expression of flagella. The objective of this work was to determine if silver nanoparticles (AgNPs) phytosynthesized from the aqueous extract of Bothriochloa laguroides are capable of inhibiting motility modifying the expression of the fliA gene and eradicating mature biofilm of Y. enterocolitica. Two strains were used: Y. enterocolitica 8081 bio/serotype 1B/O:4 and Y. enterocolitica ME110 1A/O:5. The swimming and swarming motility was determined in a culture medium containing 0.3 and 0.6 % p/v of agar respectively, the fliA gene expression was carried out by RT-PCR and the mature biofilm eradication was determined by the crystal violet technique. The swimming and swarming motility was effectively reduced by AgNPs at 7.8 pM in the two tested strains. The decrease in swimming was 90.38 % for Y. enterocolitica 8081 and 74.27 % for Y. enterocolitica ME110, while for swarming it was 79.16 % and 89.28, respectively. Furthermore, AgNPs at 31.25 pM significantly reduce (p<0.05) the expression of the fliA gene in the two Y. enterocolitica strains. In addition, the AgNPs were able to eradicate mature biofilm at a concentration of 500 pM, with an eradication percentage of 99.33 % for Y. enterocolitica 8081 and 92.95 % for Y. enterocolitica ME110. The AgNPs were able to decrease the motility in Y. enterocolitica and to eradicate the mature biofilm, for which they could be used in the future not only to prevent the formation of biofilm but also to eradicate formed biofilms.
Fil: Toranzo, Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Páez, Paulina Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Buenos Aires
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina de Microbiología General
description Yersinia enterocolitica is a Gram-negative cocobacillus, not sporulated, mobile at 25 °C but immobile at 37 °C. This pathogenic specie is widely distributed in nature and animals, being the pig the main reservoir of pathogenic strains for humans. Y. enterocolitica can cause numerous diseases, usually at gastrointestinal level but various complications can be manifested especially in immunocompromised people, being the cases where antibacterial therapy is needed. Biofilms are communities of microorganisms that grow irreversibly adhered to living or inert substrates, contained in a polymer matrix secreted by themselves. The most important property of the biofilm forms in clinical medicine is the enhanced resistance to antimicrobial agents. The flagellar motility is crucial initially for surface attachment and subsequently for biofilm formation in Y. enterocolitica. In addition, the fliA gene is a regulator gene necessary for the expression of flagella. The objective of this work was to determine if silver nanoparticles (AgNPs) phytosynthesized from the aqueous extract of Bothriochloa laguroides are capable of inhibiting motility modifying the expression of the fliA gene and eradicating mature biofilm of Y. enterocolitica. Two strains were used: Y. enterocolitica 8081 bio/serotype 1B/O:4 and Y. enterocolitica ME110 1A/O:5. The swimming and swarming motility was determined in a culture medium containing 0.3 and 0.6 % p/v of agar respectively, the fliA gene expression was carried out by RT-PCR and the mature biofilm eradication was determined by the crystal violet technique. The swimming and swarming motility was effectively reduced by AgNPs at 7.8 pM in the two tested strains. The decrease in swimming was 90.38 % for Y. enterocolitica 8081 and 74.27 % for Y. enterocolitica ME110, while for swarming it was 79.16 % and 89.28, respectively. Furthermore, AgNPs at 31.25 pM significantly reduce (p<0.05) the expression of the fliA gene in the two Y. enterocolitica strains. In addition, the AgNPs were able to eradicate mature biofilm at a concentration of 500 pM, with an eradication percentage of 99.33 % for Y. enterocolitica 8081 and 92.95 % for Y. enterocolitica ME110. The AgNPs were able to decrease the motility in Y. enterocolitica and to eradicate the mature biofilm, for which they could be used in the future not only to prevent the formation of biofilm but also to eradicate formed biofilms.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199922
Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 123-123
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199922
identifier_str_mv Biogenic silver nanoparticles affect motility and eradicate the biofilm in yersinia enterocolitica; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 123-123
0327-9545
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/177-Biocell-Preprint-SAIB-SAMIGE-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
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