Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Autores
Mastrodonato, Anna Chiara; Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Vidal, Roberto; Escudero, María Esther
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42-41,43 (8), B4 O:3 (3) and B1A O:7,8-8-8,19 (2). Autoagglutination (AA), Ca2+-dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA- inv- yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI-PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications: The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.
Fil: Mastrodonato, Anna Chiara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vidal, Roberto. Universidad de Chile; Chile
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Materia
VIRULENCE GENES
ANTIMICROBIAL
GENOMIC DIVERSITY
YERSINIA ENTEROCOLITICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88993
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88993
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and ChileMastrodonato, Anna ChiaraFavier, Gabriela IsabelLucero Estrada, Cecilia Stella MarysVidal, RobertoEscudero, María EstherVIRULENCE GENESANTIMICROBIALGENOMIC DIVERSITYYERSINIA ENTEROCOLITICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42-41,43 (8), B4 O:3 (3) and B1A O:7,8-8-8,19 (2). Autoagglutination (AA), Ca2+-dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA- inv- yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI-PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications: The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.Fil: Mastrodonato, Anna Chiara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Vidal, Roberto. Universidad de Chile; ChileFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaWiley Blackwell Publishing, Inc2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88993Mastrodonato, Anna Chiara; Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Vidal, Roberto; Escudero, María Esther; Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 38; 5; 10-2018; 1-100149-6085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jfs.12491info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jfs.12491info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:04Zoai:ri.conicet.gov.ar:11336/88993instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:05.257CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
title Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
spellingShingle Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
Mastrodonato, Anna Chiara
VIRULENCE GENES
ANTIMICROBIAL
GENOMIC DIVERSITY
YERSINIA ENTEROCOLITICA
title_short Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
title_full Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
title_fullStr Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
title_full_unstemmed Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
title_sort Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile
dc.creator.none.fl_str_mv Mastrodonato, Anna Chiara
Favier, Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Vidal, Roberto
Escudero, María Esther
author Mastrodonato, Anna Chiara
author_facet Mastrodonato, Anna Chiara
Favier, Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Vidal, Roberto
Escudero, María Esther
author_role author
author2 Favier, Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Vidal, Roberto
Escudero, María Esther
author2_role author
author
author
author
dc.subject.none.fl_str_mv VIRULENCE GENES
ANTIMICROBIAL
GENOMIC DIVERSITY
YERSINIA ENTEROCOLITICA
topic VIRULENCE GENES
ANTIMICROBIAL
GENOMIC DIVERSITY
YERSINIA ENTEROCOLITICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42-41,43 (8), B4 O:3 (3) and B1A O:7,8-8-8,19 (2). Autoagglutination (AA), Ca2+-dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA- inv- yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI-PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications: The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.
Fil: Mastrodonato, Anna Chiara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vidal, Roberto. Universidad de Chile; Chile
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
description Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42-41,43 (8), B4 O:3 (3) and B1A O:7,8-8-8,19 (2). Autoagglutination (AA), Ca2+-dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA- inv- yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI-PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications: The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88993
Mastrodonato, Anna Chiara; Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Vidal, Roberto; Escudero, María Esther; Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 38; 5; 10-2018; 1-10
0149-6085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88993
identifier_str_mv Mastrodonato, Anna Chiara; Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Vidal, Roberto; Escudero, María Esther; Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 38; 5; 10-2018; 1-10
0149-6085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jfs.12491
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jfs.12491
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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