Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation

Autores
Ramírez, Valerie T.; Ramos-Fernandez, Eva; Henriquez, Juan Pablo; Lorenzo, Alfredo Guillermo; Inestrosa, Nibaldo C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wnt ligands play crucial roles in the development and regulation of synapse structure and function. Specifically, Wnt-5a acts as a secreted growth factor that regulates dendritic spine formation in rodent hippocampal neurons, resulting in postsynaptic development that promotes the clustering of the PSD-95 (postsynaptic density protein 95). Here, we focused on the early events occurring after the interaction between Wnt-5a and its Frizzled receptor at the neuronal cell surface. Additionally, we studied the role of heterotrimeric G proteins in Wnt-5a-dependent synaptic development. We report that FZD9 (Frizzled9), a Wnt receptor related to Williams syndrome, is localized in the postsynaptic region, where it interacts with Wnt-5a. Functionally, FZD9 is required for the Wnt-5a-mediated increase in dendritic spine density. FZD9 forms a precoupled complex with Gαounder basal conditions that dissociates after Wnt-5a stimulation. Accordingly, we found that G protein inhibition abrogates the Wnt-5a-dependent pathway in hippocampal neurons. In particular, the activation of Gαoappears to be a key factor controlling the Wnt-5a-induced dendritic spine density. In addition, we found that Gβγ is required for the Wnt-5a-mediated increase in cytosolic calcium levels and spinogenesis. Our findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons.
Fil: Ramírez, Valerie T.. Pontificia Universidad Católica de Chile; Chile
Fil: Ramos-Fernandez, Eva. Pontificia Universidad Católica de Chile; Chile
Fil: Henriquez, Juan Pablo. Universidad de Concepción; Chile
Fil: Lorenzo, Alfredo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; Chile
Materia
Wnt signaling
Frizzled Receptor
Dendritic Spine
Heterotrimeric G protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76714
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/76714
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formationRamírez, Valerie T.Ramos-Fernandez, EvaHenriquez, Juan PabloLorenzo, Alfredo GuillermoInestrosa, Nibaldo C.Wnt signalingFrizzled ReceptorDendritic SpineHeterotrimeric G proteinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Wnt ligands play crucial roles in the development and regulation of synapse structure and function. Specifically, Wnt-5a acts as a secreted growth factor that regulates dendritic spine formation in rodent hippocampal neurons, resulting in postsynaptic development that promotes the clustering of the PSD-95 (postsynaptic density protein 95). Here, we focused on the early events occurring after the interaction between Wnt-5a and its Frizzled receptor at the neuronal cell surface. Additionally, we studied the role of heterotrimeric G proteins in Wnt-5a-dependent synaptic development. We report that FZD9 (Frizzled9), a Wnt receptor related to Williams syndrome, is localized in the postsynaptic region, where it interacts with Wnt-5a. Functionally, FZD9 is required for the Wnt-5a-mediated increase in dendritic spine density. FZD9 forms a precoupled complex with Gαounder basal conditions that dissociates after Wnt-5a stimulation. Accordingly, we found that G protein inhibition abrogates the Wnt-5a-dependent pathway in hippocampal neurons. In particular, the activation of Gαoappears to be a key factor controlling the Wnt-5a-induced dendritic spine density. In addition, we found that Gβγ is required for the Wnt-5a-mediated increase in cytosolic calcium levels and spinogenesis. Our findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons.Fil: Ramírez, Valerie T.. Pontificia Universidad Católica de Chile; ChileFil: Ramos-Fernandez, Eva. Pontificia Universidad Católica de Chile; ChileFil: Henriquez, Juan Pablo. Universidad de Concepción; ChileFil: Lorenzo, Alfredo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; ChileAmerican Society for Biochemistry and Molecular Biology2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76714Ramírez, Valerie T.; Ramos-Fernandez, Eva; Henriquez, Juan Pablo; Lorenzo, Alfredo Guillermo; Inestrosa, Nibaldo C.; Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 291; 36; 9-2016; 19092-191070021-92581083-351XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M116.722132info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/27402827info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:00Zoai:ri.conicet.gov.ar:11336/76714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:00.855CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
title Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
spellingShingle Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
Ramírez, Valerie T.
Wnt signaling
Frizzled Receptor
Dendritic Spine
Heterotrimeric G protein
title_short Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
title_full Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
title_fullStr Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
title_full_unstemmed Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
title_sort Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation
dc.creator.none.fl_str_mv Ramírez, Valerie T.
Ramos-Fernandez, Eva
Henriquez, Juan Pablo
Lorenzo, Alfredo Guillermo
Inestrosa, Nibaldo C.
author Ramírez, Valerie T.
author_facet Ramírez, Valerie T.
Ramos-Fernandez, Eva
Henriquez, Juan Pablo
Lorenzo, Alfredo Guillermo
Inestrosa, Nibaldo C.
author_role author
author2 Ramos-Fernandez, Eva
Henriquez, Juan Pablo
Lorenzo, Alfredo Guillermo
Inestrosa, Nibaldo C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Wnt signaling
Frizzled Receptor
Dendritic Spine
Heterotrimeric G protein
topic Wnt signaling
Frizzled Receptor
Dendritic Spine
Heterotrimeric G protein
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Wnt ligands play crucial roles in the development and regulation of synapse structure and function. Specifically, Wnt-5a acts as a secreted growth factor that regulates dendritic spine formation in rodent hippocampal neurons, resulting in postsynaptic development that promotes the clustering of the PSD-95 (postsynaptic density protein 95). Here, we focused on the early events occurring after the interaction between Wnt-5a and its Frizzled receptor at the neuronal cell surface. Additionally, we studied the role of heterotrimeric G proteins in Wnt-5a-dependent synaptic development. We report that FZD9 (Frizzled9), a Wnt receptor related to Williams syndrome, is localized in the postsynaptic region, where it interacts with Wnt-5a. Functionally, FZD9 is required for the Wnt-5a-mediated increase in dendritic spine density. FZD9 forms a precoupled complex with Gαounder basal conditions that dissociates after Wnt-5a stimulation. Accordingly, we found that G protein inhibition abrogates the Wnt-5a-dependent pathway in hippocampal neurons. In particular, the activation of Gαoappears to be a key factor controlling the Wnt-5a-induced dendritic spine density. In addition, we found that Gβγ is required for the Wnt-5a-mediated increase in cytosolic calcium levels and spinogenesis. Our findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons.
Fil: Ramírez, Valerie T.. Pontificia Universidad Católica de Chile; Chile
Fil: Ramos-Fernandez, Eva. Pontificia Universidad Católica de Chile; Chile
Fil: Henriquez, Juan Pablo. Universidad de Concepción; Chile
Fil: Lorenzo, Alfredo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; Chile
description Wnt ligands play crucial roles in the development and regulation of synapse structure and function. Specifically, Wnt-5a acts as a secreted growth factor that regulates dendritic spine formation in rodent hippocampal neurons, resulting in postsynaptic development that promotes the clustering of the PSD-95 (postsynaptic density protein 95). Here, we focused on the early events occurring after the interaction between Wnt-5a and its Frizzled receptor at the neuronal cell surface. Additionally, we studied the role of heterotrimeric G proteins in Wnt-5a-dependent synaptic development. We report that FZD9 (Frizzled9), a Wnt receptor related to Williams syndrome, is localized in the postsynaptic region, where it interacts with Wnt-5a. Functionally, FZD9 is required for the Wnt-5a-mediated increase in dendritic spine density. FZD9 forms a precoupled complex with Gαounder basal conditions that dissociates after Wnt-5a stimulation. Accordingly, we found that G protein inhibition abrogates the Wnt-5a-dependent pathway in hippocampal neurons. In particular, the activation of Gαoappears to be a key factor controlling the Wnt-5a-induced dendritic spine density. In addition, we found that Gβγ is required for the Wnt-5a-mediated increase in cytosolic calcium levels and spinogenesis. Our findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76714
Ramírez, Valerie T.; Ramos-Fernandez, Eva; Henriquez, Juan Pablo; Lorenzo, Alfredo Guillermo; Inestrosa, Nibaldo C.; Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 291; 36; 9-2016; 19092-19107
0021-9258
1083-351X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76714
identifier_str_mv Ramírez, Valerie T.; Ramos-Fernandez, Eva; Henriquez, Juan Pablo; Lorenzo, Alfredo Guillermo; Inestrosa, Nibaldo C.; Wnt-5a/frizzled9 receptor signaling through the Gαo-Gβγ complex regulates dendritic spine formation; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 291; 36; 9-2016; 19092-19107
0021-9258
1083-351X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M116.722132
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/27402827
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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