Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1

Autores
Ciani, Lorenza; Marzo, Aude; Boyle, Kieran; Stamatakou, Eleanna; Lopes, Douglas M.; Anane, Derek; McLeod, Faye; Rosso, Silvana Beatriz; Gibb, Alasdair; Salinas, Patricia C.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The functional assembly of the synaptic release machinery is well understood; however, how signalling factors modulate this process remains unknown. Recent studies suggest that Wnts play a role in presynaptic function. To examine the mechanisms involved, we investigated the interaction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines the cellular locale of Wnt action. Here we show that Dvl1 directly interacts with Synaptotagmin-1 (Syt-1) and indirectly with the SNARE proteins SNAP25 and Syntaxin (Stx-1). Importantly, the interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. Moreover, presynaptic terminals from Wnt signalling-deficient mice exhibit reduced release probability and are unable to sustain high-frequency release. Consistently, the readily releasable pool size and formation of SNARE complexes are reduced. Our studies demonstrate that Wnt signalling tunes neurotransmitter release and identify Syt-1 as a target for modulation by secreted signalling proteins.
Fil: Ciani, Lorenza. University College London; Estados Unidos
Fil: Marzo, Aude. University College London; Estados Unidos
Fil: Boyle, Kieran. University College London; Estados Unidos
Fil: Stamatakou, Eleanna. University College London; Estados Unidos
Fil: Lopes, Douglas M.. University College London; Estados Unidos
Fil: Anane, Derek. University College London; Estados Unidos
Fil: McLeod, Faye. University College London; Estados Unidos
Fil: Rosso, Silvana Beatriz. University College London; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gibb, Alasdair. University College London; Estados Unidos
Fil: Salinas, Patricia C.. University College London; Estados Unidos
Materia
Wnt signaling
Neurotransmitter release
Synapses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15120
Acceder
id CONICETDig_2f70c5220d39061f6444f86c577f8e4b
oai_identifier_str oai:ri.conicet.gov.ar:11336/15120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1Ciani, LorenzaMarzo, AudeBoyle, KieranStamatakou, EleannaLopes, Douglas M.Anane, DerekMcLeod, FayeRosso, Silvana BeatrizGibb, AlasdairSalinas, Patricia C.Wnt signalingNeurotransmitter releaseSynapseshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The functional assembly of the synaptic release machinery is well understood; however, how signalling factors modulate this process remains unknown. Recent studies suggest that Wnts play a role in presynaptic function. To examine the mechanisms involved, we investigated the interaction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines the cellular locale of Wnt action. Here we show that Dvl1 directly interacts with Synaptotagmin-1 (Syt-1) and indirectly with the SNARE proteins SNAP25 and Syntaxin (Stx-1). Importantly, the interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. Moreover, presynaptic terminals from Wnt signalling-deficient mice exhibit reduced release probability and are unable to sustain high-frequency release. Consistently, the readily releasable pool size and formation of SNARE complexes are reduced. Our studies demonstrate that Wnt signalling tunes neurotransmitter release and identify Syt-1 as a target for modulation by secreted signalling proteins.Fil: Ciani, Lorenza. University College London; Estados UnidosFil: Marzo, Aude. University College London; Estados UnidosFil: Boyle, Kieran. University College London; Estados UnidosFil: Stamatakou, Eleanna. University College London; Estados UnidosFil: Lopes, Douglas M.. University College London; Estados UnidosFil: Anane, Derek. University College London; Estados UnidosFil: McLeod, Faye. University College London; Estados UnidosFil: Rosso, Silvana Beatriz. University College London; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gibb, Alasdair. University College London; Estados UnidosFil: Salinas, Patricia C.. University College London; Estados UnidosMacmillan Publishers2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15120Ciani, Lorenza; Marzo, Aude; Boyle, Kieran; Stamatakou, Eleanna; Lopes, Douglas M.; et al.; Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1; Macmillan Publishers; Nature Communications; 6; -1-2015; 1-13; 83022041-1723enginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9302info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/ncomms9302info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667432/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:19Zoai:ri.conicet.gov.ar:11336/15120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:19.608CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
title Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
spellingShingle Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
Ciani, Lorenza
Wnt signaling
Neurotransmitter release
Synapses
title_short Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
title_full Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
title_fullStr Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
title_full_unstemmed Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
title_sort Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1
dc.creator.none.fl_str_mv Ciani, Lorenza
Marzo, Aude
Boyle, Kieran
Stamatakou, Eleanna
Lopes, Douglas M.
Anane, Derek
McLeod, Faye
Rosso, Silvana Beatriz
Gibb, Alasdair
Salinas, Patricia C.
author Ciani, Lorenza
author_facet Ciani, Lorenza
Marzo, Aude
Boyle, Kieran
Stamatakou, Eleanna
Lopes, Douglas M.
Anane, Derek
McLeod, Faye
Rosso, Silvana Beatriz
Gibb, Alasdair
Salinas, Patricia C.
author_role author
author2 Marzo, Aude
Boyle, Kieran
Stamatakou, Eleanna
Lopes, Douglas M.
Anane, Derek
McLeod, Faye
Rosso, Silvana Beatriz
Gibb, Alasdair
Salinas, Patricia C.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wnt signaling
Neurotransmitter release
Synapses
topic Wnt signaling
Neurotransmitter release
Synapses
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The functional assembly of the synaptic release machinery is well understood; however, how signalling factors modulate this process remains unknown. Recent studies suggest that Wnts play a role in presynaptic function. To examine the mechanisms involved, we investigated the interaction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines the cellular locale of Wnt action. Here we show that Dvl1 directly interacts with Synaptotagmin-1 (Syt-1) and indirectly with the SNARE proteins SNAP25 and Syntaxin (Stx-1). Importantly, the interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. Moreover, presynaptic terminals from Wnt signalling-deficient mice exhibit reduced release probability and are unable to sustain high-frequency release. Consistently, the readily releasable pool size and formation of SNARE complexes are reduced. Our studies demonstrate that Wnt signalling tunes neurotransmitter release and identify Syt-1 as a target for modulation by secreted signalling proteins.
Fil: Ciani, Lorenza. University College London; Estados Unidos
Fil: Marzo, Aude. University College London; Estados Unidos
Fil: Boyle, Kieran. University College London; Estados Unidos
Fil: Stamatakou, Eleanna. University College London; Estados Unidos
Fil: Lopes, Douglas M.. University College London; Estados Unidos
Fil: Anane, Derek. University College London; Estados Unidos
Fil: McLeod, Faye. University College London; Estados Unidos
Fil: Rosso, Silvana Beatriz. University College London; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gibb, Alasdair. University College London; Estados Unidos
Fil: Salinas, Patricia C.. University College London; Estados Unidos
description The functional assembly of the synaptic release machinery is well understood; however, how signalling factors modulate this process remains unknown. Recent studies suggest that Wnts play a role in presynaptic function. To examine the mechanisms involved, we investigated the interaction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines the cellular locale of Wnt action. Here we show that Dvl1 directly interacts with Synaptotagmin-1 (Syt-1) and indirectly with the SNARE proteins SNAP25 and Syntaxin (Stx-1). Importantly, the interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. Moreover, presynaptic terminals from Wnt signalling-deficient mice exhibit reduced release probability and are unable to sustain high-frequency release. Consistently, the readily releasable pool size and formation of SNARE complexes are reduced. Our studies demonstrate that Wnt signalling tunes neurotransmitter release and identify Syt-1 as a target for modulation by secreted signalling proteins.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15120
Ciani, Lorenza; Marzo, Aude; Boyle, Kieran; Stamatakou, Eleanna; Lopes, Douglas M.; et al.; Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1; Macmillan Publishers; Nature Communications; 6; -1-2015; 1-13; 8302
2041-1723
url http://hdl.handle.net/11336/15120
identifier_str_mv Ciani, Lorenza; Marzo, Aude; Boyle, Kieran; Stamatakou, Eleanna; Lopes, Douglas M.; et al.; Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1; Macmillan Publishers; Nature Communications; 6; -1-2015; 1-13; 8302
2041-1723
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9302
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/ncomms9302
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667432/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Macmillan Publishers
publisher.none.fl_str_mv Macmillan Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269998644461568
score 13.13397

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