Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor

Autores
Bonecchi, Raffaella; Locati, Massimo; Galliera, Emanuela; Vulcano, Marisa; Sironi, Marina; Fra, Anna M; Gobbi, Marco; Vecchi, Annunciata; Sozzani, Silvano; Haribabu, Bodduluri; Van Damme, Jo; Mantovani, Alberto
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The promiscuous D6 receptor binds several inflammatory CC chemokines and has been recently proposed to act as a chemokinescavenging decoy receptor. The present study was designed to better characterize the spectrum of CC chemokines scavenged by D6, focusing in particular on CCR4 ligands and analyzing the influence of NH2-terminal processing on recognition by this promiscuous receptor. Using D6 transfectants, it was found that D6 efficiently bound and scavenged most inflammatory CC chemokines (CCR1 through CCR5 agonists). Homeostatic CC chemokines (CCR6 and CCR7 agonists) were not recognized by D6. The CCR4 agonists CC chemokine ligand 17 (CCL17) and CCL22 bound to D6 with high affinity. CCL17 and CCL22 have no agonistic activity for D6 (chemotaxis and calcium fluxes), but were rapidly scavenged, resulting in reduced chemotactic activity on CCR4 transfectants. CD26 mediates NH2 terminus processing of CCL22, leading to the production of CCL22 (3–69) and CCL22 (5–69) that do not interact with CCR4. These NH2-terminal truncated forms of CCL22 were not recognized by D6. The results presented in this study show that D6 recognizes and scavenges a wide spectrum of inflammatory CC chemokines, including the CCR4 agonists CCL22 and CCL17. However, this promiscuous receptor is not engaged by CD26-processed, inactive, CCL22 variants. By recognizing intact CCL22, but not its truncated variants, D6 expressed on lymphatic endothelial cells may regulate the traffic of CCR4-expressing cells, such as dendritic cells. T
Fil: Bonecchi, Raffaella. University of Brescia; Italia
Fil: Locati, Massimo. Università degli Studi di Milano; Italia
Fil: Galliera, Emanuela. Università degli Studi di Milano; Italia
Fil: Vulcano, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Mario Negri Institute; Italia
Fil: Sironi, Marina. Mario Negri Institute; Italia
Fil: Fra, Anna M. University of Brescia; Italia
Fil: Gobbi, Marco. Mario Negri Institute; Italia
Fil: Vecchi, Annunciata. Mario Negri Institute; Italia
Fil: Sozzani, Silvano. University of Brescia; Italia. Mario Negri Institute; Italia
Fil: Haribabu, Bodduluri. University of Louisville; Estados Unidos
Fil: Van Damme, Jo. Rega Institute for Medical Research; Bélgica
Fil: Mantovani, Alberto. Università degli Studi di Milano; Italia. Mario Negri Institute; Italia
Materia
D6 RECEPTOR
CC CHEMOKINES
SCAVENGING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257665
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/257665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy ReceptorBonecchi, RaffaellaLocati, MassimoGalliera, EmanuelaVulcano, MarisaSironi, MarinaFra, Anna MGobbi, MarcoVecchi, AnnunciataSozzani, SilvanoHaribabu, BodduluriVan Damme, JoMantovani, AlbertoD6 RECEPTORCC CHEMOKINESSCAVENGINGhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The promiscuous D6 receptor binds several inflammatory CC chemokines and has been recently proposed to act as a chemokinescavenging decoy receptor. The present study was designed to better characterize the spectrum of CC chemokines scavenged by D6, focusing in particular on CCR4 ligands and analyzing the influence of NH2-terminal processing on recognition by this promiscuous receptor. Using D6 transfectants, it was found that D6 efficiently bound and scavenged most inflammatory CC chemokines (CCR1 through CCR5 agonists). Homeostatic CC chemokines (CCR6 and CCR7 agonists) were not recognized by D6. The CCR4 agonists CC chemokine ligand 17 (CCL17) and CCL22 bound to D6 with high affinity. CCL17 and CCL22 have no agonistic activity for D6 (chemotaxis and calcium fluxes), but were rapidly scavenged, resulting in reduced chemotactic activity on CCR4 transfectants. CD26 mediates NH2 terminus processing of CCL22, leading to the production of CCL22 (3–69) and CCL22 (5–69) that do not interact with CCR4. These NH2-terminal truncated forms of CCL22 were not recognized by D6. The results presented in this study show that D6 recognizes and scavenges a wide spectrum of inflammatory CC chemokines, including the CCR4 agonists CCL22 and CCL17. However, this promiscuous receptor is not engaged by CD26-processed, inactive, CCL22 variants. By recognizing intact CCL22, but not its truncated variants, D6 expressed on lymphatic endothelial cells may regulate the traffic of CCR4-expressing cells, such as dendritic cells. TFil: Bonecchi, Raffaella. University of Brescia; ItaliaFil: Locati, Massimo. Università degli Studi di Milano; ItaliaFil: Galliera, Emanuela. Università degli Studi di Milano; ItaliaFil: Vulcano, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Mario Negri Institute; ItaliaFil: Sironi, Marina. Mario Negri Institute; ItaliaFil: Fra, Anna M. University of Brescia; ItaliaFil: Gobbi, Marco. Mario Negri Institute; ItaliaFil: Vecchi, Annunciata. Mario Negri Institute; ItaliaFil: Sozzani, Silvano. University of Brescia; Italia. Mario Negri Institute; ItaliaFil: Haribabu, Bodduluri. University of Louisville; Estados UnidosFil: Van Damme, Jo. Rega Institute for Medical Research; BélgicaFil: Mantovani, Alberto. Università degli Studi di Milano; Italia. Mario Negri Institute; ItaliaAmerican Association of Immunologists2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257665Bonecchi, Raffaella; Locati, Massimo; Galliera, Emanuela; Vulcano, Marisa; Sironi, Marina; et al.; Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor; American Association of Immunologists; Journal of Immunology; 172; 8; 12-2004; 4972-49760022-1767CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.aai.org/jimmunol/article/172/8/4972/35828/Differential-Recognition-and-Scavenging-of-Nativeinfo:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.172.8.4972info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:21Zoai:ri.conicet.gov.ar:11336/257665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:21.327CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
title Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
spellingShingle Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
Bonecchi, Raffaella
D6 RECEPTOR
CC CHEMOKINES
SCAVENGING
title_short Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
title_full Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
title_fullStr Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
title_full_unstemmed Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
title_sort Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor
dc.creator.none.fl_str_mv Bonecchi, Raffaella
Locati, Massimo
Galliera, Emanuela
Vulcano, Marisa
Sironi, Marina
Fra, Anna M
Gobbi, Marco
Vecchi, Annunciata
Sozzani, Silvano
Haribabu, Bodduluri
Van Damme, Jo
Mantovani, Alberto
author Bonecchi, Raffaella
author_facet Bonecchi, Raffaella
Locati, Massimo
Galliera, Emanuela
Vulcano, Marisa
Sironi, Marina
Fra, Anna M
Gobbi, Marco
Vecchi, Annunciata
Sozzani, Silvano
Haribabu, Bodduluri
Van Damme, Jo
Mantovani, Alberto
author_role author
author2 Locati, Massimo
Galliera, Emanuela
Vulcano, Marisa
Sironi, Marina
Fra, Anna M
Gobbi, Marco
Vecchi, Annunciata
Sozzani, Silvano
Haribabu, Bodduluri
Van Damme, Jo
Mantovani, Alberto
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv D6 RECEPTOR
CC CHEMOKINES
SCAVENGING
topic D6 RECEPTOR
CC CHEMOKINES
SCAVENGING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The promiscuous D6 receptor binds several inflammatory CC chemokines and has been recently proposed to act as a chemokinescavenging decoy receptor. The present study was designed to better characterize the spectrum of CC chemokines scavenged by D6, focusing in particular on CCR4 ligands and analyzing the influence of NH2-terminal processing on recognition by this promiscuous receptor. Using D6 transfectants, it was found that D6 efficiently bound and scavenged most inflammatory CC chemokines (CCR1 through CCR5 agonists). Homeostatic CC chemokines (CCR6 and CCR7 agonists) were not recognized by D6. The CCR4 agonists CC chemokine ligand 17 (CCL17) and CCL22 bound to D6 with high affinity. CCL17 and CCL22 have no agonistic activity for D6 (chemotaxis and calcium fluxes), but were rapidly scavenged, resulting in reduced chemotactic activity on CCR4 transfectants. CD26 mediates NH2 terminus processing of CCL22, leading to the production of CCL22 (3–69) and CCL22 (5–69) that do not interact with CCR4. These NH2-terminal truncated forms of CCL22 were not recognized by D6. The results presented in this study show that D6 recognizes and scavenges a wide spectrum of inflammatory CC chemokines, including the CCR4 agonists CCL22 and CCL17. However, this promiscuous receptor is not engaged by CD26-processed, inactive, CCL22 variants. By recognizing intact CCL22, but not its truncated variants, D6 expressed on lymphatic endothelial cells may regulate the traffic of CCR4-expressing cells, such as dendritic cells. T
Fil: Bonecchi, Raffaella. University of Brescia; Italia
Fil: Locati, Massimo. Università degli Studi di Milano; Italia
Fil: Galliera, Emanuela. Università degli Studi di Milano; Italia
Fil: Vulcano, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Mario Negri Institute; Italia
Fil: Sironi, Marina. Mario Negri Institute; Italia
Fil: Fra, Anna M. University of Brescia; Italia
Fil: Gobbi, Marco. Mario Negri Institute; Italia
Fil: Vecchi, Annunciata. Mario Negri Institute; Italia
Fil: Sozzani, Silvano. University of Brescia; Italia. Mario Negri Institute; Italia
Fil: Haribabu, Bodduluri. University of Louisville; Estados Unidos
Fil: Van Damme, Jo. Rega Institute for Medical Research; Bélgica
Fil: Mantovani, Alberto. Università degli Studi di Milano; Italia. Mario Negri Institute; Italia
description The promiscuous D6 receptor binds several inflammatory CC chemokines and has been recently proposed to act as a chemokinescavenging decoy receptor. The present study was designed to better characterize the spectrum of CC chemokines scavenged by D6, focusing in particular on CCR4 ligands and analyzing the influence of NH2-terminal processing on recognition by this promiscuous receptor. Using D6 transfectants, it was found that D6 efficiently bound and scavenged most inflammatory CC chemokines (CCR1 through CCR5 agonists). Homeostatic CC chemokines (CCR6 and CCR7 agonists) were not recognized by D6. The CCR4 agonists CC chemokine ligand 17 (CCL17) and CCL22 bound to D6 with high affinity. CCL17 and CCL22 have no agonistic activity for D6 (chemotaxis and calcium fluxes), but were rapidly scavenged, resulting in reduced chemotactic activity on CCR4 transfectants. CD26 mediates NH2 terminus processing of CCL22, leading to the production of CCL22 (3–69) and CCL22 (5–69) that do not interact with CCR4. These NH2-terminal truncated forms of CCL22 were not recognized by D6. The results presented in this study show that D6 recognizes and scavenges a wide spectrum of inflammatory CC chemokines, including the CCR4 agonists CCL22 and CCL17. However, this promiscuous receptor is not engaged by CD26-processed, inactive, CCL22 variants. By recognizing intact CCL22, but not its truncated variants, D6 expressed on lymphatic endothelial cells may regulate the traffic of CCR4-expressing cells, such as dendritic cells. T
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257665
Bonecchi, Raffaella; Locati, Massimo; Galliera, Emanuela; Vulcano, Marisa; Sironi, Marina; et al.; Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor; American Association of Immunologists; Journal of Immunology; 172; 8; 12-2004; 4972-4976
0022-1767
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257665
identifier_str_mv Bonecchi, Raffaella; Locati, Massimo; Galliera, Emanuela; Vulcano, Marisa; Sironi, Marina; et al.; Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor; American Association of Immunologists; Journal of Immunology; 172; 8; 12-2004; 4972-4976
0022-1767
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.aai.org/jimmunol/article/172/8/4972/35828/Differential-Recognition-and-Scavenging-of-Native
info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.172.8.4972
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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