Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode

Autores
Paz Zanini, Veronica Irene; Gimenez, Rodrigo Esteban; Linarez Pérez, Omar Ezequiel; Lopez, Beatriz Alicia; Borsarelli, Claudio Darío
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simply prepared, low-cost, and sensitive electrochemically activated glassy carbon electrode (GCEa) modified with absorbed chitosan (CHIT) film for quantification of tryptophan (Trp) is reported. Combination of cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for characterization of the electro-oxidation of the amino acid and the electro-analytical performance of the CHIT-modified electrode. The electro-oxidation of Trp involves an irreversible two-electron and two-proton transfer process in both bare and modified electrodes, but the adsorption of CHIT as a polycation onto GCEa produces a ≈4-fold increase of the oxidation current of Trp without changing both the oxidation potential and the heterogeneous reaction rate constant, suggesting that the biopolymer behaves as a proton relay species, probably due to hydrogen bonding/proton acceptor capability of hydroxyl and ether groups of CHIT. Finally, the electro-analytical features of the CHIT-modified electrode as Trp sensor were also evaluated, obtaining a linear response range up to 130 μM Trp, sensitivity of 0.68 μA μM−1 and detection limit of 0.04 μM Trp, with almost no interference of other amino acids.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Gimenez, Rodrigo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Linarez Pérez, Omar Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Materia
Tryptophan
Chitosan
Modified Glassy Carbon Electrodes
Electro-Oxidation
Proton Relay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31500
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31500
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrodePaz Zanini, Veronica IreneGimenez, Rodrigo EstebanLinarez Pérez, Omar EzequielLopez, Beatriz AliciaBorsarelli, Claudio DaríoTryptophanChitosanModified Glassy Carbon ElectrodesElectro-OxidationProton Relayhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A simply prepared, low-cost, and sensitive electrochemically activated glassy carbon electrode (GCEa) modified with absorbed chitosan (CHIT) film for quantification of tryptophan (Trp) is reported. Combination of cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for characterization of the electro-oxidation of the amino acid and the electro-analytical performance of the CHIT-modified electrode. The electro-oxidation of Trp involves an irreversible two-electron and two-proton transfer process in both bare and modified electrodes, but the adsorption of CHIT as a polycation onto GCEa produces a ≈4-fold increase of the oxidation current of Trp without changing both the oxidation potential and the heterogeneous reaction rate constant, suggesting that the biopolymer behaves as a proton relay species, probably due to hydrogen bonding/proton acceptor capability of hydroxyl and ether groups of CHIT. Finally, the electro-analytical features of the CHIT-modified electrode as Trp sensor were also evaluated, obtaining a linear response range up to 130 μM Trp, sensitivity of 0.68 μA μM−1 and detection limit of 0.04 μM Trp, with almost no interference of other amino acids.Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Gimenez, Rodrigo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Linarez Pérez, Omar Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaElsevier Science Sa2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31500Borsarelli, Claudio Darío; Lopez, Beatriz Alicia; Linarez Pérez, Omar Ezequiel; Gimenez, Rodrigo Esteban; Paz Zanini, Veronica Irene; Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode; Elsevier Science Sa; Sensors and Actuators B: Chemical; 209; 11-2014; 391-3980925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.11.087info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400514014701info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:53Zoai:ri.conicet.gov.ar:11336/31500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:53.405CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
title Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
spellingShingle Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
Paz Zanini, Veronica Irene
Tryptophan
Chitosan
Modified Glassy Carbon Electrodes
Electro-Oxidation
Proton Relay
title_short Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
title_full Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
title_fullStr Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
title_full_unstemmed Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
title_sort Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode
dc.creator.none.fl_str_mv Paz Zanini, Veronica Irene
Gimenez, Rodrigo Esteban
Linarez Pérez, Omar Ezequiel
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author Paz Zanini, Veronica Irene
author_facet Paz Zanini, Veronica Irene
Gimenez, Rodrigo Esteban
Linarez Pérez, Omar Ezequiel
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author_role author
author2 Gimenez, Rodrigo Esteban
Linarez Pérez, Omar Ezequiel
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author2_role author
author
author
author
dc.subject.none.fl_str_mv Tryptophan
Chitosan
Modified Glassy Carbon Electrodes
Electro-Oxidation
Proton Relay
topic Tryptophan
Chitosan
Modified Glassy Carbon Electrodes
Electro-Oxidation
Proton Relay
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A simply prepared, low-cost, and sensitive electrochemically activated glassy carbon electrode (GCEa) modified with absorbed chitosan (CHIT) film for quantification of tryptophan (Trp) is reported. Combination of cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for characterization of the electro-oxidation of the amino acid and the electro-analytical performance of the CHIT-modified electrode. The electro-oxidation of Trp involves an irreversible two-electron and two-proton transfer process in both bare and modified electrodes, but the adsorption of CHIT as a polycation onto GCEa produces a ≈4-fold increase of the oxidation current of Trp without changing both the oxidation potential and the heterogeneous reaction rate constant, suggesting that the biopolymer behaves as a proton relay species, probably due to hydrogen bonding/proton acceptor capability of hydroxyl and ether groups of CHIT. Finally, the electro-analytical features of the CHIT-modified electrode as Trp sensor were also evaluated, obtaining a linear response range up to 130 μM Trp, sensitivity of 0.68 μA μM−1 and detection limit of 0.04 μM Trp, with almost no interference of other amino acids.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Gimenez, Rodrigo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Linarez Pérez, Omar Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
description A simply prepared, low-cost, and sensitive electrochemically activated glassy carbon electrode (GCEa) modified with absorbed chitosan (CHIT) film for quantification of tryptophan (Trp) is reported. Combination of cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for characterization of the electro-oxidation of the amino acid and the electro-analytical performance of the CHIT-modified electrode. The electro-oxidation of Trp involves an irreversible two-electron and two-proton transfer process in both bare and modified electrodes, but the adsorption of CHIT as a polycation onto GCEa produces a ≈4-fold increase of the oxidation current of Trp without changing both the oxidation potential and the heterogeneous reaction rate constant, suggesting that the biopolymer behaves as a proton relay species, probably due to hydrogen bonding/proton acceptor capability of hydroxyl and ether groups of CHIT. Finally, the electro-analytical features of the CHIT-modified electrode as Trp sensor were also evaluated, obtaining a linear response range up to 130 μM Trp, sensitivity of 0.68 μA μM−1 and detection limit of 0.04 μM Trp, with almost no interference of other amino acids.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31500
Borsarelli, Claudio Darío; Lopez, Beatriz Alicia; Linarez Pérez, Omar Ezequiel; Gimenez, Rodrigo Esteban; Paz Zanini, Veronica Irene; Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode; Elsevier Science Sa; Sensors and Actuators B: Chemical; 209; 11-2014; 391-398
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31500
identifier_str_mv Borsarelli, Claudio Darío; Lopez, Beatriz Alicia; Linarez Pérez, Omar Ezequiel; Gimenez, Rodrigo Esteban; Paz Zanini, Veronica Irene; Enhancement of amperometric response to tryptophan by proton relay effect of chitosan adsorbed on glassy carbon electrode; Elsevier Science Sa; Sensors and Actuators B: Chemical; 209; 11-2014; 391-398
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.11.087
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400514014701
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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