Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction

Autores
Arias, Paulina; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Bollo, Soledad
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion, modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules.
Fil: Arias, Paulina. Universidad de Chile; Chile
Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bollo, Soledad. Universidad de Chile; Chile
Materia
Carbon Nanotubes
Chitosan
Dna Damage
Glassy Carbon Electrode
Methylene Blue Electrochemistry
Methylene Blue Sensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82855
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interactionArias, PaulinaFerreyra, Nancy FabianaRivas, Gustavo AdolfoBollo, SoledadCarbon NanotubesChitosanDna DamageGlassy Carbon ElectrodeMethylene Blue ElectrochemistryMethylene Blue Sensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion, modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules.Fil: Arias, Paulina. Universidad de Chile; ChileFil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bollo, Soledad. Universidad de Chile; ChileElsevier Science Sa2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82855Arias, Paulina; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Bollo, Soledad; Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 634; 2; 9-2009; 123-1261572-6657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022072809002800info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2009.07.022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:55:23Zoai:ri.conicet.gov.ar:11336/82855instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:55:23.789CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
title Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
spellingShingle Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
Arias, Paulina
Carbon Nanotubes
Chitosan
Dna Damage
Glassy Carbon Electrode
Methylene Blue Electrochemistry
Methylene Blue Sensor
title_short Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
title_full Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
title_fullStr Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
title_full_unstemmed Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
title_sort Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
dc.creator.none.fl_str_mv Arias, Paulina
Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
Bollo, Soledad
author Arias, Paulina
author_facet Arias, Paulina
Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
Bollo, Soledad
author_role author
author2 Ferreyra, Nancy Fabiana
Rivas, Gustavo Adolfo
Bollo, Soledad
author2_role author
author
author
dc.subject.none.fl_str_mv Carbon Nanotubes
Chitosan
Dna Damage
Glassy Carbon Electrode
Methylene Blue Electrochemistry
Methylene Blue Sensor
topic Carbon Nanotubes
Chitosan
Dna Damage
Glassy Carbon Electrode
Methylene Blue Electrochemistry
Methylene Blue Sensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion, modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules.
Fil: Arias, Paulina. Universidad de Chile; Chile
Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bollo, Soledad. Universidad de Chile; Chile
description In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion, modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82855
Arias, Paulina; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Bollo, Soledad; Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 634; 2; 9-2009; 123-126
1572-6657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82855
identifier_str_mv Arias, Paulina; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Bollo, Soledad; Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 634; 2; 9-2009; 123-126
1572-6657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022072809002800
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2009.07.022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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