Adsorption and electrooxidation of DNA at glassy carbon paste electrodes

Autores
Pedano, Maria Laura; Rivas, Gustavo Adolfo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the study of the adsorption and electrooxidation of nucleic acids on glassy carbon paste electrodes (GCPE) by using chronopotentiometric stripping analysis. The influence of electrochemical pretreatments, binder content, supporting electrolyte, accumulation potential, and time on the adsorption and further electrooxidation of oligo(dG)21 is discussed. The adsorption behavior of double stranded calf thymus DNA (dsDNA) on GCPE is also evaluated. Trace (ppb) levels of oligo(dG)21 and (ppm) levels of dsDNA can be readily detected following short accumulation periods, with detection limits of 21 ppb and 200 ppb for oligo(dG)21 and dsDNA, respectively. The confined DNA layer demonstrated to be stable in air, in 0.200M acetate buffer pH 5.00, and in 0.020M phosphate buffer pH 7.40+0.50M NaCl.
Fil: Pedano, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Dna Adsorption
Dna Electrooxidation
Dna-Biosensors
Dna-Modified Electrode
Glassy Carbon Paste Electrode
Potentiometric Stripping Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71302
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Adsorption and electrooxidation of DNA at glassy carbon paste electrodesPedano, Maria LauraRivas, Gustavo AdolfoDna AdsorptionDna ElectrooxidationDna-BiosensorsDna-Modified ElectrodeGlassy Carbon Paste ElectrodePotentiometric Stripping Analysishttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the study of the adsorption and electrooxidation of nucleic acids on glassy carbon paste electrodes (GCPE) by using chronopotentiometric stripping analysis. The influence of electrochemical pretreatments, binder content, supporting electrolyte, accumulation potential, and time on the adsorption and further electrooxidation of oligo(dG)21 is discussed. The adsorption behavior of double stranded calf thymus DNA (dsDNA) on GCPE is also evaluated. Trace (ppb) levels of oligo(dG)21 and (ppm) levels of dsDNA can be readily detected following short accumulation periods, with detection limits of 21 ppb and 200 ppb for oligo(dG)21 and dsDNA, respectively. The confined DNA layer demonstrated to be stable in air, in 0.200M acetate buffer pH 5.00, and in 0.020M phosphate buffer pH 7.40+0.50M NaCl.Fil: Pedano, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaTaylor2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71302Pedano, Maria Laura; Rivas, Gustavo Adolfo; Adsorption and electrooxidation of DNA at glassy carbon paste electrodes; Taylor ; Analytical Letters; 43; 10-11; 7-2010; 1703-17120003-2719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/00032711003653866info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032711003653866info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:32Zoai:ri.conicet.gov.ar:11336/71302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:32.447CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
title Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
spellingShingle Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
Pedano, Maria Laura
Dna Adsorption
Dna Electrooxidation
Dna-Biosensors
Dna-Modified Electrode
Glassy Carbon Paste Electrode
Potentiometric Stripping Analysis
title_short Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
title_full Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
title_fullStr Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
title_full_unstemmed Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
title_sort Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
dc.creator.none.fl_str_mv Pedano, Maria Laura
Rivas, Gustavo Adolfo
author Pedano, Maria Laura
author_facet Pedano, Maria Laura
Rivas, Gustavo Adolfo
author_role author
author2 Rivas, Gustavo Adolfo
author2_role author
dc.subject.none.fl_str_mv Dna Adsorption
Dna Electrooxidation
Dna-Biosensors
Dna-Modified Electrode
Glassy Carbon Paste Electrode
Potentiometric Stripping Analysis
topic Dna Adsorption
Dna Electrooxidation
Dna-Biosensors
Dna-Modified Electrode
Glassy Carbon Paste Electrode
Potentiometric Stripping Analysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the study of the adsorption and electrooxidation of nucleic acids on glassy carbon paste electrodes (GCPE) by using chronopotentiometric stripping analysis. The influence of electrochemical pretreatments, binder content, supporting electrolyte, accumulation potential, and time on the adsorption and further electrooxidation of oligo(dG)21 is discussed. The adsorption behavior of double stranded calf thymus DNA (dsDNA) on GCPE is also evaluated. Trace (ppb) levels of oligo(dG)21 and (ppm) levels of dsDNA can be readily detected following short accumulation periods, with detection limits of 21 ppb and 200 ppb for oligo(dG)21 and dsDNA, respectively. The confined DNA layer demonstrated to be stable in air, in 0.200M acetate buffer pH 5.00, and in 0.020M phosphate buffer pH 7.40+0.50M NaCl.
Fil: Pedano, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the study of the adsorption and electrooxidation of nucleic acids on glassy carbon paste electrodes (GCPE) by using chronopotentiometric stripping analysis. The influence of electrochemical pretreatments, binder content, supporting electrolyte, accumulation potential, and time on the adsorption and further electrooxidation of oligo(dG)21 is discussed. The adsorption behavior of double stranded calf thymus DNA (dsDNA) on GCPE is also evaluated. Trace (ppb) levels of oligo(dG)21 and (ppm) levels of dsDNA can be readily detected following short accumulation periods, with detection limits of 21 ppb and 200 ppb for oligo(dG)21 and dsDNA, respectively. The confined DNA layer demonstrated to be stable in air, in 0.200M acetate buffer pH 5.00, and in 0.020M phosphate buffer pH 7.40+0.50M NaCl.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71302
Pedano, Maria Laura; Rivas, Gustavo Adolfo; Adsorption and electrooxidation of DNA at glassy carbon paste electrodes; Taylor ; Analytical Letters; 43; 10-11; 7-2010; 1703-1712
0003-2719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71302
identifier_str_mv Pedano, Maria Laura; Rivas, Gustavo Adolfo; Adsorption and electrooxidation of DNA at glassy carbon paste electrodes; Taylor ; Analytical Letters; 43; 10-11; 7-2010; 1703-1712
0003-2719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/00032711003653866
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032711003653866
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor
publisher.none.fl_str_mv Taylor
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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