Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress

Autores
Carassay, Luciano Raul; Bustos, Dolores Angela; Golberg, Alberto Daniel; Taleisnik, Edith
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tipburn in lettuce is a physiological disorder expressed as a necrosis in the margins of young developing leaves and is commonly observed under saline conditions. Tipburn is usually attributed to Ca 2+ deficiencies, and there has very limited research on other mechanisms that may contribute to tipburn development. This work examines whether symptoms are mediated by increased reactive oxygen species (ROS) production.Two butter lettuce (Lactuca sativa L.) varieties, Sunstar (Su) and Pontina (Po), with contrasting tipburn susceptibility were grown in hydroponics with low Ca 2+ (0.5mM), and with or without 50mM NaCl. Tipburn symptoms were observed only in Su, and only in the saline treatment. Tipburn incidence in response to topical treatments with Ca 2+ scavengers, Ca 2+ transport inhibitors, and antioxidants was assessed. All treatments were applied before symptom expression, and evaluated later, when symptoms were expected to occur. Superoxide presence in tissues was determined with nitro blue tetrazolium (NBT) and oxidative damage as malondialdehyde (MDA) content. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities were assayed.Under control and saline conditions, tipburn could be induced in both varieties by topical treatments with a Ca 2+ scavenger (EGTA) and Ca 2+ transport inhibitors (verapamil, LaCl 3) and reduced by supplying Ca 2+ along with a ionophore (A 23187). Tipburn symptoms were associated with locally produced ROS. O 2 - and oxidative damage significantly increased in leaf margins before symptom expression, while topical antioxidant applications (Tiron, DPI) reduced symptoms in treated leaves, but not in the rest of the plant. Antioxidant enzyme activity was higher in Po, and increased more in response to EGTA treatments, and may contribute to mitigating oxidative damage and tipburn expression in this variety.
Fil: Carassay, Luciano Raul. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golberg, Alberto Daniel. Universidad Nacional de La Pampa; Argentina
Fil: Taleisnik, Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Materia
CALCIUM
LACTUCA SATIVA L.
REACTIVE OXYGEN SPECIES
SALT STRESS
TIPBURN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189088
Acceder
id CONICETDig_67b607cacaee933388ef7e87c2d54913
oai_identifier_str oai:ri.conicet.gov.ar:11336/189088
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stressCarassay, Luciano RaulBustos, Dolores AngelaGolberg, Alberto DanielTaleisnik, EdithCALCIUMLACTUCA SATIVA L.REACTIVE OXYGEN SPECIESSALT STRESSTIPBURNhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Tipburn in lettuce is a physiological disorder expressed as a necrosis in the margins of young developing leaves and is commonly observed under saline conditions. Tipburn is usually attributed to Ca 2+ deficiencies, and there has very limited research on other mechanisms that may contribute to tipburn development. This work examines whether symptoms are mediated by increased reactive oxygen species (ROS) production.Two butter lettuce (Lactuca sativa L.) varieties, Sunstar (Su) and Pontina (Po), with contrasting tipburn susceptibility were grown in hydroponics with low Ca 2+ (0.5mM), and with or without 50mM NaCl. Tipburn symptoms were observed only in Su, and only in the saline treatment. Tipburn incidence in response to topical treatments with Ca 2+ scavengers, Ca 2+ transport inhibitors, and antioxidants was assessed. All treatments were applied before symptom expression, and evaluated later, when symptoms were expected to occur. Superoxide presence in tissues was determined with nitro blue tetrazolium (NBT) and oxidative damage as malondialdehyde (MDA) content. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities were assayed.Under control and saline conditions, tipburn could be induced in both varieties by topical treatments with a Ca 2+ scavenger (EGTA) and Ca 2+ transport inhibitors (verapamil, LaCl 3) and reduced by supplying Ca 2+ along with a ionophore (A 23187). Tipburn symptoms were associated with locally produced ROS. O 2 - and oxidative damage significantly increased in leaf margins before symptom expression, while topical antioxidant applications (Tiron, DPI) reduced symptoms in treated leaves, but not in the rest of the plant. Antioxidant enzyme activity was higher in Po, and increased more in response to EGTA treatments, and may contribute to mitigating oxidative damage and tipburn expression in this variety.Fil: Carassay, Luciano Raul. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; ArgentinaFil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golberg, Alberto Daniel. Universidad Nacional de La Pampa; ArgentinaFil: Taleisnik, Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaElsevier Gmbh2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189088Carassay, Luciano Raul; Bustos, Dolores Angela; Golberg, Alberto Daniel; Taleisnik, Edith; Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress; Elsevier Gmbh; Journal of Plant Physiology; 169; 3; 2-2012; 285-2930176-1617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0176161711004421info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2011.10.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:29:41Zoai:ri.conicet.gov.ar:11336/189088instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:29:42.096CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
title Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
spellingShingle Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
Carassay, Luciano Raul
CALCIUM
LACTUCA SATIVA L.
REACTIVE OXYGEN SPECIES
SALT STRESS
TIPBURN
title_short Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
title_full Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
title_fullStr Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
title_full_unstemmed Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
title_sort Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress
dc.creator.none.fl_str_mv Carassay, Luciano Raul
Bustos, Dolores Angela
Golberg, Alberto Daniel
Taleisnik, Edith
author Carassay, Luciano Raul
author_facet Carassay, Luciano Raul
Bustos, Dolores Angela
Golberg, Alberto Daniel
Taleisnik, Edith
author_role author
author2 Bustos, Dolores Angela
Golberg, Alberto Daniel
Taleisnik, Edith
author2_role author
author
author
dc.subject.none.fl_str_mv CALCIUM
LACTUCA SATIVA L.
REACTIVE OXYGEN SPECIES
SALT STRESS
TIPBURN
topic CALCIUM
LACTUCA SATIVA L.
REACTIVE OXYGEN SPECIES
SALT STRESS
TIPBURN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Tipburn in lettuce is a physiological disorder expressed as a necrosis in the margins of young developing leaves and is commonly observed under saline conditions. Tipburn is usually attributed to Ca 2+ deficiencies, and there has very limited research on other mechanisms that may contribute to tipburn development. This work examines whether symptoms are mediated by increased reactive oxygen species (ROS) production.Two butter lettuce (Lactuca sativa L.) varieties, Sunstar (Su) and Pontina (Po), with contrasting tipburn susceptibility were grown in hydroponics with low Ca 2+ (0.5mM), and with or without 50mM NaCl. Tipburn symptoms were observed only in Su, and only in the saline treatment. Tipburn incidence in response to topical treatments with Ca 2+ scavengers, Ca 2+ transport inhibitors, and antioxidants was assessed. All treatments were applied before symptom expression, and evaluated later, when symptoms were expected to occur. Superoxide presence in tissues was determined with nitro blue tetrazolium (NBT) and oxidative damage as malondialdehyde (MDA) content. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities were assayed.Under control and saline conditions, tipburn could be induced in both varieties by topical treatments with a Ca 2+ scavenger (EGTA) and Ca 2+ transport inhibitors (verapamil, LaCl 3) and reduced by supplying Ca 2+ along with a ionophore (A 23187). Tipburn symptoms were associated with locally produced ROS. O 2 - and oxidative damage significantly increased in leaf margins before symptom expression, while topical antioxidant applications (Tiron, DPI) reduced symptoms in treated leaves, but not in the rest of the plant. Antioxidant enzyme activity was higher in Po, and increased more in response to EGTA treatments, and may contribute to mitigating oxidative damage and tipburn expression in this variety.
Fil: Carassay, Luciano Raul. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golberg, Alberto Daniel. Universidad Nacional de La Pampa; Argentina
Fil: Taleisnik, Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
description Tipburn in lettuce is a physiological disorder expressed as a necrosis in the margins of young developing leaves and is commonly observed under saline conditions. Tipburn is usually attributed to Ca 2+ deficiencies, and there has very limited research on other mechanisms that may contribute to tipburn development. This work examines whether symptoms are mediated by increased reactive oxygen species (ROS) production.Two butter lettuce (Lactuca sativa L.) varieties, Sunstar (Su) and Pontina (Po), with contrasting tipburn susceptibility were grown in hydroponics with low Ca 2+ (0.5mM), and with or without 50mM NaCl. Tipburn symptoms were observed only in Su, and only in the saline treatment. Tipburn incidence in response to topical treatments with Ca 2+ scavengers, Ca 2+ transport inhibitors, and antioxidants was assessed. All treatments were applied before symptom expression, and evaluated later, when symptoms were expected to occur. Superoxide presence in tissues was determined with nitro blue tetrazolium (NBT) and oxidative damage as malondialdehyde (MDA) content. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities were assayed.Under control and saline conditions, tipburn could be induced in both varieties by topical treatments with a Ca 2+ scavenger (EGTA) and Ca 2+ transport inhibitors (verapamil, LaCl 3) and reduced by supplying Ca 2+ along with a ionophore (A 23187). Tipburn symptoms were associated with locally produced ROS. O 2 - and oxidative damage significantly increased in leaf margins before symptom expression, while topical antioxidant applications (Tiron, DPI) reduced symptoms in treated leaves, but not in the rest of the plant. Antioxidant enzyme activity was higher in Po, and increased more in response to EGTA treatments, and may contribute to mitigating oxidative damage and tipburn expression in this variety.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189088
Carassay, Luciano Raul; Bustos, Dolores Angela; Golberg, Alberto Daniel; Taleisnik, Edith; Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress; Elsevier Gmbh; Journal of Plant Physiology; 169; 3; 2-2012; 285-293
0176-1617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189088
identifier_str_mv Carassay, Luciano Raul; Bustos, Dolores Angela; Golberg, Alberto Daniel; Taleisnik, Edith; Tipburn in salt-affected lettuce (Lactuca sativa L.) plants results from local oxidative stress; Elsevier Gmbh; Journal of Plant Physiology; 169; 3; 2-2012; 285-293
0176-1617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0176161711004421
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2011.10.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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