UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables

Autores
Fusari Gómez, Cecilia Mara; Locatelli, Daniela Ana; Altamirano, Jorgelina Cecilia; Camargo, Alejandra Beatriz
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Total isothiocyanates content (ITC) is considered a good indicator of bioactive compounds responsible for beneficial effects related to Brassicaceae vegetables. Analytical performance is a critical factor for routine analysis in plant tissues.The extraction technique for isolating phytochemicals fromBrassicaceae vegetables is currently the bottleneck of themethodology. The aimof this work was to optimize this step in the analytical process. Fast and less expensive alternative, based on ultrasound-assisted extraction technique (UAE) for direct extraction of GLS into an aqueous phase and further analysis of the hydrolysis product, was optimized. Full factorial (2) design followed by Central Composite Design (CCD) was used to obtain the optimum extraction conditions. Selected conditions were homogenization time (9 min); ultrasound bath time (5 min); and sample-to-solvent ratio (1 : 5 w/vmgmL−1). Theproposed analytical methodology exhibits satisfactory analytical performance in terms of linearity, precision (RSD < 2.4%), and limits of detection (26 nmol g−1 w.w.).Thenew analyticalmethodology was applied to cauliflower, cabbage, watercress, and broccoli samples with recoveries higher than 86%.TheUAE extraction technique was showed to be efficient for real samples analysis leading to sensible, selective, and reproducible methodology for ITC analysis.
Fil: Fusari Gómez, Cecilia Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Locatelli, Daniela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
ISOTHIOCYANATES
BRASSICACEAE
UAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104417
Acceder
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network_name_str CONICET Digital (CONICET)
spelling UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetablesFusari Gómez, Cecilia MaraLocatelli, Daniela AnaAltamirano, Jorgelina CeciliaCamargo, Alejandra BeatrizISOTHIOCYANATESBRASSICACEAEUAEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Total isothiocyanates content (ITC) is considered a good indicator of bioactive compounds responsible for beneficial effects related to Brassicaceae vegetables. Analytical performance is a critical factor for routine analysis in plant tissues.The extraction technique for isolating phytochemicals fromBrassicaceae vegetables is currently the bottleneck of themethodology. The aimof this work was to optimize this step in the analytical process. Fast and less expensive alternative, based on ultrasound-assisted extraction technique (UAE) for direct extraction of GLS into an aqueous phase and further analysis of the hydrolysis product, was optimized. Full factorial (2) design followed by Central Composite Design (CCD) was used to obtain the optimum extraction conditions. Selected conditions were homogenization time (9 min); ultrasound bath time (5 min); and sample-to-solvent ratio (1 : 5 w/vmgmL−1). Theproposed analytical methodology exhibits satisfactory analytical performance in terms of linearity, precision (RSD < 2.4%), and limits of detection (26 nmol g−1 w.w.).Thenew analyticalmethodology was applied to cauliflower, cabbage, watercress, and broccoli samples with recoveries higher than 86%.TheUAE extraction technique was showed to be efficient for real samples analysis leading to sensible, selective, and reproducible methodology for ITC analysis.Fil: Fusari Gómez, Cecilia Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Locatelli, Daniela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaHindawi Publishing Corporation2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104417Fusari Gómez, Cecilia Mara; Locatelli, Daniela Ana; Altamirano, Jorgelina Cecilia; Camargo, Alejandra Beatriz; UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables; Hindawi Publishing Corporation; Journal of Chemistry; 2015; 2946; 11-2015; 1-82090-9063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/jchem/2015/294601/info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/294601info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:43Zoai:ri.conicet.gov.ar:11336/104417instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:43.363CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
title UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
spellingShingle UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
Fusari Gómez, Cecilia Mara
ISOTHIOCYANATES
BRASSICACEAE
UAE
title_short UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
title_full UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
title_fullStr UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
title_full_unstemmed UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
title_sort UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables
dc.creator.none.fl_str_mv Fusari Gómez, Cecilia Mara
Locatelli, Daniela Ana
Altamirano, Jorgelina Cecilia
Camargo, Alejandra Beatriz
author Fusari Gómez, Cecilia Mara
author_facet Fusari Gómez, Cecilia Mara
Locatelli, Daniela Ana
Altamirano, Jorgelina Cecilia
Camargo, Alejandra Beatriz
author_role author
author2 Locatelli, Daniela Ana
Altamirano, Jorgelina Cecilia
Camargo, Alejandra Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv ISOTHIOCYANATES
BRASSICACEAE
UAE
topic ISOTHIOCYANATES
BRASSICACEAE
UAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Total isothiocyanates content (ITC) is considered a good indicator of bioactive compounds responsible for beneficial effects related to Brassicaceae vegetables. Analytical performance is a critical factor for routine analysis in plant tissues.The extraction technique for isolating phytochemicals fromBrassicaceae vegetables is currently the bottleneck of themethodology. The aimof this work was to optimize this step in the analytical process. Fast and less expensive alternative, based on ultrasound-assisted extraction technique (UAE) for direct extraction of GLS into an aqueous phase and further analysis of the hydrolysis product, was optimized. Full factorial (2) design followed by Central Composite Design (CCD) was used to obtain the optimum extraction conditions. Selected conditions were homogenization time (9 min); ultrasound bath time (5 min); and sample-to-solvent ratio (1 : 5 w/vmgmL−1). Theproposed analytical methodology exhibits satisfactory analytical performance in terms of linearity, precision (RSD < 2.4%), and limits of detection (26 nmol g−1 w.w.).Thenew analyticalmethodology was applied to cauliflower, cabbage, watercress, and broccoli samples with recoveries higher than 86%.TheUAE extraction technique was showed to be efficient for real samples analysis leading to sensible, selective, and reproducible methodology for ITC analysis.
Fil: Fusari Gómez, Cecilia Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Locatelli, Daniela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Total isothiocyanates content (ITC) is considered a good indicator of bioactive compounds responsible for beneficial effects related to Brassicaceae vegetables. Analytical performance is a critical factor for routine analysis in plant tissues.The extraction technique for isolating phytochemicals fromBrassicaceae vegetables is currently the bottleneck of themethodology. The aimof this work was to optimize this step in the analytical process. Fast and less expensive alternative, based on ultrasound-assisted extraction technique (UAE) for direct extraction of GLS into an aqueous phase and further analysis of the hydrolysis product, was optimized. Full factorial (2) design followed by Central Composite Design (CCD) was used to obtain the optimum extraction conditions. Selected conditions were homogenization time (9 min); ultrasound bath time (5 min); and sample-to-solvent ratio (1 : 5 w/vmgmL−1). Theproposed analytical methodology exhibits satisfactory analytical performance in terms of linearity, precision (RSD < 2.4%), and limits of detection (26 nmol g−1 w.w.).Thenew analyticalmethodology was applied to cauliflower, cabbage, watercress, and broccoli samples with recoveries higher than 86%.TheUAE extraction technique was showed to be efficient for real samples analysis leading to sensible, selective, and reproducible methodology for ITC analysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104417
Fusari Gómez, Cecilia Mara; Locatelli, Daniela Ana; Altamirano, Jorgelina Cecilia; Camargo, Alejandra Beatriz; UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables; Hindawi Publishing Corporation; Journal of Chemistry; 2015; 2946; 11-2015; 1-8
2090-9063
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104417
identifier_str_mv Fusari Gómez, Cecilia Mara; Locatelli, Daniela Ana; Altamirano, Jorgelina Cecilia; Camargo, Alejandra Beatriz; UAE-HPLC-UV: new contribution for fast determination of total isothiocyanates in Brassicaceae vegetables; Hindawi Publishing Corporation; Journal of Chemistry; 2015; 2946; 11-2015; 1-8
2090-9063
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/jchem/2015/294601/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/294601
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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