Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis
- Autores
- Esteve Bruna, David; Carrasco López, Cristian; Blanco Touriñán, Noel; Iserte, Javier Alonso; Calleja Cabrera, Julián; Perea Resa, Carlos; Úrbez, Cristina; Carrasco, Pedro; Yanovsky, Marcelo Javier; Blázquez, Miguel A.; Salinas, Julio; Alabadí, David
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Although originally identified as the components of the complex aiding the cytosolic chaperonin CCT in the folding of actins and tubulins in the cytosol, prefoldins (PFDs) are emerging as novel regulators influencing gene expression in the nucleus. Work conducted mainly in yeast and animals showed that PFDs act as transcriptional regulators and participate in the nuclear proteostasis. To investigate new functions of PFDs, we performed a co-expression analysis in Arabidopsis thaliana. Results revealed co-expression between PFD and the Sm-like (LSM) genes, which encode the LSM2–8 spliceosome core complex, in this model organism. Here, we show that PFDs interact with and are required to maintain adequate levels of the LSM2–8 complex. Our data indicate that levels of the LSM8 protein, which defines and confers the functional specificity of the complex, are reduced in pfd mutants and in response to the Hsp90 inhibitor geldanamycin. We provide biochemical evidence showing that LSM8 is a client of Hsp90 and that PFD4 mediates the interaction between both proteins. Consistent with our results and with the role of the LSM2–8 complex in splicing through the stabilization of the U6 snRNA, pfd mutants showed reduced levels of this snRNA and altered pre-mRNA splicing patterns.
Fil: Esteve Bruna, David. Universidad Politécnica de Valencia; España
Fil: Carrasco López, Cristian. Consejo Superior de Investigaciones Científicas; España
Fil: Blanco Touriñán, Noel. Universidad Politécnica de Valencia; España
Fil: Iserte, Javier Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Calleja Cabrera, Julián. Universidad Politécnica de Valencia; España
Fil: Perea Resa, Carlos. Consejo Superior de Investigaciones Científicas; España
Fil: Úrbez, Cristina. Universidad Politécnica de Valencia; España
Fil: Carrasco, Pedro. Universidad Politécnica de Valencia; España
Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Blázquez, Miguel A.. Universidad Politécnica de Valencia; España
Fil: Salinas, Julio. Consejo Superior de Investigaciones Científicas; España
Fil: Alabadí, David. Universidad Politécnica de Valencia; España - Materia
-
LSM2-8 complex
prefoldins
spliceosome - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/138711
Ver los metadatos del registro completo
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Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in ArabidopsisEsteve Bruna, DavidCarrasco López, CristianBlanco Touriñán, NoelIserte, Javier AlonsoCalleja Cabrera, JuliánPerea Resa, CarlosÚrbez, CristinaCarrasco, PedroYanovsky, Marcelo JavierBlázquez, Miguel A.Salinas, JulioAlabadí, DavidLSM2-8 complexprefoldinsspliceosomehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although originally identified as the components of the complex aiding the cytosolic chaperonin CCT in the folding of actins and tubulins in the cytosol, prefoldins (PFDs) are emerging as novel regulators influencing gene expression in the nucleus. Work conducted mainly in yeast and animals showed that PFDs act as transcriptional regulators and participate in the nuclear proteostasis. To investigate new functions of PFDs, we performed a co-expression analysis in Arabidopsis thaliana. Results revealed co-expression between PFD and the Sm-like (LSM) genes, which encode the LSM2–8 spliceosome core complex, in this model organism. Here, we show that PFDs interact with and are required to maintain adequate levels of the LSM2–8 complex. Our data indicate that levels of the LSM8 protein, which defines and confers the functional specificity of the complex, are reduced in pfd mutants and in response to the Hsp90 inhibitor geldanamycin. We provide biochemical evidence showing that LSM8 is a client of Hsp90 and that PFD4 mediates the interaction between both proteins. Consistent with our results and with the role of the LSM2–8 complex in splicing through the stabilization of the U6 snRNA, pfd mutants showed reduced levels of this snRNA and altered pre-mRNA splicing patterns.Fil: Esteve Bruna, David. Universidad Politécnica de Valencia; EspañaFil: Carrasco López, Cristian. Consejo Superior de Investigaciones Científicas; EspañaFil: Blanco Touriñán, Noel. Universidad Politécnica de Valencia; EspañaFil: Iserte, Javier Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Calleja Cabrera, Julián. Universidad Politécnica de Valencia; EspañaFil: Perea Resa, Carlos. Consejo Superior de Investigaciones Científicas; EspañaFil: Úrbez, Cristina. Universidad Politécnica de Valencia; EspañaFil: Carrasco, Pedro. Universidad Politécnica de Valencia; EspañaFil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Blázquez, Miguel A.. Universidad Politécnica de Valencia; EspañaFil: Salinas, Julio. Consejo Superior de Investigaciones Científicas; EspañaFil: Alabadí, David. Universidad Politécnica de Valencia; EspañaOxford University Press2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138711Esteve Bruna, David; Carrasco López, Cristian; Blanco Touriñán, Noel; Iserte, Javier Alonso; Calleja Cabrera, Julián; et al.; Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis; Oxford University Press; Nucleic Acids Research; 48; 11; 6-2020; 6280-62930305-10481362-4962CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/48/11/6280/5836189info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkaa354info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:13Zoai:ri.conicet.gov.ar:11336/138711instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:13.676CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
title |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
spellingShingle |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis Esteve Bruna, David LSM2-8 complex prefoldins spliceosome |
title_short |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
title_full |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
title_fullStr |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
title_full_unstemmed |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
title_sort |
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis |
dc.creator.none.fl_str_mv |
Esteve Bruna, David Carrasco López, Cristian Blanco Touriñán, Noel Iserte, Javier Alonso Calleja Cabrera, Julián Perea Resa, Carlos Úrbez, Cristina Carrasco, Pedro Yanovsky, Marcelo Javier Blázquez, Miguel A. Salinas, Julio Alabadí, David |
author |
Esteve Bruna, David |
author_facet |
Esteve Bruna, David Carrasco López, Cristian Blanco Touriñán, Noel Iserte, Javier Alonso Calleja Cabrera, Julián Perea Resa, Carlos Úrbez, Cristina Carrasco, Pedro Yanovsky, Marcelo Javier Blázquez, Miguel A. Salinas, Julio Alabadí, David |
author_role |
author |
author2 |
Carrasco López, Cristian Blanco Touriñán, Noel Iserte, Javier Alonso Calleja Cabrera, Julián Perea Resa, Carlos Úrbez, Cristina Carrasco, Pedro Yanovsky, Marcelo Javier Blázquez, Miguel A. Salinas, Julio Alabadí, David |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
LSM2-8 complex prefoldins spliceosome |
topic |
LSM2-8 complex prefoldins spliceosome |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Although originally identified as the components of the complex aiding the cytosolic chaperonin CCT in the folding of actins and tubulins in the cytosol, prefoldins (PFDs) are emerging as novel regulators influencing gene expression in the nucleus. Work conducted mainly in yeast and animals showed that PFDs act as transcriptional regulators and participate in the nuclear proteostasis. To investigate new functions of PFDs, we performed a co-expression analysis in Arabidopsis thaliana. Results revealed co-expression between PFD and the Sm-like (LSM) genes, which encode the LSM2–8 spliceosome core complex, in this model organism. Here, we show that PFDs interact with and are required to maintain adequate levels of the LSM2–8 complex. Our data indicate that levels of the LSM8 protein, which defines and confers the functional specificity of the complex, are reduced in pfd mutants and in response to the Hsp90 inhibitor geldanamycin. We provide biochemical evidence showing that LSM8 is a client of Hsp90 and that PFD4 mediates the interaction between both proteins. Consistent with our results and with the role of the LSM2–8 complex in splicing through the stabilization of the U6 snRNA, pfd mutants showed reduced levels of this snRNA and altered pre-mRNA splicing patterns. Fil: Esteve Bruna, David. Universidad Politécnica de Valencia; España Fil: Carrasco López, Cristian. Consejo Superior de Investigaciones Científicas; España Fil: Blanco Touriñán, Noel. Universidad Politécnica de Valencia; España Fil: Iserte, Javier Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Calleja Cabrera, Julián. Universidad Politécnica de Valencia; España Fil: Perea Resa, Carlos. Consejo Superior de Investigaciones Científicas; España Fil: Úrbez, Cristina. Universidad Politécnica de Valencia; España Fil: Carrasco, Pedro. Universidad Politécnica de Valencia; España Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Blázquez, Miguel A.. Universidad Politécnica de Valencia; España Fil: Salinas, Julio. Consejo Superior de Investigaciones Científicas; España Fil: Alabadí, David. Universidad Politécnica de Valencia; España |
description |
Although originally identified as the components of the complex aiding the cytosolic chaperonin CCT in the folding of actins and tubulins in the cytosol, prefoldins (PFDs) are emerging as novel regulators influencing gene expression in the nucleus. Work conducted mainly in yeast and animals showed that PFDs act as transcriptional regulators and participate in the nuclear proteostasis. To investigate new functions of PFDs, we performed a co-expression analysis in Arabidopsis thaliana. Results revealed co-expression between PFD and the Sm-like (LSM) genes, which encode the LSM2–8 spliceosome core complex, in this model organism. Here, we show that PFDs interact with and are required to maintain adequate levels of the LSM2–8 complex. Our data indicate that levels of the LSM8 protein, which defines and confers the functional specificity of the complex, are reduced in pfd mutants and in response to the Hsp90 inhibitor geldanamycin. We provide biochemical evidence showing that LSM8 is a client of Hsp90 and that PFD4 mediates the interaction between both proteins. Consistent with our results and with the role of the LSM2–8 complex in splicing through the stabilization of the U6 snRNA, pfd mutants showed reduced levels of this snRNA and altered pre-mRNA splicing patterns. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/138711 Esteve Bruna, David; Carrasco López, Cristian; Blanco Touriñán, Noel; Iserte, Javier Alonso; Calleja Cabrera, Julián; et al.; Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis; Oxford University Press; Nucleic Acids Research; 48; 11; 6-2020; 6280-6293 0305-1048 1362-4962 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/138711 |
identifier_str_mv |
Esteve Bruna, David; Carrasco López, Cristian; Blanco Touriñán, Noel; Iserte, Javier Alonso; Calleja Cabrera, Julián; et al.; Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis; Oxford University Press; Nucleic Acids Research; 48; 11; 6-2020; 6280-6293 0305-1048 1362-4962 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/48/11/6280/5836189 info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkaa354 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613648765419520 |
score |
13.070432 |