The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms

Autores
Schlaen, Rubén Gustavo; Mancini, Estefania; Sanchez, Sabrina Elena; Perez Santangelo, Maria Soledad; Rugnone, Matias Leandro; Simpson, Craig G.; Brown, John W. S.; Zhang, Xu; Chernomoretz, Ariel; Yanovsky, Marcelo Javier
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
Fil: Schlaen, Rubén Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Mancini, Estefania. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Sanchez, Sabrina Elena. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Perez Santangelo, Maria Soledad. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Rugnone, Matias Leandro. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Simpson, Craig G.. James Hutton Institute; Reino Unido
Fil: Brown, John W. S.. James Hutton Institute; Reino Unido. University of Dundee at James Hutton Institute; Reino Unido
Fil: Zhang, Xu. University of Illinois at Chicago; Estados Unidos
Fil: Chernomoretz, Ariel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Materia
Arabidopsis Arabidopsis
Circadian Rhythms
Alternative Splicing
Spliceosome Assembly
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10316
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10316
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythmsSchlaen, Rubén GustavoMancini, EstefaniaSanchez, Sabrina ElenaPerez Santangelo, Maria SoledadRugnone, Matias LeandroSimpson, Craig G.Brown, John W. S.Zhang, XuChernomoretz, ArielYanovsky, Marcelo JavierArabidopsis ArabidopsisCircadian RhythmsAlternative SplicingSpliceosome Assemblyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.Fil: Schlaen, Rubén Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Mancini, Estefania. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Sanchez, Sabrina Elena. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Perez Santangelo, Maria Soledad. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Rugnone, Matias Leandro. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Simpson, Craig G.. James Hutton Institute; Reino UnidoFil: Brown, John W. S.. James Hutton Institute; Reino Unido. University of Dundee at James Hutton Institute; Reino UnidoFil: Zhang, Xu. University of Illinois at Chicago; Estados UnidosFil: Chernomoretz, Ariel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaNational Academy Of Sciences2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10316Schlaen, Rubén Gustavo; Mancini, Estefania; Sanchez, Sabrina Elena; Perez Santangelo, Maria Soledad; Rugnone, Matias Leandro; et al.; The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 112; 30; 7-2015; 9382-93870027-8424enginfo:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/112/30/9382info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1504541112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:18:53Zoai:ri.conicet.gov.ar:11336/10316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:18:53.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
title The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
spellingShingle The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
Schlaen, Rubén Gustavo
Arabidopsis Arabidopsis
Circadian Rhythms
Alternative Splicing
Spliceosome Assembly
title_short The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
title_full The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
title_fullStr The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
title_full_unstemmed The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
title_sort The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms
dc.creator.none.fl_str_mv Schlaen, Rubén Gustavo
Mancini, Estefania
Sanchez, Sabrina Elena
Perez Santangelo, Maria Soledad
Rugnone, Matias Leandro
Simpson, Craig G.
Brown, John W. S.
Zhang, Xu
Chernomoretz, Ariel
Yanovsky, Marcelo Javier
author Schlaen, Rubén Gustavo
author_facet Schlaen, Rubén Gustavo
Mancini, Estefania
Sanchez, Sabrina Elena
Perez Santangelo, Maria Soledad
Rugnone, Matias Leandro
Simpson, Craig G.
Brown, John W. S.
Zhang, Xu
Chernomoretz, Ariel
Yanovsky, Marcelo Javier
author_role author
author2 Mancini, Estefania
Sanchez, Sabrina Elena
Perez Santangelo, Maria Soledad
Rugnone, Matias Leandro
Simpson, Craig G.
Brown, John W. S.
Zhang, Xu
Chernomoretz, Ariel
Yanovsky, Marcelo Javier
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Arabidopsis
Circadian Rhythms
Alternative Splicing
Spliceosome Assembly
topic Arabidopsis Arabidopsis
Circadian Rhythms
Alternative Splicing
Spliceosome Assembly
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
Fil: Schlaen, Rubén Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Mancini, Estefania. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Sanchez, Sabrina Elena. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Perez Santangelo, Maria Soledad. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Rugnone, Matias Leandro. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Simpson, Craig G.. James Hutton Institute; Reino Unido
Fil: Brown, John W. S.. James Hutton Institute; Reino Unido. University of Dundee at James Hutton Institute; Reino Unido
Fil: Zhang, Xu. University of Illinois at Chicago; Estados Unidos
Fil: Chernomoretz, Ariel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
description The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10316
Schlaen, Rubén Gustavo; Mancini, Estefania; Sanchez, Sabrina Elena; Perez Santangelo, Maria Soledad; Rugnone, Matias Leandro; et al.; The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 112; 30; 7-2015; 9382-9387
0027-8424
url http://hdl.handle.net/11336/10316
identifier_str_mv Schlaen, Rubén Gustavo; Mancini, Estefania; Sanchez, Sabrina Elena; Perez Santangelo, Maria Soledad; Rugnone, Matias Leandro; et al.; The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 112; 30; 7-2015; 9382-9387
0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/112/30/9382
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1504541112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy Of Sciences
publisher.none.fl_str_mv National Academy Of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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