Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitu...

Autores
Viver, Tomeu; Orellana, Luis; González Torres, Pedro; Díaz, Sara; Urdiain, Mercedes; Farias, Maria Eugenia; Benes, Vladimir; Kaempfer, Peter; Shahinpei, Azadeh; Ali Amoozegar, Mohammad; Amann, Rudolf; Antón, Josefa; Konstantinidis, Konstantinos T.; Rosselló Móra, Ramon
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The application of tandem MALDI-TOF MS screening with 16S rRNA gene sequencing of selected isolates has been demonstrated to be an excellent approach for retrieving novelty from large-scale culturing. The application of such methodologies in different hypersaline samples allowed the isolation of the culture-recalcitrant Salinibacter ruber second phylotype (EHB-2) for the first time, as well as a new species recently isolated from the Argentinian Altiplano hypersaline lakes. In this study, the genome sequences of the different species of the phylum Rhodothermaeota were compared and the genetic repertoire along the evolutionary gradient was analyzed together with each intraspecific variability. Altogether, the results indicated an open pan-genome for the family Salinibacteraceae, as well as the codification of relevant traits such as diverse rhodopsin genes, CRISPR-Cas systems and spacers, and one T6SS secretion system that could give ecological advantages to an EHB-2 isolate. For the new Salinibacter species, we propose the name Salinibacter altiplanensis sp. nov. (the designated type strain is AN15T = CECT 9105T = IBRC-M 11031T).
Fil: Viver, Tomeu. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Orellana, Luis. Instituto Tecnológico de Georgia; Estados Unidos. Georgia Institute of Techology; Estados Unidos
Fil: González Torres, Pedro. Universidad de Alicante; España
Fil: Díaz, Sara. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Urdiain, Mercedes. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benes, Vladimir. European Molecular Biology Laboratory Heidelberg;
Fil: Kaempfer, Peter. Justus Liebig University Giessen;
Fil: Shahinpei, Azadeh. University Of Tehran; Irán
Fil: Ali Amoozegar, Mohammad. University Of Tehran; Irán
Fil: Amann, Rudolf. Max Planck Institut Für Marine Mikrobiologie;
Fil: Antón, Josefa. Universidad de Alicante; España
Fil: Konstantinidis, Konstantinos T.. Georgia Institute of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados Unidos
Fil: Rosselló Móra, Ramon. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Materia
GENOMIC
HALOPHILES
MALDI-TOF MS
SALINIBACTER RUBER
SALT LAKE
SALTERNS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82328
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82328
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian AltiplanoViver, TomeuOrellana, LuisGonzález Torres, PedroDíaz, SaraUrdiain, MercedesFarias, Maria EugeniaBenes, VladimirKaempfer, PeterShahinpei, AzadehAli Amoozegar, MohammadAmann, RudolfAntón, JosefaKonstantinidis, Konstantinos T.Rosselló Móra, RamonGENOMICHALOPHILESMALDI-TOF MSSALINIBACTER RUBERSALT LAKESALTERNShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The application of tandem MALDI-TOF MS screening with 16S rRNA gene sequencing of selected isolates has been demonstrated to be an excellent approach for retrieving novelty from large-scale culturing. The application of such methodologies in different hypersaline samples allowed the isolation of the culture-recalcitrant Salinibacter ruber second phylotype (EHB-2) for the first time, as well as a new species recently isolated from the Argentinian Altiplano hypersaline lakes. In this study, the genome sequences of the different species of the phylum Rhodothermaeota were compared and the genetic repertoire along the evolutionary gradient was analyzed together with each intraspecific variability. Altogether, the results indicated an open pan-genome for the family Salinibacteraceae, as well as the codification of relevant traits such as diverse rhodopsin genes, CRISPR-Cas systems and spacers, and one T6SS secretion system that could give ecological advantages to an EHB-2 isolate. For the new Salinibacter species, we propose the name Salinibacter altiplanensis sp. nov. (the designated type strain is AN15T = CECT 9105T = IBRC-M 11031T).Fil: Viver, Tomeu. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; EspañaFil: Orellana, Luis. Instituto Tecnológico de Georgia; Estados Unidos. Georgia Institute of Techology; Estados UnidosFil: González Torres, Pedro. Universidad de Alicante; EspañaFil: Díaz, Sara. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; EspañaFil: Urdiain, Mercedes. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; EspañaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Benes, Vladimir. European Molecular Biology Laboratory Heidelberg;Fil: Kaempfer, Peter. Justus Liebig University Giessen;Fil: Shahinpei, Azadeh. University Of Tehran; IránFil: Ali Amoozegar, Mohammad. University Of Tehran; IránFil: Amann, Rudolf. Max Planck Institut Für Marine Mikrobiologie;Fil: Antón, Josefa. Universidad de Alicante; EspañaFil: Konstantinidis, Konstantinos T.. Georgia Institute of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados UnidosFil: Rosselló Móra, Ramon. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; EspañaElsevier Gmbh2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82328Viver, Tomeu; Orellana, Luis; González Torres, Pedro; Díaz, Sara; Urdiain, Mercedes; et al.; Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano; Elsevier Gmbh; Systematic And Applied Microbiology; 41; 3; 5-2018; 198-2120723-2020CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.syapm.2017.12.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0723202018300250?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:04:07Zoai:ri.conicet.gov.ar:11336/82328instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:04:07.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
title Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
spellingShingle Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
Viver, Tomeu
GENOMIC
HALOPHILES
MALDI-TOF MS
SALINIBACTER RUBER
SALT LAKE
SALTERNS
title_short Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
title_full Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
title_fullStr Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
title_full_unstemmed Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
title_sort Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano
dc.creator.none.fl_str_mv Viver, Tomeu
Orellana, Luis
González Torres, Pedro
Díaz, Sara
Urdiain, Mercedes
Farias, Maria Eugenia
Benes, Vladimir
Kaempfer, Peter
Shahinpei, Azadeh
Ali Amoozegar, Mohammad
Amann, Rudolf
Antón, Josefa
Konstantinidis, Konstantinos T.
Rosselló Móra, Ramon
author Viver, Tomeu
author_facet Viver, Tomeu
Orellana, Luis
González Torres, Pedro
Díaz, Sara
Urdiain, Mercedes
Farias, Maria Eugenia
Benes, Vladimir
Kaempfer, Peter
Shahinpei, Azadeh
Ali Amoozegar, Mohammad
Amann, Rudolf
Antón, Josefa
Konstantinidis, Konstantinos T.
Rosselló Móra, Ramon
author_role author
author2 Orellana, Luis
González Torres, Pedro
Díaz, Sara
Urdiain, Mercedes
Farias, Maria Eugenia
Benes, Vladimir
Kaempfer, Peter
Shahinpei, Azadeh
Ali Amoozegar, Mohammad
Amann, Rudolf
Antón, Josefa
Konstantinidis, Konstantinos T.
Rosselló Móra, Ramon
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENOMIC
HALOPHILES
MALDI-TOF MS
SALINIBACTER RUBER
SALT LAKE
SALTERNS
topic GENOMIC
HALOPHILES
MALDI-TOF MS
SALINIBACTER RUBER
SALT LAKE
SALTERNS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The application of tandem MALDI-TOF MS screening with 16S rRNA gene sequencing of selected isolates has been demonstrated to be an excellent approach for retrieving novelty from large-scale culturing. The application of such methodologies in different hypersaline samples allowed the isolation of the culture-recalcitrant Salinibacter ruber second phylotype (EHB-2) for the first time, as well as a new species recently isolated from the Argentinian Altiplano hypersaline lakes. In this study, the genome sequences of the different species of the phylum Rhodothermaeota were compared and the genetic repertoire along the evolutionary gradient was analyzed together with each intraspecific variability. Altogether, the results indicated an open pan-genome for the family Salinibacteraceae, as well as the codification of relevant traits such as diverse rhodopsin genes, CRISPR-Cas systems and spacers, and one T6SS secretion system that could give ecological advantages to an EHB-2 isolate. For the new Salinibacter species, we propose the name Salinibacter altiplanensis sp. nov. (the designated type strain is AN15T = CECT 9105T = IBRC-M 11031T).
Fil: Viver, Tomeu. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Orellana, Luis. Instituto Tecnológico de Georgia; Estados Unidos. Georgia Institute of Techology; Estados Unidos
Fil: González Torres, Pedro. Universidad de Alicante; España
Fil: Díaz, Sara. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Urdiain, Mercedes. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benes, Vladimir. European Molecular Biology Laboratory Heidelberg;
Fil: Kaempfer, Peter. Justus Liebig University Giessen;
Fil: Shahinpei, Azadeh. University Of Tehran; Irán
Fil: Ali Amoozegar, Mohammad. University Of Tehran; Irán
Fil: Amann, Rudolf. Max Planck Institut Für Marine Mikrobiologie;
Fil: Antón, Josefa. Universidad de Alicante; España
Fil: Konstantinidis, Konstantinos T.. Georgia Institute of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados Unidos
Fil: Rosselló Móra, Ramon. Consejo Superior de Investigaciones Cientificas. Instituto Mediterraneo de Estudios Avanzados; España
description The application of tandem MALDI-TOF MS screening with 16S rRNA gene sequencing of selected isolates has been demonstrated to be an excellent approach for retrieving novelty from large-scale culturing. The application of such methodologies in different hypersaline samples allowed the isolation of the culture-recalcitrant Salinibacter ruber second phylotype (EHB-2) for the first time, as well as a new species recently isolated from the Argentinian Altiplano hypersaline lakes. In this study, the genome sequences of the different species of the phylum Rhodothermaeota were compared and the genetic repertoire along the evolutionary gradient was analyzed together with each intraspecific variability. Altogether, the results indicated an open pan-genome for the family Salinibacteraceae, as well as the codification of relevant traits such as diverse rhodopsin genes, CRISPR-Cas systems and spacers, and one T6SS secretion system that could give ecological advantages to an EHB-2 isolate. For the new Salinibacter species, we propose the name Salinibacter altiplanensis sp. nov. (the designated type strain is AN15T = CECT 9105T = IBRC-M 11031T).
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82328
Viver, Tomeu; Orellana, Luis; González Torres, Pedro; Díaz, Sara; Urdiain, Mercedes; et al.; Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano; Elsevier Gmbh; Systematic And Applied Microbiology; 41; 3; 5-2018; 198-212
0723-2020
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82328
identifier_str_mv Viver, Tomeu; Orellana, Luis; González Torres, Pedro; Díaz, Sara; Urdiain, Mercedes; et al.; Genomic comparison between members of the Salinibacteraceae family, and description of a new species of Salinibacter (Salinibacter altiplanensis sp. nov.) isolated from high altitude hypersaline environments of the Argentinian Altiplano; Elsevier Gmbh; Systematic And Applied Microbiology; 41; 3; 5-2018; 198-212
0723-2020
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.syapm.2017.12.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0723202018300250?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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