Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus

Autores
Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus.
Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Fil: Garcia, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Fil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Materia
HEMOCYANIN
MALDI-TOF
LIPIDS
FLUORESCENCE
ARACHNID
HEXAMER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9064
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9064
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricusLaino, AldanaGarcia, FernandoCunningham, Monica LilianaHEMOCYANINMALDI-TOFLIPIDSFLUORESCENCEARACHNIDHEXAMERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus.Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; ArgentinaFil: Garcia, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; ArgentinaFil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; ArgentinaInst Histol Embriol-conicet2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9064Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana; Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus; Inst Histol Embriol-conicet; Biocell; 39; 1; 6-2015; 33-400327-95451667-5746enginfo:eu-repo/semantics/altIdentifier/url/http://www.mendoza-conicet.gov.ar/biocell/vol/pdf/39_1/Biocell_MS4038_Cunningham.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:22Zoai:ri.conicet.gov.ar:11336/9064instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:22.909CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
title Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
spellingShingle Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
Laino, Aldana
HEMOCYANIN
MALDI-TOF
LIPIDS
FLUORESCENCE
ARACHNID
HEXAMER
title_short Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
title_full Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
title_fullStr Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
title_full_unstemmed Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
title_sort Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus
dc.creator.none.fl_str_mv Laino, Aldana
Garcia, Fernando
Cunningham, Monica Liliana
author Laino, Aldana
author_facet Laino, Aldana
Garcia, Fernando
Cunningham, Monica Liliana
author_role author
author2 Garcia, Fernando
Cunningham, Monica Liliana
author2_role author
author
dc.subject.none.fl_str_mv HEMOCYANIN
MALDI-TOF
LIPIDS
FLUORESCENCE
ARACHNID
HEXAMER
topic HEMOCYANIN
MALDI-TOF
LIPIDS
FLUORESCENCE
ARACHNID
HEXAMER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus.
Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Fil: Garcia, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Fil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
description VHDL fraction contains hemocyanin as its major apoprotein and transports most of the circulating lipids in the spider Polybetes pythagoricus (Sparassidae). This work shows that subfraction II (the major VHDL component) is composed of a single protein of 420 kDa under native conditions and three subunits, 67, 105 and 121 kDa under denaturing conditions. Circular dichroism indicated that this subfraction contains 20% α-helix, 29% β-sheet, 22.7% turns and 29.7% unordered structures. Comparison of trypsin susceptibility showed that the 105 and 121 kDa subunits were more susceptible indicating that these proteins would be more exposed to the aqueous medium. Peptide mass fingerprinting of the 67 and 105 kDa subunits indicated the 67 kDa subunit is similar to subunit 3 of the spider Cupiennius salei hemocyanin (21% sequence similarity), whereas the 105 kDa subunit is similar to a protein from the mosquito Anopheles gambiae (20% sequence similarity). The N-terminal amino acid sequence from subunit of 121 kDa was also determined. In relation to fatty acids, 16:0, 18:0, 18:1 and 18:2 were found to be the major components. These data provide a better understanding of VHDL subfraction II structure, which is responsible for most lipid transport in the spider P. pythagoricus.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9064
Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana; Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus; Inst Histol Embriol-conicet; Biocell; 39; 1; 6-2015; 33-40
0327-9545
1667-5746
url http://hdl.handle.net/11336/9064
identifier_str_mv Laino, Aldana; Garcia, Fernando; Cunningham, Monica Liliana; Protein characterization and fatty acid composition of VHDL subfraction II of the spider Polybetes pythagoricus; Inst Histol Embriol-conicet; Biocell; 39; 1; 6-2015; 33-40
0327-9545
1667-5746
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.mendoza-conicet.gov.ar/biocell/vol/pdf/39_1/Biocell_MS4038_Cunningham.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inst Histol Embriol-conicet
publisher.none.fl_str_mv Inst Histol Embriol-conicet
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268790488825856
score 12.885934

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