Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes

Autores
Maldonado, Marcos Javier; Albarracín, Virginia Helena; Lara, José Augusto; Ferrero, Marcela Alejandra; Farias, Maria Eugenia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The hypersaline and extreme environments of the so-called high-altitude Andean lakes in the dry central region of the Andes are considered pristine ecosystems of biotechnological interest. By using culture-dependent and -independent methods, we aimed to describe the phylogenetic affiliation and ecological importance of Archaea thriving in extreme, hypersaline lakes and salt flats in the Argentinean and Chilean Puna. For this purpose, water and sediment samples were collected from 14 lakes and salt flats. Denaturing gradient gel electrophoresis (DGGE) indicated that representatives of the phylum Euryarchaeota dominated the microbial community in all samples. Most of the DGGE bands were assigned to the family Halobacteriaceae, while the rest corresponded to Methanocaldococcaceae, Methanobacteriaceae, Methanococcaceae, Methanosarcinaceae, and Methanothermaceae. In addition, isolation procedures yielded single colonies of 53 pure isolates belonging to the following genera: Haloarcula, Halomicrobium, Halopiger, Halorubrum, Natrialba, Natrinema, Natronorubrum, and Natronococcus. All strains proved to be polyextremophiles, with high tolerance to NaCl, UV-B radiation, and high arsenic concentration.
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lara, José Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Archaea
Extremophiles
Halophiles
Arsenicv
Halophiles
Arsenicv
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86145
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakesMaldonado, Marcos JavierAlbarracín, Virginia HelenaLara, José AugustoFerrero, Marcela AlejandraFarias, Maria EugeniaArchaeaExtremophilesHalophilesArsenicvHalophilesArsenicvhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The hypersaline and extreme environments of the so-called high-altitude Andean lakes in the dry central region of the Andes are considered pristine ecosystems of biotechnological interest. By using culture-dependent and -independent methods, we aimed to describe the phylogenetic affiliation and ecological importance of Archaea thriving in extreme, hypersaline lakes and salt flats in the Argentinean and Chilean Puna. For this purpose, water and sediment samples were collected from 14 lakes and salt flats. Denaturing gradient gel electrophoresis (DGGE) indicated that representatives of the phylum Euryarchaeota dominated the microbial community in all samples. Most of the DGGE bands were assigned to the family Halobacteriaceae, while the rest corresponded to Methanocaldococcaceae, Methanobacteriaceae, Methanococcaceae, Methanosarcinaceae, and Methanothermaceae. In addition, isolation procedures yielded single colonies of 53 pure isolates belonging to the following genera: Haloarcula, Halomicrobium, Halopiger, Halorubrum, Natrialba, Natrinema, Natronorubrum, and Natronococcus. All strains proved to be polyextremophiles, with high tolerance to NaCl, UV-B radiation, and high arsenic concentration.Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Lara, José Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaInter-Research2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86145Maldonado, Marcos Javier; Albarracín, Virginia Helena; Lara, José Augusto; Ferrero, Marcela Alejandra; Farias, Maria Eugenia; Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes ; Inter-Research; Aquatic Microbial Ecology; 81; 2; 4-2018; 171-1880948-3055CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.int-res.com/abstracts/ame/v81/n2/p171-188/info:eu-repo/semantics/altIdentifier/doi/10.3354/ame01863info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:26Zoai:ri.conicet.gov.ar:11336/86145instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:26.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
title Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
spellingShingle Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
Maldonado, Marcos Javier
Archaea
Extremophiles
Halophiles
Arsenicv
Halophiles
Arsenicv
title_short Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
title_full Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
title_fullStr Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
title_full_unstemmed Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
title_sort Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes
dc.creator.none.fl_str_mv Maldonado, Marcos Javier
Albarracín, Virginia Helena
Lara, José Augusto
Ferrero, Marcela Alejandra
Farias, Maria Eugenia
author Maldonado, Marcos Javier
author_facet Maldonado, Marcos Javier
Albarracín, Virginia Helena
Lara, José Augusto
Ferrero, Marcela Alejandra
Farias, Maria Eugenia
author_role author
author2 Albarracín, Virginia Helena
Lara, José Augusto
Ferrero, Marcela Alejandra
Farias, Maria Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Archaea
Extremophiles
Halophiles
Arsenicv
Halophiles
Arsenicv
topic Archaea
Extremophiles
Halophiles
Arsenicv
Halophiles
Arsenicv
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The hypersaline and extreme environments of the so-called high-altitude Andean lakes in the dry central region of the Andes are considered pristine ecosystems of biotechnological interest. By using culture-dependent and -independent methods, we aimed to describe the phylogenetic affiliation and ecological importance of Archaea thriving in extreme, hypersaline lakes and salt flats in the Argentinean and Chilean Puna. For this purpose, water and sediment samples were collected from 14 lakes and salt flats. Denaturing gradient gel electrophoresis (DGGE) indicated that representatives of the phylum Euryarchaeota dominated the microbial community in all samples. Most of the DGGE bands were assigned to the family Halobacteriaceae, while the rest corresponded to Methanocaldococcaceae, Methanobacteriaceae, Methanococcaceae, Methanosarcinaceae, and Methanothermaceae. In addition, isolation procedures yielded single colonies of 53 pure isolates belonging to the following genera: Haloarcula, Halomicrobium, Halopiger, Halorubrum, Natrialba, Natrinema, Natronorubrum, and Natronococcus. All strains proved to be polyextremophiles, with high tolerance to NaCl, UV-B radiation, and high arsenic concentration.
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lara, José Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description The hypersaline and extreme environments of the so-called high-altitude Andean lakes in the dry central region of the Andes are considered pristine ecosystems of biotechnological interest. By using culture-dependent and -independent methods, we aimed to describe the phylogenetic affiliation and ecological importance of Archaea thriving in extreme, hypersaline lakes and salt flats in the Argentinean and Chilean Puna. For this purpose, water and sediment samples were collected from 14 lakes and salt flats. Denaturing gradient gel electrophoresis (DGGE) indicated that representatives of the phylum Euryarchaeota dominated the microbial community in all samples. Most of the DGGE bands were assigned to the family Halobacteriaceae, while the rest corresponded to Methanocaldococcaceae, Methanobacteriaceae, Methanococcaceae, Methanosarcinaceae, and Methanothermaceae. In addition, isolation procedures yielded single colonies of 53 pure isolates belonging to the following genera: Haloarcula, Halomicrobium, Halopiger, Halorubrum, Natrialba, Natrinema, Natronorubrum, and Natronococcus. All strains proved to be polyextremophiles, with high tolerance to NaCl, UV-B radiation, and high arsenic concentration.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86145
Maldonado, Marcos Javier; Albarracín, Virginia Helena; Lara, José Augusto; Ferrero, Marcela Alejandra; Farias, Maria Eugenia; Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes ; Inter-Research; Aquatic Microbial Ecology; 81; 2; 4-2018; 171-188
0948-3055
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86145
identifier_str_mv Maldonado, Marcos Javier; Albarracín, Virginia Helena; Lara, José Augusto; Ferrero, Marcela Alejandra; Farias, Maria Eugenia; Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes ; Inter-Research; Aquatic Microbial Ecology; 81; 2; 4-2018; 171-188
0948-3055
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.int-res.com/abstracts/ame/v81/n2/p171-188/
info:eu-repo/semantics/altIdentifier/doi/10.3354/ame01863
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inter-Research
publisher.none.fl_str_mv Inter-Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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