Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications

Autores
Benavidez, Tomás Enrique; Baruzzi, Ana Maria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The analytical response of amperometric glucose electrodes was explained in the framework of the properties of hydrogels with different muc/chit ratio at pH = 7.00 used as immobilization matrix for glucose oxidase. The continuous increase in the sensitivity of the electrodes with the amount of chitosan was attributed to a more efficient crosslinking without affecting the enzyme activity. The results were compared with those obtained using the same matrices to immobilize Oxalate Oxidase at pH = 2.85 where it has the highest activity. A maximum sensitivity at muc/chit 70/30 ratio for these oxalate electrodes was observed. Decrease in permeability of negatively charged oxalate with the chitosan content in the matrices, in spite of the high partition coefficient, explains the analytical differences with glucose electrodes. Swelling and viscoelasticity of the hydrogels allowed to asses the importance of a hydrophilic environment for the enzyme in both cases. © 2011 Elsevier Ltd. All rights reserved.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Biosensor
Enzymes
Hydrogel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61792

id CONICETDig_655f24ee90d7426abdc9e32301febea3
oai_identifier_str oai:ri.conicet.gov.ar:11336/61792
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applicationsBenavidez, Tomás EnriqueBaruzzi, Ana MariaBiosensorEnzymesHydrogelhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The analytical response of amperometric glucose electrodes was explained in the framework of the properties of hydrogels with different muc/chit ratio at pH = 7.00 used as immobilization matrix for glucose oxidase. The continuous increase in the sensitivity of the electrodes with the amount of chitosan was attributed to a more efficient crosslinking without affecting the enzyme activity. The results were compared with those obtained using the same matrices to immobilize Oxalate Oxidase at pH = 2.85 where it has the highest activity. A maximum sensitivity at muc/chit 70/30 ratio for these oxalate electrodes was observed. Decrease in permeability of negatively charged oxalate with the chitosan content in the matrices, in spite of the high partition coefficient, explains the analytical differences with glucose electrodes. Swelling and viscoelasticity of the hydrogels allowed to asses the importance of a hydrophilic environment for the enzyme in both cases. © 2011 Elsevier Ltd. All rights reserved.Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61792Benavidez, Tomás Enrique; Baruzzi, Ana Maria; Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications; Elsevier; Polymer; 53; 2; 1-2012; 438-4440032-3861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/https://www.sciencedirect.com/science/article/pii/S0032386111010202info:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymer.2011.12.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:22Zoai:ri.conicet.gov.ar:11336/61792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:22.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
title Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
spellingShingle Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
Benavidez, Tomás Enrique
Biosensor
Enzymes
Hydrogel
title_short Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
title_full Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
title_fullStr Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
title_full_unstemmed Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
title_sort Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
dc.creator.none.fl_str_mv Benavidez, Tomás Enrique
Baruzzi, Ana Maria
author Benavidez, Tomás Enrique
author_facet Benavidez, Tomás Enrique
Baruzzi, Ana Maria
author_role author
author2 Baruzzi, Ana Maria
author2_role author
dc.subject.none.fl_str_mv Biosensor
Enzymes
Hydrogel
topic Biosensor
Enzymes
Hydrogel
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The analytical response of amperometric glucose electrodes was explained in the framework of the properties of hydrogels with different muc/chit ratio at pH = 7.00 used as immobilization matrix for glucose oxidase. The continuous increase in the sensitivity of the electrodes with the amount of chitosan was attributed to a more efficient crosslinking without affecting the enzyme activity. The results were compared with those obtained using the same matrices to immobilize Oxalate Oxidase at pH = 2.85 where it has the highest activity. A maximum sensitivity at muc/chit 70/30 ratio for these oxalate electrodes was observed. Decrease in permeability of negatively charged oxalate with the chitosan content in the matrices, in spite of the high partition coefficient, explains the analytical differences with glucose electrodes. Swelling and viscoelasticity of the hydrogels allowed to asses the importance of a hydrophilic environment for the enzyme in both cases. © 2011 Elsevier Ltd. All rights reserved.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description The analytical response of amperometric glucose electrodes was explained in the framework of the properties of hydrogels with different muc/chit ratio at pH = 7.00 used as immobilization matrix for glucose oxidase. The continuous increase in the sensitivity of the electrodes with the amount of chitosan was attributed to a more efficient crosslinking without affecting the enzyme activity. The results were compared with those obtained using the same matrices to immobilize Oxalate Oxidase at pH = 2.85 where it has the highest activity. A maximum sensitivity at muc/chit 70/30 ratio for these oxalate electrodes was observed. Decrease in permeability of negatively charged oxalate with the chitosan content in the matrices, in spite of the high partition coefficient, explains the analytical differences with glucose electrodes. Swelling and viscoelasticity of the hydrogels allowed to asses the importance of a hydrophilic environment for the enzyme in both cases. © 2011 Elsevier Ltd. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61792
Benavidez, Tomás Enrique; Baruzzi, Ana Maria; Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications; Elsevier; Polymer; 53; 2; 1-2012; 438-444
0032-3861
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61792
identifier_str_mv Benavidez, Tomás Enrique; Baruzzi, Ana Maria; Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications; Elsevier; Polymer; 53; 2; 1-2012; 438-444
0032-3861
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/https://www.sciencedirect.com/science/article/pii/S0032386111010202
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymer.2011.12.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613827234103296
score 13.070432