Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix

Autores
Benavidez, Tomás Enrique; Capra, Raul H.; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An amperometric electrode for oxalate determination immobilizing the oxalate oxidase in a mucin/chitosan (muc/chit) gel with glutaraldehyde as crosslinking agent is presented. The effect of muc/chit weight ratio and volume percent (vol.%) of glutaraldehyde was studied. A very low dynamic response was observed in the case of 100% chitosan with 5 vol.% crosslinking agent. The addition of mucin to chitosan for enzyme immobilization resulted in a biosensor with much better performance, concerning to dynamic response, sensitivity, and stability, with 75% of the initial response after two months. The ratio muc/chit 70/30 was considered optimum for the immobilization. A slight crosslinking and the incorporation of mucin largely influences the swelling and diffusion of the analyte; a direct effect of these properties on the calibration slope was found; the hydrophilic environment for the biomolecule also favor the enzymatic activity through a higher enzyme-substrate interaction.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Capra, Raul H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Biosensor
Chitosan
Electrochemistry
Hidrogel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26284
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrixBenavidez, Tomás EnriqueCapra, Raul H.Alvarez Igarzabal, Cecilia InesBaruzzi, Ana MariaBiosensorChitosanElectrochemistryHidrogelhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An amperometric electrode for oxalate determination immobilizing the oxalate oxidase in a mucin/chitosan (muc/chit) gel with glutaraldehyde as crosslinking agent is presented. The effect of muc/chit weight ratio and volume percent (vol.%) of glutaraldehyde was studied. A very low dynamic response was observed in the case of 100% chitosan with 5 vol.% crosslinking agent. The addition of mucin to chitosan for enzyme immobilization resulted in a biosensor with much better performance, concerning to dynamic response, sensitivity, and stability, with 75% of the initial response after two months. The ratio muc/chit 70/30 was considered optimum for the immobilization. A slight crosslinking and the incorporation of mucin largely influences the swelling and diffusion of the analyte; a direct effect of these properties on the calibration slope was found; the hydrophilic environment for the biomolecule also favor the enzymatic activity through a higher enzyme-substrate interaction.Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Capra, Raul H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26284Benavidez, Tomás Enrique; Capra, Raul H.; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix; Wiley VCH Verlag; Electroanalysis; 21; 7; 4-2009; 837-8431040-03971521-4109CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.200804482/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200804482info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:37Zoai:ri.conicet.gov.ar:11336/26284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:37.661CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
title Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
spellingShingle Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
Benavidez, Tomás Enrique
Biosensor
Chitosan
Electrochemistry
Hidrogel
title_short Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
title_full Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
title_fullStr Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
title_full_unstemmed Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
title_sort Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix
dc.creator.none.fl_str_mv Benavidez, Tomás Enrique
Capra, Raul H.
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
author Benavidez, Tomás Enrique
author_facet Benavidez, Tomás Enrique
Capra, Raul H.
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
author_role author
author2 Capra, Raul H.
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv Biosensor
Chitosan
Electrochemistry
Hidrogel
topic Biosensor
Chitosan
Electrochemistry
Hidrogel
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An amperometric electrode for oxalate determination immobilizing the oxalate oxidase in a mucin/chitosan (muc/chit) gel with glutaraldehyde as crosslinking agent is presented. The effect of muc/chit weight ratio and volume percent (vol.%) of glutaraldehyde was studied. A very low dynamic response was observed in the case of 100% chitosan with 5 vol.% crosslinking agent. The addition of mucin to chitosan for enzyme immobilization resulted in a biosensor with much better performance, concerning to dynamic response, sensitivity, and stability, with 75% of the initial response after two months. The ratio muc/chit 70/30 was considered optimum for the immobilization. A slight crosslinking and the incorporation of mucin largely influences the swelling and diffusion of the analyte; a direct effect of these properties on the calibration slope was found; the hydrophilic environment for the biomolecule also favor the enzymatic activity through a higher enzyme-substrate interaction.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Capra, Raul H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description An amperometric electrode for oxalate determination immobilizing the oxalate oxidase in a mucin/chitosan (muc/chit) gel with glutaraldehyde as crosslinking agent is presented. The effect of muc/chit weight ratio and volume percent (vol.%) of glutaraldehyde was studied. A very low dynamic response was observed in the case of 100% chitosan with 5 vol.% crosslinking agent. The addition of mucin to chitosan for enzyme immobilization resulted in a biosensor with much better performance, concerning to dynamic response, sensitivity, and stability, with 75% of the initial response after two months. The ratio muc/chit 70/30 was considered optimum for the immobilization. A slight crosslinking and the incorporation of mucin largely influences the swelling and diffusion of the analyte; a direct effect of these properties on the calibration slope was found; the hydrophilic environment for the biomolecule also favor the enzymatic activity through a higher enzyme-substrate interaction.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26284
Benavidez, Tomás Enrique; Capra, Raul H.; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix; Wiley VCH Verlag; Electroanalysis; 21; 7; 4-2009; 837-843
1040-0397
1521-4109
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26284
identifier_str_mv Benavidez, Tomás Enrique; Capra, Raul H.; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Amperometric biosensor based on immobilization of oxalate oxidase in a mucin/chitosan matrix; Wiley VCH Verlag; Electroanalysis; 21; 7; 4-2009; 837-843
1040-0397
1521-4109
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.200804482/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200804482
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083426254323712
score 13.22299

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