Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor

Autores
Benavidez, Tomás Enrique; Alvarez, Cecilia Ines; Baruzzi, Ana Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Immobilization conditions of biomolecules play an important role in a biosensor performance. In this paper the response of amperometric electrodes for oxalate is related with some physicochemical properties of the matrices where oxalate oxidase was immobilized. Swelling indexes, partition coefficients and permeability of oxalate in mucin/chitosan matrices in a pH = 2.85 succinic acid solution were measured. These properties were also determined in the 70/30 mucin/chitosan matrix in solutions containing succinic acid and sulfate or chloride anions at the same pH value. The results indicate that the sensitivity of the biosensors is mainly determined by the swelling index of the matrix. High slopes of the calibration curves of the electrodes were obtained with highly swollen matrices indicating the importance of a hydrophilic environment for a good enzymatic response.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Materia
Polymer
Mucin
Chitosan
Biosensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14990
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensorBenavidez, Tomás EnriqueAlvarez, Cecilia InesBaruzzi, Ana MariaPolymerMucinChitosanBiosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Immobilization conditions of biomolecules play an important role in a biosensor performance. In this paper the response of amperometric electrodes for oxalate is related with some physicochemical properties of the matrices where oxalate oxidase was immobilized. Swelling indexes, partition coefficients and permeability of oxalate in mucin/chitosan matrices in a pH = 2.85 succinic acid solution were measured. These properties were also determined in the 70/30 mucin/chitosan matrix in solutions containing succinic acid and sulfate or chloride anions at the same pH value. The results indicate that the sensitivity of the biosensors is mainly determined by the swelling index of the matrix. High slopes of the calibration curves of the electrodes were obtained with highly swollen matrices indicating the importance of a hydrophilic environment for a good enzymatic response.Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; ArgentinaElsevier2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14990Benavidez, Tomás Enrique; Alvarez, Cecilia Ines; Baruzzi, Ana Maria; Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor; Elsevier; Sensors And Actuators B: Chemical; 143; 2; 9-2010; 660-6650925-4005enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400509007990info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2009.10.031info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:54Zoai:ri.conicet.gov.ar:11336/14990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:54.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
title Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
spellingShingle Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
Benavidez, Tomás Enrique
Polymer
Mucin
Chitosan
Biosensor
title_short Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
title_full Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
title_fullStr Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
title_full_unstemmed Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
title_sort Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor
dc.creator.none.fl_str_mv Benavidez, Tomás Enrique
Alvarez, Cecilia Ines
Baruzzi, Ana Maria
author Benavidez, Tomás Enrique
author_facet Benavidez, Tomás Enrique
Alvarez, Cecilia Ines
Baruzzi, Ana Maria
author_role author
author2 Alvarez, Cecilia Ines
Baruzzi, Ana Maria
author2_role author
author
dc.subject.none.fl_str_mv Polymer
Mucin
Chitosan
Biosensor
topic Polymer
Mucin
Chitosan
Biosensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Immobilization conditions of biomolecules play an important role in a biosensor performance. In this paper the response of amperometric electrodes for oxalate is related with some physicochemical properties of the matrices where oxalate oxidase was immobilized. Swelling indexes, partition coefficients and permeability of oxalate in mucin/chitosan matrices in a pH = 2.85 succinic acid solution were measured. These properties were also determined in the 70/30 mucin/chitosan matrix in solutions containing succinic acid and sulfate or chloride anions at the same pH value. The results indicate that the sensitivity of the biosensors is mainly determined by the swelling index of the matrix. High slopes of the calibration curves of the electrodes were obtained with highly swollen matrices indicating the importance of a hydrophilic environment for a good enzymatic response.
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones en Físicoquímica de Córdoba; Argentina; Argentina. Universidad Nacional de Córdoba; Argentina
description Immobilization conditions of biomolecules play an important role in a biosensor performance. In this paper the response of amperometric electrodes for oxalate is related with some physicochemical properties of the matrices where oxalate oxidase was immobilized. Swelling indexes, partition coefficients and permeability of oxalate in mucin/chitosan matrices in a pH = 2.85 succinic acid solution were measured. These properties were also determined in the 70/30 mucin/chitosan matrix in solutions containing succinic acid and sulfate or chloride anions at the same pH value. The results indicate that the sensitivity of the biosensors is mainly determined by the swelling index of the matrix. High slopes of the calibration curves of the electrodes were obtained with highly swollen matrices indicating the importance of a hydrophilic environment for a good enzymatic response.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14990
Benavidez, Tomás Enrique; Alvarez, Cecilia Ines; Baruzzi, Ana Maria; Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor; Elsevier; Sensors And Actuators B: Chemical; 143; 2; 9-2010; 660-665
0925-4005
url http://hdl.handle.net/11336/14990
identifier_str_mv Benavidez, Tomás Enrique; Alvarez, Cecilia Ines; Baruzzi, Ana Maria; Physicochemical properties of a mucin/chitosan matrix used for the development of an oxalate biosensor; Elsevier; Sensors And Actuators B: Chemical; 143; 2; 9-2010; 660-665
0925-4005
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400509007990
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2009.10.031
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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