Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress

Autores
Zilli, Carla Giannina; Santa Cruz, Diego Mario; Balestrasse, Karina Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The exogenous application of carbon monoxide (CO) is a valuable strategy which enables study of the effects under different stress conditions. However, in this experimental model a true endogenous CO production by plants cannot be measured. In this work, so as to achieve an elevated sensitivity and to avoid invasive techniques, we quantify the endogenous CO production by tissues in salt-treated soybean plants through gas chromatography coupled to a reduction gas detector. This technique allows short and room temperature incubation of intact tissues and homogenates. We found that a 200. mM NaCl treatment induces total CO production in leaves and roots. The sensitivity of the technique offers no correlation between this increment and heme oxygenase (HO) activity measured as a function of CO production. We also found that untreated soybean plants continue to produce significant CO levels up to 7 days post planting, after which CO content decreases to a third and remains constant in the next days. However, HO activity does not change throughout these days. The data here reported shows that HO activity is not the main source of CO in soybean plants. We discuss alternative sources that could be implicated in this production. Taking our own results and data reported by other colleagues, we propose lipid peroxidation and ureide metabolism as potential sources of CO.
Fil: Zilli, Carla Giannina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. University of Stanford; Estados Unidos
Fil: Santa Cruz, Diego Mario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biologia Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
Hemeoxygenase
Carbon Monoxide
Soybean
Salt Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3106
Acceder
id CONICETDig_641da0733269287dba8acf8148407884
oai_identifier_str oai:ri.conicet.gov.ar:11336/3106
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stressZilli, Carla GianninaSanta Cruz, Diego MarioBalestrasse, Karina BeatrizHemeoxygenaseCarbon MonoxideSoybeanSalt Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The exogenous application of carbon monoxide (CO) is a valuable strategy which enables study of the effects under different stress conditions. However, in this experimental model a true endogenous CO production by plants cannot be measured. In this work, so as to achieve an elevated sensitivity and to avoid invasive techniques, we quantify the endogenous CO production by tissues in salt-treated soybean plants through gas chromatography coupled to a reduction gas detector. This technique allows short and room temperature incubation of intact tissues and homogenates. We found that a 200. mM NaCl treatment induces total CO production in leaves and roots. The sensitivity of the technique offers no correlation between this increment and heme oxygenase (HO) activity measured as a function of CO production. We also found that untreated soybean plants continue to produce significant CO levels up to 7 days post planting, after which CO content decreases to a third and remains constant in the next days. However, HO activity does not change throughout these days. The data here reported shows that HO activity is not the main source of CO in soybean plants. We discuss alternative sources that could be implicated in this production. Taking our own results and data reported by other colleagues, we propose lipid peroxidation and ureide metabolism as potential sources of CO.Fil: Zilli, Carla Giannina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. University of Stanford; Estados UnidosFil: Santa Cruz, Diego Mario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biologia Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaElsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3106Zilli, Carla Giannina; Santa Cruz, Diego Mario; Balestrasse, Karina Beatriz; Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress; Elsevier; Environmental and Experimental Botany; 102; 16; 12-2013; 1-60098-8472enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0098847214000185info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2014.01.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:42:42Zoai:ri.conicet.gov.ar:11336/3106instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:42:42.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
title Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
spellingShingle Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
Zilli, Carla Giannina
Hemeoxygenase
Carbon Monoxide
Soybean
Salt Stress
title_short Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
title_full Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
title_fullStr Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
title_full_unstemmed Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
title_sort Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress
dc.creator.none.fl_str_mv Zilli, Carla Giannina
Santa Cruz, Diego Mario
Balestrasse, Karina Beatriz
author Zilli, Carla Giannina
author_facet Zilli, Carla Giannina
Santa Cruz, Diego Mario
Balestrasse, Karina Beatriz
author_role author
author2 Santa Cruz, Diego Mario
Balestrasse, Karina Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Hemeoxygenase
Carbon Monoxide
Soybean
Salt Stress
topic Hemeoxygenase
Carbon Monoxide
Soybean
Salt Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The exogenous application of carbon monoxide (CO) is a valuable strategy which enables study of the effects under different stress conditions. However, in this experimental model a true endogenous CO production by plants cannot be measured. In this work, so as to achieve an elevated sensitivity and to avoid invasive techniques, we quantify the endogenous CO production by tissues in salt-treated soybean plants through gas chromatography coupled to a reduction gas detector. This technique allows short and room temperature incubation of intact tissues and homogenates. We found that a 200. mM NaCl treatment induces total CO production in leaves and roots. The sensitivity of the technique offers no correlation between this increment and heme oxygenase (HO) activity measured as a function of CO production. We also found that untreated soybean plants continue to produce significant CO levels up to 7 days post planting, after which CO content decreases to a third and remains constant in the next days. However, HO activity does not change throughout these days. The data here reported shows that HO activity is not the main source of CO in soybean plants. We discuss alternative sources that could be implicated in this production. Taking our own results and data reported by other colleagues, we propose lipid peroxidation and ureide metabolism as potential sources of CO.
Fil: Zilli, Carla Giannina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. University of Stanford; Estados Unidos
Fil: Santa Cruz, Diego Mario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biologia Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description The exogenous application of carbon monoxide (CO) is a valuable strategy which enables study of the effects under different stress conditions. However, in this experimental model a true endogenous CO production by plants cannot be measured. In this work, so as to achieve an elevated sensitivity and to avoid invasive techniques, we quantify the endogenous CO production by tissues in salt-treated soybean plants through gas chromatography coupled to a reduction gas detector. This technique allows short and room temperature incubation of intact tissues and homogenates. We found that a 200. mM NaCl treatment induces total CO production in leaves and roots. The sensitivity of the technique offers no correlation between this increment and heme oxygenase (HO) activity measured as a function of CO production. We also found that untreated soybean plants continue to produce significant CO levels up to 7 days post planting, after which CO content decreases to a third and remains constant in the next days. However, HO activity does not change throughout these days. The data here reported shows that HO activity is not the main source of CO in soybean plants. We discuss alternative sources that could be implicated in this production. Taking our own results and data reported by other colleagues, we propose lipid peroxidation and ureide metabolism as potential sources of CO.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3106
Zilli, Carla Giannina; Santa Cruz, Diego Mario; Balestrasse, Karina Beatriz; Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress; Elsevier; Environmental and Experimental Botany; 102; 16; 12-2013; 1-6
0098-8472
url http://hdl.handle.net/11336/3106
identifier_str_mv Zilli, Carla Giannina; Santa Cruz, Diego Mario; Balestrasse, Karina Beatriz; Heme oxygenase-independent endogenous production of carbon monoxide by soybean plants subjected to salt stress; Elsevier; Environmental and Experimental Botany; 102; 16; 12-2013; 1-6
0098-8472
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0098847214000185
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2014.01.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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