Heme oxygenase activity and oxidative stress signalling in soybean leaves

Autores
Balestrasse, Karina Beatriz; Noriega, Guillermo Osvaldo; Batlle, Alcira María del C.; Tomaro, Maria Lujan
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against oxidative stress. Here, we have investigated for the first time the possible signal transduction pathways involved in heme oxygenase-1 induction in leaves of soybean plants. Treatment with 200 μM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased 70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin (protein phosphatases inhibitor), LaCl 3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin- 1-one (ODQ, guanylate cyclase inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2-, which were again prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress, triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship between oxidative stress generation and heme oxygenase induction in higher plants.
Fil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Noriega, Guillermo Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CD TOXICITY
HEME OXYGENASE
LEAVES
SIGNALING PATHWAYS
SOYBEAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125459
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/125459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heme oxygenase activity and oxidative stress signalling in soybean leavesBalestrasse, Karina BeatrizNoriega, Guillermo OsvaldoBatlle, Alcira María del C.Tomaro, Maria LujanCD TOXICITYHEME OXYGENASELEAVESSIGNALING PATHWAYSSOYBEANhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against oxidative stress. Here, we have investigated for the first time the possible signal transduction pathways involved in heme oxygenase-1 induction in leaves of soybean plants. Treatment with 200 μM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased 70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin (protein phosphatases inhibitor), LaCl 3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin- 1-one (ODQ, guanylate cyclase inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2-, which were again prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress, triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship between oxidative stress generation and heme oxygenase induction in higher plants.Fil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Noriega, Guillermo Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125459Balestrasse, Karina Beatriz; Noriega, Guillermo Osvaldo; Batlle, Alcira María del C.; Tomaro, Maria Lujan; Heme oxygenase activity and oxidative stress signalling in soybean leaves; Elsevier Ireland; Plant Science; 170; 2; 2-2006; 339-3460168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168945205003316info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.09.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:21Zoai:ri.conicet.gov.ar:11336/125459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:21.92CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heme oxygenase activity and oxidative stress signalling in soybean leaves
title Heme oxygenase activity and oxidative stress signalling in soybean leaves
spellingShingle Heme oxygenase activity and oxidative stress signalling in soybean leaves
Balestrasse, Karina Beatriz
CD TOXICITY
HEME OXYGENASE
LEAVES
SIGNALING PATHWAYS
SOYBEAN
title_short Heme oxygenase activity and oxidative stress signalling in soybean leaves
title_full Heme oxygenase activity and oxidative stress signalling in soybean leaves
title_fullStr Heme oxygenase activity and oxidative stress signalling in soybean leaves
title_full_unstemmed Heme oxygenase activity and oxidative stress signalling in soybean leaves
title_sort Heme oxygenase activity and oxidative stress signalling in soybean leaves
dc.creator.none.fl_str_mv Balestrasse, Karina Beatriz
Noriega, Guillermo Osvaldo
Batlle, Alcira María del C.
Tomaro, Maria Lujan
author Balestrasse, Karina Beatriz
author_facet Balestrasse, Karina Beatriz
Noriega, Guillermo Osvaldo
Batlle, Alcira María del C.
Tomaro, Maria Lujan
author_role author
author2 Noriega, Guillermo Osvaldo
Batlle, Alcira María del C.
Tomaro, Maria Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv CD TOXICITY
HEME OXYGENASE
LEAVES
SIGNALING PATHWAYS
SOYBEAN
topic CD TOXICITY
HEME OXYGENASE
LEAVES
SIGNALING PATHWAYS
SOYBEAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against oxidative stress. Here, we have investigated for the first time the possible signal transduction pathways involved in heme oxygenase-1 induction in leaves of soybean plants. Treatment with 200 μM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased 70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin (protein phosphatases inhibitor), LaCl 3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin- 1-one (ODQ, guanylate cyclase inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2-, which were again prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress, triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship between oxidative stress generation and heme oxygenase induction in higher plants.
Fil: Balestrasse, Karina Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Noriega, Guillermo Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against oxidative stress. Here, we have investigated for the first time the possible signal transduction pathways involved in heme oxygenase-1 induction in leaves of soybean plants. Treatment with 200 μM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased 70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin (protein phosphatases inhibitor), LaCl 3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin- 1-one (ODQ, guanylate cyclase inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2-, which were again prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress, triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship between oxidative stress generation and heme oxygenase induction in higher plants.
publishDate 2006
dc.date.none.fl_str_mv 2006-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125459
Balestrasse, Karina Beatriz; Noriega, Guillermo Osvaldo; Batlle, Alcira María del C.; Tomaro, Maria Lujan; Heme oxygenase activity and oxidative stress signalling in soybean leaves; Elsevier Ireland; Plant Science; 170; 2; 2-2006; 339-346
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125459
identifier_str_mv Balestrasse, Karina Beatriz; Noriega, Guillermo Osvaldo; Batlle, Alcira María del C.; Tomaro, Maria Lujan; Heme oxygenase activity and oxidative stress signalling in soybean leaves; Elsevier Ireland; Plant Science; 170; 2; 2-2006; 339-346
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168945205003316
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.09.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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