Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging

Autores
Salas, Sabrina Rosicler; Pascual, Ana Clara; Milano, Pablo Gustavo; Pasquaré, Susana Juana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The availability of the main neuroprotective endocannabinoid, 2-ara- chidonoylglycerol (2-AG), was found to decrease in rat cerebral cor- tex (CC) synaptosomes (syn) during aging. It was also demonstrat- ed that 2-AG metabolism can be modulated by its own receptors. Moreover, phytocannabinoids (PC) present in Cannabis extracts (CE), have been proposed as neuroprotective agents and are be- ing used in several studies to treat neurodegenerative processes. The aim of this study was to evaluate if THC present in CE could modulate 2-AG hydrolysis by monoacylglycerol lipase (MAGL). To this end, delta-9-tetrahydrolcannabinol (THC) enriched CE were ob- tained from Cannabis Y Griega female flowers, previously grinded and heated 115°C for 40 min. Extractions were performed by add- ing 5% (w/v) ethanol, vortexed, sonicated and agitated. The mixture was centrifuged and the supernatant was evaporated under stream of N2 . THC, cannabidiol (CBD) and cannabinol (CBN) were quan- tified by HPLC. CC syn from adult (4-6 months) and aged (24-26 months) rats were isolated by differential centrifugation and purified in ficoll gradients. MAGL activity was assessed by incubating syn with either THC enriched CE (10-3-50 μM THC) or 1 μM pure THC, and [3 H]-MAG, simultaneously. It was observed that CE containing 1 μM THC decreased MAGL activity in aged (p=0.0166) but not in adult syn (p>0.05). However, 1 μM pure THC failed to modulate 2-AG hydrolysis (p>0.05). This findings, could suggest that THC enriched CE could attenuate the deregulation of 2-AG metabolism observed in aging, and that this effect could only be generated when syn are exposed to the PC in the presence of the entire CE.
Fil: Salas, Sabrina Rosicler. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De Nanomedicinas
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
2-ARACHIDONOYLGLYCEROL
CANNABIS EXTRACTS
AGING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184971
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during agingSalas, Sabrina RosiclerPascual, Ana ClaraMilano, Pablo GustavoPasquaré, Susana Juana2-ARACHIDONOYLGLYCEROLCANNABIS EXTRACTSAGINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The availability of the main neuroprotective endocannabinoid, 2-ara- chidonoylglycerol (2-AG), was found to decrease in rat cerebral cor- tex (CC) synaptosomes (syn) during aging. It was also demonstrat- ed that 2-AG metabolism can be modulated by its own receptors. Moreover, phytocannabinoids (PC) present in Cannabis extracts (CE), have been proposed as neuroprotective agents and are be- ing used in several studies to treat neurodegenerative processes. The aim of this study was to evaluate if THC present in CE could modulate 2-AG hydrolysis by monoacylglycerol lipase (MAGL). To this end, delta-9-tetrahydrolcannabinol (THC) enriched CE were ob- tained from Cannabis Y Griega female flowers, previously grinded and heated 115°C for 40 min. Extractions were performed by add- ing 5% (w/v) ethanol, vortexed, sonicated and agitated. The mixture was centrifuged and the supernatant was evaporated under stream of N2 . THC, cannabidiol (CBD) and cannabinol (CBN) were quan- tified by HPLC. CC syn from adult (4-6 months) and aged (24-26 months) rats were isolated by differential centrifugation and purified in ficoll gradients. MAGL activity was assessed by incubating syn with either THC enriched CE (10-3-50 μM THC) or 1 μM pure THC, and [3 H]-MAG, simultaneously. It was observed that CE containing 1 μM THC decreased MAGL activity in aged (p=0.0166) but not in adult syn (p>0.05). However, 1 μM pure THC failed to modulate 2-AG hydrolysis (p>0.05). This findings, could suggest that THC enriched CE could attenuate the deregulation of 2-AG metabolism observed in aging, and that this effect could only be generated when syn are exposed to the PC in the presence of the entire CE.Fil: Salas, Sabrina Rosicler. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De NanomedicinasBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184971Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De Nanomedicinas; Buenos Aires; Argentina; 2021; 187-1871669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:44:20Zoai:ri.conicet.gov.ar:11336/184971instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:44:21.083CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
title Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
spellingShingle Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
Salas, Sabrina Rosicler
2-ARACHIDONOYLGLYCEROL
CANNABIS EXTRACTS
AGING
title_short Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
title_full Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
title_fullStr Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
title_full_unstemmed Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
title_sort Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging
dc.creator.none.fl_str_mv Salas, Sabrina Rosicler
Pascual, Ana Clara
Milano, Pablo Gustavo
Pasquaré, Susana Juana
author Salas, Sabrina Rosicler
author_facet Salas, Sabrina Rosicler
Pascual, Ana Clara
Milano, Pablo Gustavo
Pasquaré, Susana Juana
author_role author
author2 Pascual, Ana Clara
Milano, Pablo Gustavo
Pasquaré, Susana Juana
author2_role author
author
author
dc.subject.none.fl_str_mv 2-ARACHIDONOYLGLYCEROL
CANNABIS EXTRACTS
AGING
topic 2-ARACHIDONOYLGLYCEROL
CANNABIS EXTRACTS
AGING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The availability of the main neuroprotective endocannabinoid, 2-ara- chidonoylglycerol (2-AG), was found to decrease in rat cerebral cor- tex (CC) synaptosomes (syn) during aging. It was also demonstrat- ed that 2-AG metabolism can be modulated by its own receptors. Moreover, phytocannabinoids (PC) present in Cannabis extracts (CE), have been proposed as neuroprotective agents and are be- ing used in several studies to treat neurodegenerative processes. The aim of this study was to evaluate if THC present in CE could modulate 2-AG hydrolysis by monoacylglycerol lipase (MAGL). To this end, delta-9-tetrahydrolcannabinol (THC) enriched CE were ob- tained from Cannabis Y Griega female flowers, previously grinded and heated 115°C for 40 min. Extractions were performed by add- ing 5% (w/v) ethanol, vortexed, sonicated and agitated. The mixture was centrifuged and the supernatant was evaporated under stream of N2 . THC, cannabidiol (CBD) and cannabinol (CBN) were quan- tified by HPLC. CC syn from adult (4-6 months) and aged (24-26 months) rats were isolated by differential centrifugation and purified in ficoll gradients. MAGL activity was assessed by incubating syn with either THC enriched CE (10-3-50 μM THC) or 1 μM pure THC, and [3 H]-MAG, simultaneously. It was observed that CE containing 1 μM THC decreased MAGL activity in aged (p=0.0166) but not in adult syn (p>0.05). However, 1 μM pure THC failed to modulate 2-AG hydrolysis (p>0.05). This findings, could suggest that THC enriched CE could attenuate the deregulation of 2-AG metabolism observed in aging, and that this effect could only be generated when syn are exposed to the PC in the presence of the entire CE.
Fil: Salas, Sabrina Rosicler. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De Nanomedicinas
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description The availability of the main neuroprotective endocannabinoid, 2-ara- chidonoylglycerol (2-AG), was found to decrease in rat cerebral cor- tex (CC) synaptosomes (syn) during aging. It was also demonstrat- ed that 2-AG metabolism can be modulated by its own receptors. Moreover, phytocannabinoids (PC) present in Cannabis extracts (CE), have been proposed as neuroprotective agents and are be- ing used in several studies to treat neurodegenerative processes. The aim of this study was to evaluate if THC present in CE could modulate 2-AG hydrolysis by monoacylglycerol lipase (MAGL). To this end, delta-9-tetrahydrolcannabinol (THC) enriched CE were ob- tained from Cannabis Y Griega female flowers, previously grinded and heated 115°C for 40 min. Extractions were performed by add- ing 5% (w/v) ethanol, vortexed, sonicated and agitated. The mixture was centrifuged and the supernatant was evaporated under stream of N2 . THC, cannabidiol (CBD) and cannabinol (CBN) were quan- tified by HPLC. CC syn from adult (4-6 months) and aged (24-26 months) rats were isolated by differential centrifugation and purified in ficoll gradients. MAGL activity was assessed by incubating syn with either THC enriched CE (10-3-50 μM THC) or 1 μM pure THC, and [3 H]-MAG, simultaneously. It was observed that CE containing 1 μM THC decreased MAGL activity in aged (p=0.0166) but not in adult syn (p>0.05). However, 1 μM pure THC failed to modulate 2-AG hydrolysis (p>0.05). This findings, could suggest that THC enriched CE could attenuate the deregulation of 2-AG metabolism observed in aging, and that this effect could only be generated when syn are exposed to the PC in the presence of the entire CE.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184971
Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De Nanomedicinas; Buenos Aires; Argentina; 2021; 187-187
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184971
identifier_str_mv Regulation of synaptosomal 2-arachidonoylglyceol hydrolysis by Cannabis extracts during aging; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual De La Asociación Argentina De Nanomedicinas; Buenos Aires; Argentina; 2021; 187-187
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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