Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism

Autores
Pascual, Ana Clara; Gaveglio, Virginia Lucía; Giusto, Norma Maria; Pasquaré, Susana Juana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Among the multiple functions of 2-arachidonoylglycerol (2-AG) in the central nervous system, we can highlight its role as neuroprotective molecule. We have previously demonstrated a deregulation in 2-AG hydrolysis in an in vitro model of Alzheimer´s disease (AD), in rat cerebral cortex synaptosomes (Syn). The aim of the present study was to analyze the effect of βA oligomers (mimicking early AD stage) and fibrils (mimicking late AD stage) in Syn, and also to evaluate 2-AG synthesis by diacylglycerol lipase (DAGL) and lysophosphatidate phosphohydrolase (LPAase) in these AD models. Syn were isolated by differential centrifugation, purified in ficoll gradients, and incubated with different βA peptide conformations. LPAase and DAGL activities were assayed using radiolabeled substrates. We observed that βA oligomers disrupted synaptosomal membranes while fibrils not only caused synaptosome aggregation but also showed membrane damage probably exerted by oligomeric like structures. Also, similarly to 2-AG hydrolysis, its synthesis is differentially modulated in early and late AD stages. Whereas oligomers decreased DAGL activity, fibrils increased both LPAase and DAGL activities. Our results show important differences in early and late AD stages in 2-AG metabolism that could be partially responsible for the neurodegeneration observed in this pathology.
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
BETA-AMYLOID
SYNAPTOSOMAL STRUCTURE
2-ARACHIDONOYLGLYCEROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245455
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/245455
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol MetabolismPascual, Ana ClaraGaveglio, Virginia LucíaGiusto, Norma MariaPasquaré, Susana JuanaBETA-AMYLOIDSYNAPTOSOMAL STRUCTURE2-ARACHIDONOYLGLYCEROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Among the multiple functions of 2-arachidonoylglycerol (2-AG) in the central nervous system, we can highlight its role as neuroprotective molecule. We have previously demonstrated a deregulation in 2-AG hydrolysis in an in vitro model of Alzheimer´s disease (AD), in rat cerebral cortex synaptosomes (Syn). The aim of the present study was to analyze the effect of βA oligomers (mimicking early AD stage) and fibrils (mimicking late AD stage) in Syn, and also to evaluate 2-AG synthesis by diacylglycerol lipase (DAGL) and lysophosphatidate phosphohydrolase (LPAase) in these AD models. Syn were isolated by differential centrifugation, purified in ficoll gradients, and incubated with different βA peptide conformations. LPAase and DAGL activities were assayed using radiolabeled substrates. We observed that βA oligomers disrupted synaptosomal membranes while fibrils not only caused synaptosome aggregation but also showed membrane damage probably exerted by oligomeric like structures. Also, similarly to 2-AG hydrolysis, its synthesis is differentially modulated in early and late AD stages. Whereas oligomers decreased DAGL activity, fibrils increased both LPAase and DAGL activities. Our results show important differences in early and late AD stages in 2-AG metabolism that could be partially responsible for the neurodegeneration observed in this pathology.Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularMar del PlataArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245455Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 127-1270327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:13Zoai:ri.conicet.gov.ar:11336/245455instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:13.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
title Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
spellingShingle Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
Pascual, Ana Clara
BETA-AMYLOID
SYNAPTOSOMAL STRUCTURE
2-ARACHIDONOYLGLYCEROL
title_short Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
title_full Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
title_fullStr Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
title_full_unstemmed Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
title_sort Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism
dc.creator.none.fl_str_mv Pascual, Ana Clara
Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author Pascual, Ana Clara
author_facet Pascual, Ana Clara
Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author_role author
author2 Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author2_role author
author
author
dc.subject.none.fl_str_mv BETA-AMYLOID
SYNAPTOSOMAL STRUCTURE
2-ARACHIDONOYLGLYCEROL
topic BETA-AMYLOID
SYNAPTOSOMAL STRUCTURE
2-ARACHIDONOYLGLYCEROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Among the multiple functions of 2-arachidonoylglycerol (2-AG) in the central nervous system, we can highlight its role as neuroprotective molecule. We have previously demonstrated a deregulation in 2-AG hydrolysis in an in vitro model of Alzheimer´s disease (AD), in rat cerebral cortex synaptosomes (Syn). The aim of the present study was to analyze the effect of βA oligomers (mimicking early AD stage) and fibrils (mimicking late AD stage) in Syn, and also to evaluate 2-AG synthesis by diacylglycerol lipase (DAGL) and lysophosphatidate phosphohydrolase (LPAase) in these AD models. Syn were isolated by differential centrifugation, purified in ficoll gradients, and incubated with different βA peptide conformations. LPAase and DAGL activities were assayed using radiolabeled substrates. We observed that βA oligomers disrupted synaptosomal membranes while fibrils not only caused synaptosome aggregation but also showed membrane damage probably exerted by oligomeric like structures. Also, similarly to 2-AG hydrolysis, its synthesis is differentially modulated in early and late AD stages. Whereas oligomers decreased DAGL activity, fibrils increased both LPAase and DAGL activities. Our results show important differences in early and late AD stages in 2-AG metabolism that could be partially responsible for the neurodegeneration observed in this pathology.
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Among the multiple functions of 2-arachidonoylglycerol (2-AG) in the central nervous system, we can highlight its role as neuroprotective molecule. We have previously demonstrated a deregulation in 2-AG hydrolysis in an in vitro model of Alzheimer´s disease (AD), in rat cerebral cortex synaptosomes (Syn). The aim of the present study was to analyze the effect of βA oligomers (mimicking early AD stage) and fibrils (mimicking late AD stage) in Syn, and also to evaluate 2-AG synthesis by diacylglycerol lipase (DAGL) and lysophosphatidate phosphohydrolase (LPAase) in these AD models. Syn were isolated by differential centrifugation, purified in ficoll gradients, and incubated with different βA peptide conformations. LPAase and DAGL activities were assayed using radiolabeled substrates. We observed that βA oligomers disrupted synaptosomal membranes while fibrils not only caused synaptosome aggregation but also showed membrane damage probably exerted by oligomeric like structures. Also, similarly to 2-AG hydrolysis, its synthesis is differentially modulated in early and late AD stages. Whereas oligomers decreased DAGL activity, fibrils increased both LPAase and DAGL activities. Our results show important differences in early and late AD stages in 2-AG metabolism that could be partially responsible for the neurodegeneration observed in this pathology.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245455
Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 127-127
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245455
identifier_str_mv Influence Of Β-Amyloid Assembly On Synaptosomal Structure And 2-Arachidonoylglycerol Metabolism; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 127-127
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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