Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction

Autores
Gómez Pelaytay, Jessica Belén; Simirgiotis, Mario J.; Lima, Beatriz Viviana; Paredes, Jesica Daniela; Villegas Gabutti, Carlos Mauricio; Gamarra Luques, Carlos Diego; Bórquez, Jorge; Luna, Lorena Celina; Wendel, Graciela Haydée; Maria, Alejandra Olivia Margarita; Feresin, Gabriela Egly; Tapia, Alejandro
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.
Fil: Gómez Pelaytay, Jessica Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Simirgiotis, Mario J.. Universidad Austral de Chile; Chile
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Paredes, Jesica Daniela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Villegas Gabutti, Carlos Mauricio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Gamarra Luques, Carlos Diego. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bórquez, Jorge. Universidad de Antofagasta; Chile
Fil: Luna, Lorena Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wendel, Graciela Haydée. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Maria, Alejandra Olivia Margarita. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Materia
ARGENTINEAN PLANTS
CINNAMIC ACIDS DERIVATIVES
FLAVONOIDS
UHPLC ORBITRAP (Q-OT)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147230
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147230
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoctionGómez Pelaytay, Jessica BelénSimirgiotis, Mario J.Lima, Beatriz VivianaParedes, Jesica DanielaVillegas Gabutti, Carlos MauricioGamarra Luques, Carlos DiegoBórquez, JorgeLuna, Lorena CelinaWendel, Graciela HaydéeMaria, Alejandra Olivia MargaritaFeresin, Gabriela EglyTapia, AlejandroARGENTINEAN PLANTSCINNAMIC ACIDS DERIVATIVESFLAVONOIDSUHPLC ORBITRAP (Q-OT)https://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.Fil: Gómez Pelaytay, Jessica Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Simirgiotis, Mario J.. Universidad Austral de Chile; ChileFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Paredes, Jesica Daniela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; ArgentinaFil: Villegas Gabutti, Carlos Mauricio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Gamarra Luques, Carlos Diego. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bórquez, Jorge. Universidad de Antofagasta; ChileFil: Luna, Lorena Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wendel, Graciela Haydée. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; ArgentinaFil: Maria, Alejandra Olivia Margarita. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaMolecular Diversity Preservation International2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147230Gómez Pelaytay, Jessica Belén; Simirgiotis, Mario J.; Lima, Beatriz Viviana; Paredes, Jesica Daniela; Villegas Gabutti, Carlos Mauricio; et al.; Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction; Molecular Diversity Preservation International; Molecules; 24; 6; 3-2019; 1-161420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/24/6/1085info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules24061085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:57Zoai:ri.conicet.gov.ar:11336/147230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:57.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
title Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
spellingShingle Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
Gómez Pelaytay, Jessica Belén
ARGENTINEAN PLANTS
CINNAMIC ACIDS DERIVATIVES
FLAVONOIDS
UHPLC ORBITRAP (Q-OT)
title_short Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
title_full Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
title_fullStr Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
title_full_unstemmed Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
title_sort Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction
dc.creator.none.fl_str_mv Gómez Pelaytay, Jessica Belén
Simirgiotis, Mario J.
Lima, Beatriz Viviana
Paredes, Jesica Daniela
Villegas Gabutti, Carlos Mauricio
Gamarra Luques, Carlos Diego
Bórquez, Jorge
Luna, Lorena Celina
Wendel, Graciela Haydée
Maria, Alejandra Olivia Margarita
Feresin, Gabriela Egly
Tapia, Alejandro
author Gómez Pelaytay, Jessica Belén
author_facet Gómez Pelaytay, Jessica Belén
Simirgiotis, Mario J.
Lima, Beatriz Viviana
Paredes, Jesica Daniela
Villegas Gabutti, Carlos Mauricio
Gamarra Luques, Carlos Diego
Bórquez, Jorge
Luna, Lorena Celina
Wendel, Graciela Haydée
Maria, Alejandra Olivia Margarita
Feresin, Gabriela Egly
Tapia, Alejandro
author_role author
author2 Simirgiotis, Mario J.
Lima, Beatriz Viviana
Paredes, Jesica Daniela
Villegas Gabutti, Carlos Mauricio
Gamarra Luques, Carlos Diego
Bórquez, Jorge
Luna, Lorena Celina
Wendel, Graciela Haydée
Maria, Alejandra Olivia Margarita
Feresin, Gabriela Egly
Tapia, Alejandro
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINEAN PLANTS
CINNAMIC ACIDS DERIVATIVES
FLAVONOIDS
UHPLC ORBITRAP (Q-OT)
topic ARGENTINEAN PLANTS
CINNAMIC ACIDS DERIVATIVES
FLAVONOIDS
UHPLC ORBITRAP (Q-OT)
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.
Fil: Gómez Pelaytay, Jessica Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Simirgiotis, Mario J.. Universidad Austral de Chile; Chile
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Paredes, Jesica Daniela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Villegas Gabutti, Carlos Mauricio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Gamarra Luques, Carlos Diego. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bórquez, Jorge. Universidad de Antofagasta; Chile
Fil: Luna, Lorena Celina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wendel, Graciela Haydée. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Maria, Alejandra Olivia Margarita. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Farmacología; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
description The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147230
Gómez Pelaytay, Jessica Belén; Simirgiotis, Mario J.; Lima, Beatriz Viviana; Paredes, Jesica Daniela; Villegas Gabutti, Carlos Mauricio; et al.; Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction; Molecular Diversity Preservation International; Molecules; 24; 6; 3-2019; 1-16
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147230
identifier_str_mv Gómez Pelaytay, Jessica Belén; Simirgiotis, Mario J.; Lima, Beatriz Viviana; Paredes, Jesica Daniela; Villegas Gabutti, Carlos Mauricio; et al.; Antioxidant, gastroprotective, cytotoxic activities and uhplc pda-q orbitrap mass spectrometry identification of metabolites in baccharis grisebachii decoction; Molecular Diversity Preservation International; Molecules; 24; 6; 3-2019; 1-16
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules24061085
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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