UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argent...

Autores: Gómez, Jessica; Simirgiotis, Mario Juan; Lima, Beatriz; Gamarra Luques, Carlos Diego; Bórquez, Jorge; Caballero, Duilio; Feresin, Gabriela Egly; Tapia, Alejandro
Año de publicación: 2019
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.
Fil: Gómez, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Simirgiotis, Mario Juan. Universidad Austral de Chile. , Facultad de Ciencias. Instituto de Farmacia; Chile. Universidad Austral de Chile. Center for Interdisciplinary Studies on the Nervous System; Chile
Fil: Lima, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Bórquez, Jorge. Universidad de Antofagasta; Chile
Fil: Caballero, Duilio. Hospital Marcial Quiroga; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina
Materia: UHPL-Q-EXACTIVE FOCUS
SESQUITERPENES
PHENOLIC ACIDS
FLAVONOIDS
ANTIOXIDANTS
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/110385

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and ChileGómez, JessicaSimirgiotis, Mario JuanLima, BeatrizGamarra Luques, Carlos DiegoBórquez, JorgeCaballero, DuilioFeresin, Gabriela EglyTapia, AlejandroUHPL-Q-EXACTIVE FOCUSSESQUITERPENESPHENOLIC ACIDSFLAVONOIDSANTIOXIDANTShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.Fil: Gómez, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Simirgiotis, Mario Juan. Universidad Austral de Chile. , Facultad de Ciencias. Instituto de Farmacia; Chile. Universidad Austral de Chile. Center for Interdisciplinary Studies on the Nervous System; ChileFil: Lima, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Bórquez, Jorge. Universidad de Antofagasta; ChileFil: Caballero, Duilio. Hospital Marcial Quiroga; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería; ArgentinaMultidisciplinary Digital Publishing Institute2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110385Gómez, Jessica; Simirgiotis, Mario Juan; Lima, Beatriz; Gamarra Luques, Carlos Diego; Bórquez, Jorge; et al.; UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile; Multidisciplinary Digital Publishing Institute; Antioxidants; 8; 12; 11-2019; 1-162076-3921CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/8/12/593info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox8120593info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:40Zoai:ri.conicet.gov.ar:11336/110385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:40.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
title	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
spellingShingle	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile Gómez, Jessica UHPL-Q-EXACTIVE FOCUS SESQUITERPENES PHENOLIC ACIDS FLAVONOIDS ANTIOXIDANTS
title_short	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
title_full	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
title_fullStr	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
title_full_unstemmed	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
title_sort	UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile
dc.creator.none.fl_str_mv	Gómez, Jessica Simirgiotis, Mario Juan Lima, Beatriz Gamarra Luques, Carlos Diego Bórquez, Jorge Caballero, Duilio Feresin, Gabriela Egly Tapia, Alejandro
author	Gómez, Jessica
author_facet	Gómez, Jessica Simirgiotis, Mario Juan Lima, Beatriz Gamarra Luques, Carlos Diego Bórquez, Jorge Caballero, Duilio Feresin, Gabriela Egly Tapia, Alejandro
author_role	author
author2	Simirgiotis, Mario Juan Lima, Beatriz Gamarra Luques, Carlos Diego Bórquez, Jorge Caballero, Duilio Feresin, Gabriela Egly Tapia, Alejandro
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	UHPL-Q-EXACTIVE FOCUS SESQUITERPENES PHENOLIC ACIDS FLAVONOIDS ANTIOXIDANTS
topic	UHPL-Q-EXACTIVE FOCUS SESQUITERPENES PHENOLIC ACIDS FLAVONOIDS ANTIOXIDANTS
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health. Fil: Gómez, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Simirgiotis, Mario Juan. Universidad Austral de Chile. , Facultad de Ciencias. Instituto de Farmacia; Chile. Universidad Austral de Chile. Center for Interdisciplinary Studies on the Nervous System; Chile Fil: Lima, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Bórquez, Jorge. Universidad de Antofagasta; Chile Fil: Caballero, Duilio. Hospital Marcial Quiroga; Argentina Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentina
description	The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.
publishDate	2019
dc.date.none.fl_str_mv	2019-11
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/110385 Gómez, Jessica; Simirgiotis, Mario Juan; Lima, Beatriz; Gamarra Luques, Carlos Diego; Bórquez, Jorge; et al.; UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile; Multidisciplinary Digital Publishing Institute; Antioxidants; 8; 12; 11-2019; 1-16 2076-3921 CONICET Digital CONICET
url	http://hdl.handle.net/11336/110385
identifier_str_mv	Gómez, Jessica; Simirgiotis, Mario Juan; Lima, Beatriz; Gamarra Luques, Carlos Diego; Bórquez, Jorge; et al.; UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile; Multidisciplinary Digital Publishing Institute; Antioxidants; 8; 12; 11-2019; 1-16 2076-3921 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/8/12/593 info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox8120593
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	12.993085

UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argent...

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