Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats

Autores
Sosa, Gustavo Adolfo; Quiroga, María Florencia; Roux, Maria
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4+, CD8α+, and TCRαβ+ lymphocytes but TCRγδ+ cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest® and WinMDI 2.9. In C60, the predominant population was CD4+TCRαβ+, which was significantly diminished in the R21 group. However, CD8α+, the majority expressing CD8αβ, and TCRγδ+ cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.
Fil: Sosa, Gustavo Adolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Quiroga, María Florencia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roux, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Malnutrition; Wistar rats
NALT;
TCRab
Secondary immunodeficiency
CD4
TCRgd
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113752
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113752
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar ratsSosa, Gustavo AdolfoQuiroga, María FlorenciaRoux, MariaMalnutrition; Wistar ratsNALT;TCRabSecondary immunodeficiencyCD4TCRgdhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4+, CD8α+, and TCRαβ+ lymphocytes but TCRγδ+ cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest® and WinMDI 2.9. In C60, the predominant population was CD4+TCRαβ+, which was significantly diminished in the R21 group. However, CD8α+, the majority expressing CD8αβ, and TCRγδ+ cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.Fil: Sosa, Gustavo Adolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Quiroga, María Florencia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roux, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Gmbh2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113752Sosa, Gustavo Adolfo; Quiroga, María Florencia; Roux, Maria; Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats; Elsevier Gmbh; Immunobiology; 214; 5; 5-2009; 384-3910171-2985CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0171298508001332info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imbio.2008.10.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:32Zoai:ri.conicet.gov.ar:11336/113752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:32.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
title Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
spellingShingle Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
Sosa, Gustavo Adolfo
Malnutrition; Wistar rats
NALT;
TCRab
Secondary immunodeficiency
CD4
TCRgd
title_short Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
title_full Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
title_fullStr Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
title_full_unstemmed Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
title_sort Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats
dc.creator.none.fl_str_mv Sosa, Gustavo Adolfo
Quiroga, María Florencia
Roux, Maria
author Sosa, Gustavo Adolfo
author_facet Sosa, Gustavo Adolfo
Quiroga, María Florencia
Roux, Maria
author_role author
author2 Quiroga, María Florencia
Roux, Maria
author2_role author
author
dc.subject.none.fl_str_mv Malnutrition; Wistar rats
NALT;
TCRab
Secondary immunodeficiency
CD4
TCRgd
topic Malnutrition; Wistar rats
NALT;
TCRab
Secondary immunodeficiency
CD4
TCRgd
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4+, CD8α+, and TCRαβ+ lymphocytes but TCRγδ+ cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest® and WinMDI 2.9. In C60, the predominant population was CD4+TCRαβ+, which was significantly diminished in the R21 group. However, CD8α+, the majority expressing CD8αβ, and TCRγδ+ cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.
Fil: Sosa, Gustavo Adolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Quiroga, María Florencia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roux, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4+, CD8α+, and TCRαβ+ lymphocytes but TCRγδ+ cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest® and WinMDI 2.9. In C60, the predominant population was CD4+TCRαβ+, which was significantly diminished in the R21 group. However, CD8α+, the majority expressing CD8αβ, and TCRγδ+ cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113752
Sosa, Gustavo Adolfo; Quiroga, María Florencia; Roux, Maria; Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats; Elsevier Gmbh; Immunobiology; 214; 5; 5-2009; 384-391
0171-2985
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113752
identifier_str_mv Sosa, Gustavo Adolfo; Quiroga, María Florencia; Roux, Maria; Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats; Elsevier Gmbh; Immunobiology; 214; 5; 5-2009; 384-391
0171-2985
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0171298508001332
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imbio.2008.10.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269805702283264
score 13.13397

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