Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice

Autores
Ivir, Héctor Maximiliano; Vasile, Brenda Estefania; Gutiérrez, Florencia; Alvarez Villamil, Eduardo Gaspar; Lorenzo Pisarello, Maria Jose; Salva, Maria Susana; Alvarez, Susana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The nasal priming of malnourished mice with the peptidoglycan (PG) of Lacticaseibacillus rhamnosus CRL1505 (Lr) is as effective as viable strain for improving systemic and respiratory immune response against Streptococcus pneumoniae (Sp). But the impact of these treatments on mucosa-associated lymphoid tissue is unknown. Hence, the effect of nasal administration of Lr or PG on the innate immune response of nasopharynx-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in malnourished mice under repletion treatments was evaluated. Weaned Swiss mice were malnourished with a protein-free diet (PFD) for 21d. Malnourished mice received a balanced conventional diet (BCD) during 7d (BCD group) or BCD with nasal supplementation with Lr (108 cells/mouse/d) or PG (8 µg/mouse/d) during the last 2d of treatment (Lr or PG groups). Malnourished control mice (MNC) received PFD while the well-nourished control group (WNC) consumed BCD. On d8, all groups were infected with Sp (107 cells/mouse). Before infection, MNC showed a significant decrease of the total cells, T and B lymphocytes in NALT and CLN as well as the macrophages, myeloid and dendritic cells in NALT. In addition, the MNC showed an increase of NALT Gr-1+ cells % and the microbial load in nasal washes. BCD treatment was not able to normalize these parameters. However, the Lr and PG groups improved the total cells, B and T cells counts in NALT and CLN. Challenge with Sp increased the numbers of neutrophils and macrophages and TNF-α, INF-γ, IL-6, IL17 and IL-10 levels in nasal washes. The values were lower in MNC than in WNC. However, unlike the BDC group, Lr and PG groups showed values of NALT phagocytes, and lymphocytes, T CD4+ cells in CLN and NALT similar to WNC mice. Moreover, IL-6, INF-γ and TNF-α levels in nasal washes were higher in Lr or PG groups. NALT is a target for postbiotics administration to improve respiratory immunity in immunocompromised malnourished hosts.
Fil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez Villamil, Eduardo Gaspar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lorenzo Pisarello, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
POSTBIOTICS
NALT
NASAL PRIMING
RESPIRATORY INFECTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213359
Acceder
id CONICETDig_00fa1e261543e26575120002e6cf871d
oai_identifier_str oai:ri.conicet.gov.ar:11336/213359
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished miceIvir, Héctor MaximilianoVasile, Brenda EstefaniaGutiérrez, FlorenciaAlvarez Villamil, Eduardo GasparLorenzo Pisarello, Maria JoseSalva, Maria SusanaAlvarez, SusanaPOSTBIOTICSNALTNASAL PRIMINGRESPIRATORY INFECTIONShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The nasal priming of malnourished mice with the peptidoglycan (PG) of Lacticaseibacillus rhamnosus CRL1505 (Lr) is as effective as viable strain for improving systemic and respiratory immune response against Streptococcus pneumoniae (Sp). But the impact of these treatments on mucosa-associated lymphoid tissue is unknown. Hence, the effect of nasal administration of Lr or PG on the innate immune response of nasopharynx-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in malnourished mice under repletion treatments was evaluated. Weaned Swiss mice were malnourished with a protein-free diet (PFD) for 21d. Malnourished mice received a balanced conventional diet (BCD) during 7d (BCD group) or BCD with nasal supplementation with Lr (108 cells/mouse/d) or PG (8 µg/mouse/d) during the last 2d of treatment (Lr or PG groups). Malnourished control mice (MNC) received PFD while the well-nourished control group (WNC) consumed BCD. On d8, all groups were infected with Sp (107 cells/mouse). Before infection, MNC showed a significant decrease of the total cells, T and B lymphocytes in NALT and CLN as well as the macrophages, myeloid and dendritic cells in NALT. In addition, the MNC showed an increase of NALT Gr-1+ cells % and the microbial load in nasal washes. BCD treatment was not able to normalize these parameters. However, the Lr and PG groups improved the total cells, B and T cells counts in NALT and CLN. Challenge with Sp increased the numbers of neutrophils and macrophages and TNF-α, INF-γ, IL-6, IL17 and IL-10 levels in nasal washes. The values were lower in MNC than in WNC. However, unlike the BDC group, Lr and PG groups showed values of NALT phagocytes, and lymphocytes, T CD4+ cells in CLN and NALT similar to WNC mice. Moreover, IL-6, INF-γ and TNF-α levels in nasal washes were higher in Lr or PG groups. NALT is a target for postbiotics administration to improve respiratory immunity in immunocompromised malnourished hosts.Fil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez Villamil, Eduardo Gaspar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Lorenzo Pisarello, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaLXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213359Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 179-1790025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:23Zoai:ri.conicet.gov.ar:11336/213359instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:23.649CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
title Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
spellingShingle Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
Ivir, Héctor Maximiliano
POSTBIOTICS
NALT
NASAL PRIMING
RESPIRATORY INFECTIONS
title_short Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
title_full Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
title_fullStr Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
title_full_unstemmed Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
title_sort Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice
dc.creator.none.fl_str_mv Ivir, Héctor Maximiliano
Vasile, Brenda Estefania
Gutiérrez, Florencia
Alvarez Villamil, Eduardo Gaspar
Lorenzo Pisarello, Maria Jose
Salva, Maria Susana
Alvarez, Susana
author Ivir, Héctor Maximiliano
author_facet Ivir, Héctor Maximiliano
Vasile, Brenda Estefania
Gutiérrez, Florencia
Alvarez Villamil, Eduardo Gaspar
Lorenzo Pisarello, Maria Jose
Salva, Maria Susana
Alvarez, Susana
author_role author
author2 Vasile, Brenda Estefania
Gutiérrez, Florencia
Alvarez Villamil, Eduardo Gaspar
Lorenzo Pisarello, Maria Jose
Salva, Maria Susana
Alvarez, Susana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv POSTBIOTICS
NALT
NASAL PRIMING
RESPIRATORY INFECTIONS
topic POSTBIOTICS
NALT
NASAL PRIMING
RESPIRATORY INFECTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The nasal priming of malnourished mice with the peptidoglycan (PG) of Lacticaseibacillus rhamnosus CRL1505 (Lr) is as effective as viable strain for improving systemic and respiratory immune response against Streptococcus pneumoniae (Sp). But the impact of these treatments on mucosa-associated lymphoid tissue is unknown. Hence, the effect of nasal administration of Lr or PG on the innate immune response of nasopharynx-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in malnourished mice under repletion treatments was evaluated. Weaned Swiss mice were malnourished with a protein-free diet (PFD) for 21d. Malnourished mice received a balanced conventional diet (BCD) during 7d (BCD group) or BCD with nasal supplementation with Lr (108 cells/mouse/d) or PG (8 µg/mouse/d) during the last 2d of treatment (Lr or PG groups). Malnourished control mice (MNC) received PFD while the well-nourished control group (WNC) consumed BCD. On d8, all groups were infected with Sp (107 cells/mouse). Before infection, MNC showed a significant decrease of the total cells, T and B lymphocytes in NALT and CLN as well as the macrophages, myeloid and dendritic cells in NALT. In addition, the MNC showed an increase of NALT Gr-1+ cells % and the microbial load in nasal washes. BCD treatment was not able to normalize these parameters. However, the Lr and PG groups improved the total cells, B and T cells counts in NALT and CLN. Challenge with Sp increased the numbers of neutrophils and macrophages and TNF-α, INF-γ, IL-6, IL17 and IL-10 levels in nasal washes. The values were lower in MNC than in WNC. However, unlike the BDC group, Lr and PG groups showed values of NALT phagocytes, and lymphocytes, T CD4+ cells in CLN and NALT similar to WNC mice. Moreover, IL-6, INF-γ and TNF-α levels in nasal washes were higher in Lr or PG groups. NALT is a target for postbiotics administration to improve respiratory immunity in immunocompromised malnourished hosts.
Fil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez Villamil, Eduardo Gaspar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lorenzo Pisarello, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description The nasal priming of malnourished mice with the peptidoglycan (PG) of Lacticaseibacillus rhamnosus CRL1505 (Lr) is as effective as viable strain for improving systemic and respiratory immune response against Streptococcus pneumoniae (Sp). But the impact of these treatments on mucosa-associated lymphoid tissue is unknown. Hence, the effect of nasal administration of Lr or PG on the innate immune response of nasopharynx-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in malnourished mice under repletion treatments was evaluated. Weaned Swiss mice were malnourished with a protein-free diet (PFD) for 21d. Malnourished mice received a balanced conventional diet (BCD) during 7d (BCD group) or BCD with nasal supplementation with Lr (108 cells/mouse/d) or PG (8 µg/mouse/d) during the last 2d of treatment (Lr or PG groups). Malnourished control mice (MNC) received PFD while the well-nourished control group (WNC) consumed BCD. On d8, all groups were infected with Sp (107 cells/mouse). Before infection, MNC showed a significant decrease of the total cells, T and B lymphocytes in NALT and CLN as well as the macrophages, myeloid and dendritic cells in NALT. In addition, the MNC showed an increase of NALT Gr-1+ cells % and the microbial load in nasal washes. BCD treatment was not able to normalize these parameters. However, the Lr and PG groups improved the total cells, B and T cells counts in NALT and CLN. Challenge with Sp increased the numbers of neutrophils and macrophages and TNF-α, INF-γ, IL-6, IL17 and IL-10 levels in nasal washes. The values were lower in MNC than in WNC. However, unlike the BDC group, Lr and PG groups showed values of NALT phagocytes, and lymphocytes, T CD4+ cells in CLN and NALT similar to WNC mice. Moreover, IL-6, INF-γ and TNF-α levels in nasal washes were higher in Lr or PG groups. NALT is a target for postbiotics administration to improve respiratory immunity in immunocompromised malnourished hosts.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213359
Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 179-179
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213359
identifier_str_mv Postbiotic nasal priming improves of innate immune response in respiratory mucosa-associated lymphoid tissue in malnourished mice; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 179-179
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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