Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis

Autores
Lizarraga, Maria Alfonsina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Laboratory animals are essential in research. Genetic and health quality control enables reliable and reproducible experimentaldata. A very common disease in rodent colony facilities, is a parasitosis caused by the etiological agent, species: Syphacia muris, whichbelongs to the filo: Nematoda, to the family: Oxyuridae, is an intestinal endoparasite. The life cycle of this parasite is direct, (there isno intermediate host) has a cycle of 7 to 9 days. Syphacia muris Las infections can alter a humoral host´s response to parasitic antigenic stimuli, and cause a Th2-like immune response with the production of elevated cytokines and induce an autoimmunity mediatedby Th2 [1]. Therefore a study was carried out in colonies of rats Wistar strain (adult males and females) of a conventional animal carefacility of faculty of medicine of the University of Buenos Aires. A total of 36, 32 y 30 rats were tested. Random samples were takenfrom animals, by Graham´s method. Treatments were: treatment A: Ivermectin administered subcutaneously according to the weightof the animal, every 15 days. Treatment B: Ivermectin administered subcutaneously according to the weight of the animal, each week.In treatment C: Ivermectin was administered orally in drinking water at a concentration of 2ml ivermectin in 500ml of filtered water,for four days and allowed to rest for 3 days, this treatment was repeated for three weeks. Together, spray cage spraying containing anivermectin solution was performed: 1ml in 250 ml of filtered water. The calculated prevalence rate for each treatment was: treatmentA: in the first dose 50% and in the second dose of 75%. In treatment B: a 75% rate was obtained at the first and second doses of 80%.In treatment C: it was 0% in the first and second doses. These results enabled the finding of adequate and effective treatment for theeradication of oxyides. A parasitosis very common and common in all biotheres, but so untreated. Therefore, this work demonstratedthat it is possible to be treated, eradicated and controlled to avoid interference and alter experimental responses. However, ensuremore reliable results by controlling some variables, such as the health status of animals. Obtaining a good experimental model, applying the uses of 3Rs [10], in terms of reduction when treating infected animals and avoiding the subsequent use of other animalsand in refinement when ensuring the welfare of animals.
Fil: Lizarraga, Maria Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
SYPHACIA MURIS
WISTAR RATS
IVERMECTIN
CONVENTIONAL ANIMAL CARE FACILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158092

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oai_identifier_str oai:ri.conicet.gov.ar:11336/158092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this ParasitosisLizarraga, Maria AlfonsinaSYPHACIA MURISWISTAR RATSIVERMECTINCONVENTIONAL ANIMAL CARE FACILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Laboratory animals are essential in research. Genetic and health quality control enables reliable and reproducible experimentaldata. A very common disease in rodent colony facilities, is a parasitosis caused by the etiological agent, species: Syphacia muris, whichbelongs to the filo: Nematoda, to the family: Oxyuridae, is an intestinal endoparasite. The life cycle of this parasite is direct, (there isno intermediate host) has a cycle of 7 to 9 days. Syphacia muris Las infections can alter a humoral host´s response to parasitic antigenic stimuli, and cause a Th2-like immune response with the production of elevated cytokines and induce an autoimmunity mediatedby Th2 [1]. Therefore a study was carried out in colonies of rats Wistar strain (adult males and females) of a conventional animal carefacility of faculty of medicine of the University of Buenos Aires. A total of 36, 32 y 30 rats were tested. Random samples were takenfrom animals, by Graham´s method. Treatments were: treatment A: Ivermectin administered subcutaneously according to the weightof the animal, every 15 days. Treatment B: Ivermectin administered subcutaneously according to the weight of the animal, each week.In treatment C: Ivermectin was administered orally in drinking water at a concentration of 2ml ivermectin in 500ml of filtered water,for four days and allowed to rest for 3 days, this treatment was repeated for three weeks. Together, spray cage spraying containing anivermectin solution was performed: 1ml in 250 ml of filtered water. The calculated prevalence rate for each treatment was: treatmentA: in the first dose 50% and in the second dose of 75%. In treatment B: a 75% rate was obtained at the first and second doses of 80%.In treatment C: it was 0% in the first and second doses. These results enabled the finding of adequate and effective treatment for theeradication of oxyides. A parasitosis very common and common in all biotheres, but so untreated. Therefore, this work demonstratedthat it is possible to be treated, eradicated and controlled to avoid interference and alter experimental responses. However, ensuremore reliable results by controlling some variables, such as the health status of animals. Obtaining a good experimental model, applying the uses of 3Rs [10], in terms of reduction when treating infected animals and avoiding the subsequent use of other animalsand in refinement when ensuring the welfare of animals.Fil: Lizarraga, Maria Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaActa Scientific Microbiology2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158092Lizarraga, Maria Alfonsina; Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis; Acta Scientific Microbiology; Acta Scientific Microbiology; 3; 9; 8-2020; 69-722581-3226CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://actascientific.com/ASMI/pdf/ASMI-03-0679.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.31080/ASMI.2020.03.0679info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:36Zoai:ri.conicet.gov.ar:11336/158092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:37.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
title Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
spellingShingle Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
Lizarraga, Maria Alfonsina
SYPHACIA MURIS
WISTAR RATS
IVERMECTIN
CONVENTIONAL ANIMAL CARE FACILITY
title_short Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
title_full Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
title_fullStr Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
title_full_unstemmed Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
title_sort Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis
dc.creator.none.fl_str_mv Lizarraga, Maria Alfonsina
author Lizarraga, Maria Alfonsina
author_facet Lizarraga, Maria Alfonsina
author_role author
dc.subject.none.fl_str_mv SYPHACIA MURIS
WISTAR RATS
IVERMECTIN
CONVENTIONAL ANIMAL CARE FACILITY
topic SYPHACIA MURIS
WISTAR RATS
IVERMECTIN
CONVENTIONAL ANIMAL CARE FACILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Laboratory animals are essential in research. Genetic and health quality control enables reliable and reproducible experimentaldata. A very common disease in rodent colony facilities, is a parasitosis caused by the etiological agent, species: Syphacia muris, whichbelongs to the filo: Nematoda, to the family: Oxyuridae, is an intestinal endoparasite. The life cycle of this parasite is direct, (there isno intermediate host) has a cycle of 7 to 9 days. Syphacia muris Las infections can alter a humoral host´s response to parasitic antigenic stimuli, and cause a Th2-like immune response with the production of elevated cytokines and induce an autoimmunity mediatedby Th2 [1]. Therefore a study was carried out in colonies of rats Wistar strain (adult males and females) of a conventional animal carefacility of faculty of medicine of the University of Buenos Aires. A total of 36, 32 y 30 rats were tested. Random samples were takenfrom animals, by Graham´s method. Treatments were: treatment A: Ivermectin administered subcutaneously according to the weightof the animal, every 15 days. Treatment B: Ivermectin administered subcutaneously according to the weight of the animal, each week.In treatment C: Ivermectin was administered orally in drinking water at a concentration of 2ml ivermectin in 500ml of filtered water,for four days and allowed to rest for 3 days, this treatment was repeated for three weeks. Together, spray cage spraying containing anivermectin solution was performed: 1ml in 250 ml of filtered water. The calculated prevalence rate for each treatment was: treatmentA: in the first dose 50% and in the second dose of 75%. In treatment B: a 75% rate was obtained at the first and second doses of 80%.In treatment C: it was 0% in the first and second doses. These results enabled the finding of adequate and effective treatment for theeradication of oxyides. A parasitosis very common and common in all biotheres, but so untreated. Therefore, this work demonstratedthat it is possible to be treated, eradicated and controlled to avoid interference and alter experimental responses. However, ensuremore reliable results by controlling some variables, such as the health status of animals. Obtaining a good experimental model, applying the uses of 3Rs [10], in terms of reduction when treating infected animals and avoiding the subsequent use of other animalsand in refinement when ensuring the welfare of animals.
Fil: Lizarraga, Maria Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Laboratory animals are essential in research. Genetic and health quality control enables reliable and reproducible experimentaldata. A very common disease in rodent colony facilities, is a parasitosis caused by the etiological agent, species: Syphacia muris, whichbelongs to the filo: Nematoda, to the family: Oxyuridae, is an intestinal endoparasite. The life cycle of this parasite is direct, (there isno intermediate host) has a cycle of 7 to 9 days. Syphacia muris Las infections can alter a humoral host´s response to parasitic antigenic stimuli, and cause a Th2-like immune response with the production of elevated cytokines and induce an autoimmunity mediatedby Th2 [1]. Therefore a study was carried out in colonies of rats Wistar strain (adult males and females) of a conventional animal carefacility of faculty of medicine of the University of Buenos Aires. A total of 36, 32 y 30 rats were tested. Random samples were takenfrom animals, by Graham´s method. Treatments were: treatment A: Ivermectin administered subcutaneously according to the weightof the animal, every 15 days. Treatment B: Ivermectin administered subcutaneously according to the weight of the animal, each week.In treatment C: Ivermectin was administered orally in drinking water at a concentration of 2ml ivermectin in 500ml of filtered water,for four days and allowed to rest for 3 days, this treatment was repeated for three weeks. Together, spray cage spraying containing anivermectin solution was performed: 1ml in 250 ml of filtered water. The calculated prevalence rate for each treatment was: treatmentA: in the first dose 50% and in the second dose of 75%. In treatment B: a 75% rate was obtained at the first and second doses of 80%.In treatment C: it was 0% in the first and second doses. These results enabled the finding of adequate and effective treatment for theeradication of oxyides. A parasitosis very common and common in all biotheres, but so untreated. Therefore, this work demonstratedthat it is possible to be treated, eradicated and controlled to avoid interference and alter experimental responses. However, ensuremore reliable results by controlling some variables, such as the health status of animals. Obtaining a good experimental model, applying the uses of 3Rs [10], in terms of reduction when treating infected animals and avoiding the subsequent use of other animalsand in refinement when ensuring the welfare of animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158092
Lizarraga, Maria Alfonsina; Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis; Acta Scientific Microbiology; Acta Scientific Microbiology; 3; 9; 8-2020; 69-72
2581-3226
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158092
identifier_str_mv Lizarraga, Maria Alfonsina; Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis; Acta Scientific Microbiology; Acta Scientific Microbiology; 3; 9; 8-2020; 69-72
2581-3226
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://actascientific.com/ASMI/pdf/ASMI-03-0679.pdf
info:eu-repo/semantics/altIdentifier/doi/10.31080/ASMI.2020.03.0679
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Acta Scientific Microbiology
publisher.none.fl_str_mv Acta Scientific Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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