Creatine supplementation in wistar rats submitted to physical tests of swimming: evaluation of the physiological effects through raman spectroscopy

• Autores: Miranda, Humberto; da Silva Novaes, Jefferson; de Souza, Renato Aparecido; Pereira Jeronimo, Diego; Ribeiro, Wellington; Gaspar Tosato, Maira; Simao, Roberto; Abrahao Martin, Airton; Marmo Moreira, Leonardo
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Creatine Supplementation; Swimming; Raman Spectroscopy; Wistar Rats; Physical Test
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Creatine is a nitrogenous organic acid that naturally occurs in vertebrates. This compound is associated to energy supply to muscles, being that its biological synthesis occurs mainly in liver and is related to the aminoacids glycine, arginine and ornitine. Several studies have shown the benefits of creatine supplementation, such as higher muscle power, increased fat-free mass, and strength in healthy subjects. Besides, it can be applied as therapeutic agent for some diseases. In this work, the physical condition of Wistar rats that received creatine supplementation is compared with individuals without any supplementation. For this evaluation, swimming tests were developed in order to elucidate the physical condition of animals in different nutritional conditions. Indeed, swimming is considered one of the most complete exercises, since the practice of this physical activity involves great increase in the aerobic and anaerobic capability. Raman spectroscopy was employed to analyse the biochemical profile of the biological tissues obtained from the animals evaluated in the experiments. The present results allow to infer that the association of these two factors, i.e., the addition of creatine supplementation together with physical activity, propitiates a completely different physiological effect when compared with the isolated action of each one of these factors (creatine supplementation and physical activity). In fact, these factors seem to act in synergy, favoring a more accentuated physiological response. In fact, the data obtained in this work denotes that the physical activity, as well as the creatine supplementation, when used independently, does not provoke a very significant physiological effect. Raman spectroscopy was able to analyze important biological indicators, such as protein concentration in blood and intensity of metabolic activity, being an interesting option to similar biophysical evaluations.
Fil: Miranda, Humberto. Universidade Federal do Rio de Janeiro; Brasil. Universidade do Vale do Paraíba; Brasil
Fil: da Silva Novaes, Jefferson. Universidade Federal do Rio de Janeiro; Brasil
Fil: de Souza, Renato Aparecido. Universidade do Vale do Paraíba; Brasil
Fil: Pereira Jeronimo, Diego. Universidade do Vale do Paraíba; Brasil
Fil: Ribeiro, Wellington. Universidade do Vale do Paraíba; Brasil
Fil: Gaspar Tosato, Maira. Universidade do Vale do Paraíba; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Simao, Roberto. Universidade Federal do Rio de Janeiro; Brasil
Fil: Abrahao Martin, Airton. Universidade do Vale do Paraíba; Brasil
Fil: Marmo Moreira, Leonardo. Universidade Federal de Sao Joao Del Rei ; Brasil