Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides

Autores
Chowdhury, Jamil; Kemppainen, Minna Johanna; Delhomme, Nicolas; Shutava, Iryna; Zhou, Jingjing; Takahashi, Junko; Pardo, Alejandro Guillermo; Lundberg-Felten, Judith
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
Fil: Chowdhury, Jamil. Universidad de Umea; Suecia
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Delhomme, Nicolas. Universidad de Umea; Suecia
Fil: Shutava, Iryna. Universidad de Umea; Suecia
Fil: Zhou, Jingjing. Universidad de Umea; Suecia
Fil: Takahashi, Junko. Universidad de Umea; Suecia
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Lundberg-Felten, Judith. Universidad de Umea; Suecia
Materia
CELL WALL
CELL-WALL REMODELLING
CELL-WALL-MODIFYING ENZYMES
ECTOMYCORRHIZA
LACCARIA BICOLOR
PECTIN
PECTIN METHYLESTERASE
POPULUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201690
Acceder
id CONICETDig_6159668b17566c2b9006b33e4929e363
oai_identifier_str oai:ri.conicet.gov.ar:11336/201690
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloidesChowdhury, JamilKemppainen, Minna JohannaDelhomme, NicolasShutava, IrynaZhou, JingjingTakahashi, JunkoPardo, Alejandro GuillermoLundberg-Felten, JudithCELL WALLCELL-WALL REMODELLINGCELL-WALL-MODIFYING ENZYMESECTOMYCORRHIZALACCARIA BICOLORPECTINPECTIN METHYLESTERASEPOPULUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.Fil: Chowdhury, Jamil. Universidad de Umea; SueciaFil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; ArgentinaFil: Delhomme, Nicolas. Universidad de Umea; SueciaFil: Shutava, Iryna. Universidad de Umea; SueciaFil: Zhou, Jingjing. Universidad de Umea; SueciaFil: Takahashi, Junko. Universidad de Umea; SueciaFil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; ArgentinaFil: Lundberg-Felten, Judith. Universidad de Umea; SueciaWiley Blackwell Publishing, Inc2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201690Chowdhury, Jamil; Kemppainen, Minna Johanna; Delhomme, Nicolas; Shutava, Iryna; Zhou, Jingjing; et al.; Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides; Wiley Blackwell Publishing, Inc; New Phytologist; 236; 2; 10-2022; 639-6550028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18358info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18358info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:22:57Zoai:ri.conicet.gov.ar:11336/201690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:22:58.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
spellingShingle Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
Chowdhury, Jamil
CELL WALL
CELL-WALL REMODELLING
CELL-WALL-MODIFYING ENZYMES
ECTOMYCORRHIZA
LACCARIA BICOLOR
PECTIN
PECTIN METHYLESTERASE
POPULUS
title_short Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_full Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_fullStr Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_full_unstemmed Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_sort Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
dc.creator.none.fl_str_mv Chowdhury, Jamil
Kemppainen, Minna Johanna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro Guillermo
Lundberg-Felten, Judith
author Chowdhury, Jamil
author_facet Chowdhury, Jamil
Kemppainen, Minna Johanna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro Guillermo
Lundberg-Felten, Judith
author_role author
author2 Kemppainen, Minna Johanna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro Guillermo
Lundberg-Felten, Judith
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL WALL
CELL-WALL REMODELLING
CELL-WALL-MODIFYING ENZYMES
ECTOMYCORRHIZA
LACCARIA BICOLOR
PECTIN
PECTIN METHYLESTERASE
POPULUS
topic CELL WALL
CELL-WALL REMODELLING
CELL-WALL-MODIFYING ENZYMES
ECTOMYCORRHIZA
LACCARIA BICOLOR
PECTIN
PECTIN METHYLESTERASE
POPULUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
Fil: Chowdhury, Jamil. Universidad de Umea; Suecia
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Delhomme, Nicolas. Universidad de Umea; Suecia
Fil: Shutava, Iryna. Universidad de Umea; Suecia
Fil: Zhou, Jingjing. Universidad de Umea; Suecia
Fil: Takahashi, Junko. Universidad de Umea; Suecia
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Lundberg-Felten, Judith. Universidad de Umea; Suecia
description The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201690
Chowdhury, Jamil; Kemppainen, Minna Johanna; Delhomme, Nicolas; Shutava, Iryna; Zhou, Jingjing; et al.; Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides; Wiley Blackwell Publishing, Inc; New Phytologist; 236; 2; 10-2022; 639-655
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201690
identifier_str_mv Chowdhury, Jamil; Kemppainen, Minna Johanna; Delhomme, Nicolas; Shutava, Iryna; Zhou, Jingjing; et al.; Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides; Wiley Blackwell Publishing, Inc; New Phytologist; 236; 2; 10-2022; 639-655
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18358
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18358
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082632852439040
score 13.22299

Publicaciones similares