Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness

Autores
Villarreal, Natalia Marina; Rosli, Hernan Guillermo; Martinez, Gustavo Adolfo; Civello, Pedro Marcos
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fleshy fruits soften during ripening mainly as a consequence of the catabolism of cell wall components. In strawberry (Fragaria × ananassa Duch), the depolymerization and solubilization of pectins increase during ripening and contribute to fruit softening. In the present paper, we report the cloning and expression analysis of two polygalacturonase (PG) putative cDNAs: FaPG1 and T-PG. The former seems to be the same sequence of previously reported PG in strawberry, while T-PG cDNA has a deletion of 85 bp that cause a frame shift and would produce an inactive protein. Measurement of total PG activity and expression of FaPG1 and T-PG were performed in strawberry cultivars with contrasting softening rates. The softest cultivar (Toyonaka) showed the higher total PG activity in all ripening stages analyzed. The analysis by RT-PCR revealed that both genes express in the three cultivars, though the expression pattern was different. In the firmer cultivars (Selva and Camarosa) the expression of T-PG was considerably higher than the expression of FaPG1, while the opposite occurred in the softest cultivar (Toyonaka). Therefore, the higher PG activity detected in Toyonaka correlates with the enhanced expression of FaPG1 gene, while the low PG activity found in the firm cultivars correlates with a higher expression of T-PG, a gene that could encode a truncated protein without PG activity. The influence of auxins on both the expression of PG genes and the total PG activity during strawberry fruit ripening was also analyzed.
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
CELL WALL
FRAGARIA
PECTIN
POLYGALACTURONASE
SOFTENING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155127
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/155127
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmnessVillarreal, Natalia MarinaRosli, Hernan GuillermoMartinez, Gustavo AdolfoCivello, Pedro MarcosCELL WALLFRAGARIAPECTINPOLYGALACTURONASESOFTENINGhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Fleshy fruits soften during ripening mainly as a consequence of the catabolism of cell wall components. In strawberry (Fragaria × ananassa Duch), the depolymerization and solubilization of pectins increase during ripening and contribute to fruit softening. In the present paper, we report the cloning and expression analysis of two polygalacturonase (PG) putative cDNAs: FaPG1 and T-PG. The former seems to be the same sequence of previously reported PG in strawberry, while T-PG cDNA has a deletion of 85 bp that cause a frame shift and would produce an inactive protein. Measurement of total PG activity and expression of FaPG1 and T-PG were performed in strawberry cultivars with contrasting softening rates. The softest cultivar (Toyonaka) showed the higher total PG activity in all ripening stages analyzed. The analysis by RT-PCR revealed that both genes express in the three cultivars, though the expression pattern was different. In the firmer cultivars (Selva and Camarosa) the expression of T-PG was considerably higher than the expression of FaPG1, while the opposite occurred in the softest cultivar (Toyonaka). Therefore, the higher PG activity detected in Toyonaka correlates with the enhanced expression of FaPG1 gene, while the low PG activity found in the firm cultivars correlates with a higher expression of T-PG, a gene that could encode a truncated protein without PG activity. The influence of auxins on both the expression of PG genes and the total PG activity during strawberry fruit ripening was also analyzed.Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaElsevier Science2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155127Villarreal, Natalia Marina; Rosli, Hernan Guillermo; Martinez, Gustavo Adolfo; Civello, Pedro Marcos; Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness; Elsevier Science; Postharvest Biology and Technology; 47; 2; 2-2008; 141-1500925-5214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2007.06.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925521407002177?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:56Zoai:ri.conicet.gov.ar:11336/155127instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:56.355CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
title Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
spellingShingle Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
Villarreal, Natalia Marina
CELL WALL
FRAGARIA
PECTIN
POLYGALACTURONASE
SOFTENING
title_short Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
title_full Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
title_fullStr Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
title_full_unstemmed Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
title_sort Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness
dc.creator.none.fl_str_mv Villarreal, Natalia Marina
Rosli, Hernan Guillermo
Martinez, Gustavo Adolfo
Civello, Pedro Marcos
author Villarreal, Natalia Marina
author_facet Villarreal, Natalia Marina
Rosli, Hernan Guillermo
Martinez, Gustavo Adolfo
Civello, Pedro Marcos
author_role author
author2 Rosli, Hernan Guillermo
Martinez, Gustavo Adolfo
Civello, Pedro Marcos
author2_role author
author
author
dc.subject.none.fl_str_mv CELL WALL
FRAGARIA
PECTIN
POLYGALACTURONASE
SOFTENING
topic CELL WALL
FRAGARIA
PECTIN
POLYGALACTURONASE
SOFTENING
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fleshy fruits soften during ripening mainly as a consequence of the catabolism of cell wall components. In strawberry (Fragaria × ananassa Duch), the depolymerization and solubilization of pectins increase during ripening and contribute to fruit softening. In the present paper, we report the cloning and expression analysis of two polygalacturonase (PG) putative cDNAs: FaPG1 and T-PG. The former seems to be the same sequence of previously reported PG in strawberry, while T-PG cDNA has a deletion of 85 bp that cause a frame shift and would produce an inactive protein. Measurement of total PG activity and expression of FaPG1 and T-PG were performed in strawberry cultivars with contrasting softening rates. The softest cultivar (Toyonaka) showed the higher total PG activity in all ripening stages analyzed. The analysis by RT-PCR revealed that both genes express in the three cultivars, though the expression pattern was different. In the firmer cultivars (Selva and Camarosa) the expression of T-PG was considerably higher than the expression of FaPG1, while the opposite occurred in the softest cultivar (Toyonaka). Therefore, the higher PG activity detected in Toyonaka correlates with the enhanced expression of FaPG1 gene, while the low PG activity found in the firm cultivars correlates with a higher expression of T-PG, a gene that could encode a truncated protein without PG activity. The influence of auxins on both the expression of PG genes and the total PG activity during strawberry fruit ripening was also analyzed.
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description Fleshy fruits soften during ripening mainly as a consequence of the catabolism of cell wall components. In strawberry (Fragaria × ananassa Duch), the depolymerization and solubilization of pectins increase during ripening and contribute to fruit softening. In the present paper, we report the cloning and expression analysis of two polygalacturonase (PG) putative cDNAs: FaPG1 and T-PG. The former seems to be the same sequence of previously reported PG in strawberry, while T-PG cDNA has a deletion of 85 bp that cause a frame shift and would produce an inactive protein. Measurement of total PG activity and expression of FaPG1 and T-PG were performed in strawberry cultivars with contrasting softening rates. The softest cultivar (Toyonaka) showed the higher total PG activity in all ripening stages analyzed. The analysis by RT-PCR revealed that both genes express in the three cultivars, though the expression pattern was different. In the firmer cultivars (Selva and Camarosa) the expression of T-PG was considerably higher than the expression of FaPG1, while the opposite occurred in the softest cultivar (Toyonaka). Therefore, the higher PG activity detected in Toyonaka correlates with the enhanced expression of FaPG1 gene, while the low PG activity found in the firm cultivars correlates with a higher expression of T-PG, a gene that could encode a truncated protein without PG activity. The influence of auxins on both the expression of PG genes and the total PG activity during strawberry fruit ripening was also analyzed.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155127
Villarreal, Natalia Marina; Rosli, Hernan Guillermo; Martinez, Gustavo Adolfo; Civello, Pedro Marcos; Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness; Elsevier Science; Postharvest Biology and Technology; 47; 2; 2-2008; 141-150
0925-5214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155127
identifier_str_mv Villarreal, Natalia Marina; Rosli, Hernan Guillermo; Martinez, Gustavo Adolfo; Civello, Pedro Marcos; Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness; Elsevier Science; Postharvest Biology and Technology; 47; 2; 2-2008; 141-150
0925-5214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2007.06.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925521407002177?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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