Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )

Autores
Navarro Ródenas, Alfonso; Xu, Hao; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Zwiazek, Janusz J.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of ectomycorrhizal associations is crucial for growth of many forest trees. However, the signals that are exchanged between the fungus and the host plant during the colonization process are still poorly understood. In this study, we have identified the relationship between expression patterns of Laccaria bicolor aquaporin LbAQP1 and the development of ectomycorrhizal structures in trembling aspen (Populus tremuloides) seedlings. The peak expression of LbAQP1 was 700‐fold higher in the hyphae within the root than in the free‐living mycelium after 24 h of direct interaction with the roots. Moreover, in LbAQP1 knock‐down strains, a non‐mycorrhizal phenotype was developed without the Hartig net and the expression of the mycorrhizal effector protein MiSSP7 quickly declined after an initial peak on day 5 of interaction of the fungal hyphae with the roots. The increase in the expression of LbAQP1 required a direct contact of the fungus with the root and it modulated the expression of MiSSP7. We have also determined that LbAQP1 facilitated NO, H2O2 and CO2 transport when heterologously expressed in yeast. The report demonstrates that the L. bicolor aquaporin LbAQP1 acts as a molecular signalling channel, which is fundamental for the development of Hartig net in root tips of P. tremuloides.
Fil: Navarro Ródenas, Alfonso. University of Alberta; Canadá
Fil: Xu, Hao. University of Alberta; Canadá
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zwiazek, Janusz J.. University of Alberta; Canadá
Materia
Ectomycorrhiza
Laccaria
Aspen
Aquaporins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41573
Acceder
id CONICETDig_8c3be63071648652ad2d6364d3661205
oai_identifier_str oai:ri.conicet.gov.ar:11336/41573
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )Navarro Ródenas, AlfonsoXu, HaoKemppainen, Minna JohannaPardo, Alejandro GuillermoZwiazek, Janusz J.EctomycorrhizaLaccariaAspenAquaporinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The development of ectomycorrhizal associations is crucial for growth of many forest trees. However, the signals that are exchanged between the fungus and the host plant during the colonization process are still poorly understood. In this study, we have identified the relationship between expression patterns of Laccaria bicolor aquaporin LbAQP1 and the development of ectomycorrhizal structures in trembling aspen (Populus tremuloides) seedlings. The peak expression of LbAQP1 was 700‐fold higher in the hyphae within the root than in the free‐living mycelium after 24 h of direct interaction with the roots. Moreover, in LbAQP1 knock‐down strains, a non‐mycorrhizal phenotype was developed without the Hartig net and the expression of the mycorrhizal effector protein MiSSP7 quickly declined after an initial peak on day 5 of interaction of the fungal hyphae with the roots. The increase in the expression of LbAQP1 required a direct contact of the fungus with the root and it modulated the expression of MiSSP7. We have also determined that LbAQP1 facilitated NO, H2O2 and CO2 transport when heterologously expressed in yeast. The report demonstrates that the L. bicolor aquaporin LbAQP1 acts as a molecular signalling channel, which is fundamental for the development of Hartig net in root tips of P. tremuloides.Fil: Navarro Ródenas, Alfonso. University of Alberta; CanadáFil: Xu, Hao. University of Alberta; CanadáFil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zwiazek, Janusz J.. University of Alberta; CanadáWiley Blackwell Publishing, Inc2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41573Navarro Ródenas, Alfonso; Xu, Hao; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Zwiazek, Janusz J.; Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides ); Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 38; 11; 11-2015; 2475-24860140-7791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/pce.12552info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12552info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:33Zoai:ri.conicet.gov.ar:11336/41573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:33.296CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
title Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
spellingShingle Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
Navarro Ródenas, Alfonso
Ectomycorrhiza
Laccaria
Aspen
Aquaporins
title_short Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
title_full Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
title_fullStr Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
title_full_unstemmed Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
title_sort Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides )
dc.creator.none.fl_str_mv Navarro Ródenas, Alfonso
Xu, Hao
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Zwiazek, Janusz J.
author Navarro Ródenas, Alfonso
author_facet Navarro Ródenas, Alfonso
Xu, Hao
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Zwiazek, Janusz J.
author_role author
author2 Xu, Hao
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Zwiazek, Janusz J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ectomycorrhiza
Laccaria
Aspen
Aquaporins
topic Ectomycorrhiza
Laccaria
Aspen
Aquaporins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of ectomycorrhizal associations is crucial for growth of many forest trees. However, the signals that are exchanged between the fungus and the host plant during the colonization process are still poorly understood. In this study, we have identified the relationship between expression patterns of Laccaria bicolor aquaporin LbAQP1 and the development of ectomycorrhizal structures in trembling aspen (Populus tremuloides) seedlings. The peak expression of LbAQP1 was 700‐fold higher in the hyphae within the root than in the free‐living mycelium after 24 h of direct interaction with the roots. Moreover, in LbAQP1 knock‐down strains, a non‐mycorrhizal phenotype was developed without the Hartig net and the expression of the mycorrhizal effector protein MiSSP7 quickly declined after an initial peak on day 5 of interaction of the fungal hyphae with the roots. The increase in the expression of LbAQP1 required a direct contact of the fungus with the root and it modulated the expression of MiSSP7. We have also determined that LbAQP1 facilitated NO, H2O2 and CO2 transport when heterologously expressed in yeast. The report demonstrates that the L. bicolor aquaporin LbAQP1 acts as a molecular signalling channel, which is fundamental for the development of Hartig net in root tips of P. tremuloides.
Fil: Navarro Ródenas, Alfonso. University of Alberta; Canadá
Fil: Xu, Hao. University of Alberta; Canadá
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zwiazek, Janusz J.. University of Alberta; Canadá
description The development of ectomycorrhizal associations is crucial for growth of many forest trees. However, the signals that are exchanged between the fungus and the host plant during the colonization process are still poorly understood. In this study, we have identified the relationship between expression patterns of Laccaria bicolor aquaporin LbAQP1 and the development of ectomycorrhizal structures in trembling aspen (Populus tremuloides) seedlings. The peak expression of LbAQP1 was 700‐fold higher in the hyphae within the root than in the free‐living mycelium after 24 h of direct interaction with the roots. Moreover, in LbAQP1 knock‐down strains, a non‐mycorrhizal phenotype was developed without the Hartig net and the expression of the mycorrhizal effector protein MiSSP7 quickly declined after an initial peak on day 5 of interaction of the fungal hyphae with the roots. The increase in the expression of LbAQP1 required a direct contact of the fungus with the root and it modulated the expression of MiSSP7. We have also determined that LbAQP1 facilitated NO, H2O2 and CO2 transport when heterologously expressed in yeast. The report demonstrates that the L. bicolor aquaporin LbAQP1 acts as a molecular signalling channel, which is fundamental for the development of Hartig net in root tips of P. tremuloides.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41573
Navarro Ródenas, Alfonso; Xu, Hao; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Zwiazek, Janusz J.; Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides ); Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 38; 11; 11-2015; 2475-2486
0140-7791
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41573
identifier_str_mv Navarro Ródenas, Alfonso; Xu, Hao; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Zwiazek, Janusz J.; Laccaria bicolor aquaporin LbAQP1 is required for Hartig net development in trembling aspen ( Populus tremuloides ); Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 38; 11; 11-2015; 2475-2486
0140-7791
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/pce.12552
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12552
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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