Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study
- Autores
- Arias, Juan Marcelo; Cobos Picot, Rafael Alejandro; Tuttolomondo, María Eugenia; Ben Altabef, Aida; Díaz, Sonia Beatriz
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The N-acetylcysteine (NAC) is a commonly used mucolytic and antioxidant agent. The knowledgeof interactions of the NAC with cell membranes is important to understand its mechanism ofpharmacological action at the molecular level. To gain a deeper insight into analyzing N-acetylcysteineinteractions with multilamellar liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) inhydrated states at different pH, we performed experimental studies by differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The behavior of theasymmetric stretching band of the PO2 group was studied by FTIR following the changes that occur inthe shape of this band in the pretransition and transition stages from the gel state to the crystallineliquid state. The values of the pretransition temperature (Tp) for different molar ratios of the complex atthe two pH values studied were coincident between the measurements by FTIR and DSC, with theresults reaffirming the importance of the PO2 group as a sensor not only in the state of hydration butalso in the formation of new intermolecular bonds, which lead to the formation of different domains.The vibrational behavior of the groups of the polar head of the lipid revealed the role of the lipid as anoxidant on the thiol site of the NAC. The results of these studies provide information on membraneintegrity and its physicochemical properties. In this aspect, this work has novelty and practicality inpharmacology and analytical chemistry
Fil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Cobos Picot, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina - Materia
-
LIPIDOMIC
INTERACTIONS
DPPC
CYSTEINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140832
Ver los metadatos del registro completo
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Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical studyArias, Juan MarceloCobos Picot, Rafael AlejandroTuttolomondo, María EugeniaBen Altabef, AidaDíaz, Sonia BeatrizLIPIDOMICINTERACTIONSDPPCCYSTEINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The N-acetylcysteine (NAC) is a commonly used mucolytic and antioxidant agent. The knowledgeof interactions of the NAC with cell membranes is important to understand its mechanism ofpharmacological action at the molecular level. To gain a deeper insight into analyzing N-acetylcysteineinteractions with multilamellar liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) inhydrated states at different pH, we performed experimental studies by differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The behavior of theasymmetric stretching band of the PO2 group was studied by FTIR following the changes that occur inthe shape of this band in the pretransition and transition stages from the gel state to the crystallineliquid state. The values of the pretransition temperature (Tp) for different molar ratios of the complex atthe two pH values studied were coincident between the measurements by FTIR and DSC, with theresults reaffirming the importance of the PO2 group as a sensor not only in the state of hydration butalso in the formation of new intermolecular bonds, which lead to the formation of different domains.The vibrational behavior of the groups of the polar head of the lipid revealed the role of the lipid as anoxidant on the thiol site of the NAC. The results of these studies provide information on membraneintegrity and its physicochemical properties. In this aspect, this work has novelty and practicality inpharmacology and analytical chemistryFil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Cobos Picot, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaRoyal Society of Chemistry2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140832Arias, Juan Marcelo; Cobos Picot, Rafael Alejandro; Tuttolomondo, María Eugenia; Ben Altabef, Aida; Díaz, Sonia Beatriz; Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study; Royal Society of Chemistry; New Journal of Chemistry; 44; 35; 8-2020; 14837-148481144-0546CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2020/NJ/C9NJ06167Cinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C9NJ06167Cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:38Zoai:ri.conicet.gov.ar:11336/140832instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:38.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| title |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| spellingShingle |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study Arias, Juan Marcelo LIPIDOMIC INTERACTIONS DPPC CYSTEINE |
| title_short |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| title_full |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| title_fullStr |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| title_full_unstemmed |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| title_sort |
Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study |
| dc.creator.none.fl_str_mv |
Arias, Juan Marcelo Cobos Picot, Rafael Alejandro Tuttolomondo, María Eugenia Ben Altabef, Aida Díaz, Sonia Beatriz |
| author |
Arias, Juan Marcelo |
| author_facet |
Arias, Juan Marcelo Cobos Picot, Rafael Alejandro Tuttolomondo, María Eugenia Ben Altabef, Aida Díaz, Sonia Beatriz |
| author_role |
author |
| author2 |
Cobos Picot, Rafael Alejandro Tuttolomondo, María Eugenia Ben Altabef, Aida Díaz, Sonia Beatriz |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
LIPIDOMIC INTERACTIONS DPPC CYSTEINE |
| topic |
LIPIDOMIC INTERACTIONS DPPC CYSTEINE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The N-acetylcysteine (NAC) is a commonly used mucolytic and antioxidant agent. The knowledgeof interactions of the NAC with cell membranes is important to understand its mechanism ofpharmacological action at the molecular level. To gain a deeper insight into analyzing N-acetylcysteineinteractions with multilamellar liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) inhydrated states at different pH, we performed experimental studies by differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The behavior of theasymmetric stretching band of the PO2 group was studied by FTIR following the changes that occur inthe shape of this band in the pretransition and transition stages from the gel state to the crystallineliquid state. The values of the pretransition temperature (Tp) for different molar ratios of the complex atthe two pH values studied were coincident between the measurements by FTIR and DSC, with theresults reaffirming the importance of the PO2 group as a sensor not only in the state of hydration butalso in the formation of new intermolecular bonds, which lead to the formation of different domains.The vibrational behavior of the groups of the polar head of the lipid revealed the role of the lipid as anoxidant on the thiol site of the NAC. The results of these studies provide information on membraneintegrity and its physicochemical properties. In this aspect, this work has novelty and practicality inpharmacology and analytical chemistry Fil: Arias, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Cobos Picot, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Tuttolomondo, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Díaz, Sonia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina |
| description |
The N-acetylcysteine (NAC) is a commonly used mucolytic and antioxidant agent. The knowledgeof interactions of the NAC with cell membranes is important to understand its mechanism ofpharmacological action at the molecular level. To gain a deeper insight into analyzing N-acetylcysteineinteractions with multilamellar liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) inhydrated states at different pH, we performed experimental studies by differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The behavior of theasymmetric stretching band of the PO2 group was studied by FTIR following the changes that occur inthe shape of this band in the pretransition and transition stages from the gel state to the crystallineliquid state. The values of the pretransition temperature (Tp) for different molar ratios of the complex atthe two pH values studied were coincident between the measurements by FTIR and DSC, with theresults reaffirming the importance of the PO2 group as a sensor not only in the state of hydration butalso in the formation of new intermolecular bonds, which lead to the formation of different domains.The vibrational behavior of the groups of the polar head of the lipid revealed the role of the lipid as anoxidant on the thiol site of the NAC. The results of these studies provide information on membraneintegrity and its physicochemical properties. In this aspect, this work has novelty and practicality inpharmacology and analytical chemistry |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-08 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/140832 Arias, Juan Marcelo; Cobos Picot, Rafael Alejandro; Tuttolomondo, María Eugenia; Ben Altabef, Aida; Díaz, Sonia Beatriz; Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study; Royal Society of Chemistry; New Journal of Chemistry; 44; 35; 8-2020; 14837-14848 1144-0546 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/140832 |
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Arias, Juan Marcelo; Cobos Picot, Rafael Alejandro; Tuttolomondo, María Eugenia; Ben Altabef, Aida; Díaz, Sonia Beatriz; Interaction of N -acetylcysteine with DPPC liposomes at different pH: a physicochemical study; Royal Society of Chemistry; New Journal of Chemistry; 44; 35; 8-2020; 14837-14848 1144-0546 CONICET Digital CONICET |
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eng |
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Royal Society of Chemistry |
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Royal Society of Chemistry |
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