Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment

Autores
Palomeque, Miriam Edid; Ortiz, Patricia Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two automated methods for the determination of humic acids (HAs) in natural water samples are presented. Flow injection systems were developed based on the formation of a complex between humic acids and toluidine blue dye. When the complex is formed, the dye absorbance signal decreases at selected operating conditions. Method A, without sample pretreatment, presents a linear calibration curve over HA concentration range 1.14−35.0 μg mL−1 , with a limit of detection (LOD) of 0.4 μg mL−1 (3 Sy/x) and a sample throughput of 80 h−1 . To determine lower HA concentration levels, a preceding separation/preconcentration step was included (method B). This method has a linear range of 1.5−20.0 μg mL−1 and a sample throughput of >8 h−1 and could detect humic substances at a concentration of 0.05 μg mL−1 after a 4-min retention time. The RSDs for methods A and B calculated using the slopes of seven independent calibration graphs obtained on different days and with different conditions (standard solution, reagent solution, etc.) were 4.5% and 4.2% respectively. Both methods were satisfactorily applied to natural water samples, with recovery percentages in the 95−107% range, and compare very well with other methods.
Fil: Palomeque, Miriam Edid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Humic Acids
Flow Injection
Water
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25837
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatmentPalomeque, Miriam EdidOrtiz, Patricia InesHumic AcidsFlow InjectionWaterhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Two automated methods for the determination of humic acids (HAs) in natural water samples are presented. Flow injection systems were developed based on the formation of a complex between humic acids and toluidine blue dye. When the complex is formed, the dye absorbance signal decreases at selected operating conditions. Method A, without sample pretreatment, presents a linear calibration curve over HA concentration range 1.14−35.0 μg mL−1 , with a limit of detection (LOD) of 0.4 μg mL−1 (3 Sy/x) and a sample throughput of 80 h−1 . To determine lower HA concentration levels, a preceding separation/preconcentration step was included (method B). This method has a linear range of 1.5−20.0 μg mL−1 and a sample throughput of >8 h−1 and could detect humic substances at a concentration of 0.05 μg mL−1 after a 4-min retention time. The RSDs for methods A and B calculated using the slopes of seven independent calibration graphs obtained on different days and with different conditions (standard solution, reagent solution, etc.) were 4.5% and 4.2% respectively. Both methods were satisfactorily applied to natural water samples, with recovery percentages in the 95−107% range, and compare very well with other methods.Fil: Palomeque, Miriam Edid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaAmerican Chemical Society2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25837Palomeque, Miriam Edid; Ortiz, Patricia Ines; Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment; American Chemical Society; Industrial & Engineering Chemical Research; 52; 36; 8-2013; 12717-127220888-5885CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ie400980uinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ie400980uinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:02Zoai:ri.conicet.gov.ar:11336/25837instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:02.879CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
title Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
spellingShingle Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
Palomeque, Miriam Edid
Humic Acids
Flow Injection
Water
title_short Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
title_full Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
title_fullStr Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
title_full_unstemmed Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
title_sort Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment
dc.creator.none.fl_str_mv Palomeque, Miriam Edid
Ortiz, Patricia Ines
author Palomeque, Miriam Edid
author_facet Palomeque, Miriam Edid
Ortiz, Patricia Ines
author_role author
author2 Ortiz, Patricia Ines
author2_role author
dc.subject.none.fl_str_mv Humic Acids
Flow Injection
Water
topic Humic Acids
Flow Injection
Water
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two automated methods for the determination of humic acids (HAs) in natural water samples are presented. Flow injection systems were developed based on the formation of a complex between humic acids and toluidine blue dye. When the complex is formed, the dye absorbance signal decreases at selected operating conditions. Method A, without sample pretreatment, presents a linear calibration curve over HA concentration range 1.14−35.0 μg mL−1 , with a limit of detection (LOD) of 0.4 μg mL−1 (3 Sy/x) and a sample throughput of 80 h−1 . To determine lower HA concentration levels, a preceding separation/preconcentration step was included (method B). This method has a linear range of 1.5−20.0 μg mL−1 and a sample throughput of >8 h−1 and could detect humic substances at a concentration of 0.05 μg mL−1 after a 4-min retention time. The RSDs for methods A and B calculated using the slopes of seven independent calibration graphs obtained on different days and with different conditions (standard solution, reagent solution, etc.) were 4.5% and 4.2% respectively. Both methods were satisfactorily applied to natural water samples, with recovery percentages in the 95−107% range, and compare very well with other methods.
Fil: Palomeque, Miriam Edid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Two automated methods for the determination of humic acids (HAs) in natural water samples are presented. Flow injection systems were developed based on the formation of a complex between humic acids and toluidine blue dye. When the complex is formed, the dye absorbance signal decreases at selected operating conditions. Method A, without sample pretreatment, presents a linear calibration curve over HA concentration range 1.14−35.0 μg mL−1 , with a limit of detection (LOD) of 0.4 μg mL−1 (3 Sy/x) and a sample throughput of 80 h−1 . To determine lower HA concentration levels, a preceding separation/preconcentration step was included (method B). This method has a linear range of 1.5−20.0 μg mL−1 and a sample throughput of >8 h−1 and could detect humic substances at a concentration of 0.05 μg mL−1 after a 4-min retention time. The RSDs for methods A and B calculated using the slopes of seven independent calibration graphs obtained on different days and with different conditions (standard solution, reagent solution, etc.) were 4.5% and 4.2% respectively. Both methods were satisfactorily applied to natural water samples, with recovery percentages in the 95−107% range, and compare very well with other methods.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25837
Palomeque, Miriam Edid; Ortiz, Patricia Ines; Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment; American Chemical Society; Industrial & Engineering Chemical Research; 52; 36; 8-2013; 12717-12722
0888-5885
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25837
identifier_str_mv Palomeque, Miriam Edid; Ortiz, Patricia Ines; Water-soluble humic acid quantification using a flow-injection system with and without sample pretreatment; American Chemical Society; Industrial & Engineering Chemical Research; 52; 36; 8-2013; 12717-12722
0888-5885
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ie400980u
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ie400980u
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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