Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain

Autores
Cabral, Maria Eugenia; Joya, Constanza María; Figueroa, L. I. C.; Fariña, Julia Ines
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Statins can inhibit the de novo cholesterol biosynthesis at the ratelimiting step of HMG-CoA reductase catalysis. For this reason,statins are clinically used as effective drugs forhypercholesterolemia treatment. Objective. This work was aimed atoptimizing and simplifying medium composition and fermentationconditions at shake-flask scale. In a second stage, these results weresubsequently applied for the massive lovastatin production by SolidSubstrate Fermentation (SSF). Methodology. Aspergillus terreusMEC was cultured by submerged fermentation (SF) in lactose-yeastextract medium, in shake flasks at 250 rpm and 25ºC during 14 days.Optimization included different C- and N- sources and the decreaseof salts and trace elements concentration. Additionally, theinfluence of soy flour incorporation and the replacement of lactoseby milk whey powder were evaluated. Different solid supports(sugarcane bagasse, wheat straw, soy flour) for SSF were assayed.Extracted lovastatin was analyzed by RP-HPLC with a Diode ArrayDetector. Results and conclusions. A lovastatin production of 63mg/L could be reached with optimized liquid medium. Productionwas significantly increased, up to ~1000 mg/L, by SSF includingcheap and readily available substrates such as whey milk adsorbedon textured soy flour, highlighting the relevance of fermentationstrategies for secondary metabolite production.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
LOVASTATIN
SOLID-STATE
ASPERGILLUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194026
Acceder
id CONICETDig_2ddf19857c9dc03fb5b0798fcb3fa612
oai_identifier_str oai:ri.conicet.gov.ar:11336/194026
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strainCabral, Maria EugeniaJoya, Constanza MaríaFigueroa, L. I. C.Fariña, Julia InesLOVASTATINSOLID-STATEASPERGILLUShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Statins can inhibit the de novo cholesterol biosynthesis at the ratelimiting step of HMG-CoA reductase catalysis. For this reason,statins are clinically used as effective drugs forhypercholesterolemia treatment. Objective. This work was aimed atoptimizing and simplifying medium composition and fermentationconditions at shake-flask scale. In a second stage, these results weresubsequently applied for the massive lovastatin production by SolidSubstrate Fermentation (SSF). Methodology. Aspergillus terreusMEC was cultured by submerged fermentation (SF) in lactose-yeastextract medium, in shake flasks at 250 rpm and 25ºC during 14 days.Optimization included different C- and N- sources and the decreaseof salts and trace elements concentration. Additionally, theinfluence of soy flour incorporation and the replacement of lactoseby milk whey powder were evaluated. Different solid supports(sugarcane bagasse, wheat straw, soy flour) for SSF were assayed.Extracted lovastatin was analyzed by RP-HPLC with a Diode ArrayDetector. Results and conclusions. A lovastatin production of 63mg/L could be reached with optimized liquid medium. Productionwas significantly increased, up to ~1000 mg/L, by SSF includingcheap and readily available substrates such as whey milk adsorbedon textured soy flour, highlighting the relevance of fermentationstrategies for secondary metabolite production.Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194026Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-21667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:14Zoai:ri.conicet.gov.ar:11336/194026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:15.219CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
title Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
spellingShingle Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
Cabral, Maria Eugenia
LOVASTATIN
SOLID-STATE
ASPERGILLUS
title_short Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
title_full Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
title_fullStr Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
title_full_unstemmed Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
title_sort Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain
dc.creator.none.fl_str_mv Cabral, Maria Eugenia
Joya, Constanza María
Figueroa, L. I. C.
Fariña, Julia Ines
author Cabral, Maria Eugenia
author_facet Cabral, Maria Eugenia
Joya, Constanza María
Figueroa, L. I. C.
Fariña, Julia Ines
author_role author
author2 Joya, Constanza María
Figueroa, L. I. C.
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv LOVASTATIN
SOLID-STATE
ASPERGILLUS
topic LOVASTATIN
SOLID-STATE
ASPERGILLUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Statins can inhibit the de novo cholesterol biosynthesis at the ratelimiting step of HMG-CoA reductase catalysis. For this reason,statins are clinically used as effective drugs forhypercholesterolemia treatment. Objective. This work was aimed atoptimizing and simplifying medium composition and fermentationconditions at shake-flask scale. In a second stage, these results weresubsequently applied for the massive lovastatin production by SolidSubstrate Fermentation (SSF). Methodology. Aspergillus terreusMEC was cultured by submerged fermentation (SF) in lactose-yeastextract medium, in shake flasks at 250 rpm and 25ºC during 14 days.Optimization included different C- and N- sources and the decreaseof salts and trace elements concentration. Additionally, theinfluence of soy flour incorporation and the replacement of lactoseby milk whey powder were evaluated. Different solid supports(sugarcane bagasse, wheat straw, soy flour) for SSF were assayed.Extracted lovastatin was analyzed by RP-HPLC with a Diode ArrayDetector. Results and conclusions. A lovastatin production of 63mg/L could be reached with optimized liquid medium. Productionwas significantly increased, up to ~1000 mg/L, by SSF includingcheap and readily available substrates such as whey milk adsorbedon textured soy flour, highlighting the relevance of fermentationstrategies for secondary metabolite production.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Statins can inhibit the de novo cholesterol biosynthesis at the ratelimiting step of HMG-CoA reductase catalysis. For this reason,statins are clinically used as effective drugs forhypercholesterolemia treatment. Objective. This work was aimed atoptimizing and simplifying medium composition and fermentationconditions at shake-flask scale. In a second stage, these results weresubsequently applied for the massive lovastatin production by SolidSubstrate Fermentation (SSF). Methodology. Aspergillus terreusMEC was cultured by submerged fermentation (SF) in lactose-yeastextract medium, in shake flasks at 250 rpm and 25ºC during 14 days.Optimization included different C- and N- sources and the decreaseof salts and trace elements concentration. Additionally, theinfluence of soy flour incorporation and the replacement of lactoseby milk whey powder were evaluated. Different solid supports(sugarcane bagasse, wheat straw, soy flour) for SSF were assayed.Extracted lovastatin was analyzed by RP-HPLC with a Diode ArrayDetector. Results and conclusions. A lovastatin production of 63mg/L could be reached with optimized liquid medium. Productionwas significantly increased, up to ~1000 mg/L, by SSF includingcheap and readily available substrates such as whey milk adsorbedon textured soy flour, highlighting the relevance of fermentationstrategies for secondary metabolite production.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194026
Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-2
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194026
identifier_str_mv Optimized lovastatin production by solid-state fermentation with aspergillus terreus wildtype strain; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-2
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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