The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells

Autores
Charó, Nancy Lorena; Jerez, Horacio Emanuel; Tatti, Silvio Alejandro; Romero, Eder Lilia; Schattner, Mirta Ana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Archaebacterias are considered a unique source of novel biomaterials of interest for nanomedicine. In this perspective, the effects of nanoarchaeosomes (ARC), which are nanovesi-cles prepared from polar lipids extracted from the extreme halophilic Halorubrum tebenquinchense, on human umbilical vein endothelial cells (HUVEC) were investigated in physiological and under inflammatory static conditions. Upon incubation, ARC (170 nm mean size, −41 mV ζ) did not affect viability, cell proliferation, and expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin under basal conditions, but reduced expression of both molecules and secretion of IL-6 induced by lypopolysaccharide (LPS), Pam3CSK4 or Escherichia coli. Such effects were not observed with TNF-α or IL-1β stimulation. Interestingly, ARC significantly decreased basal levels of von Willebrand factor (vWF) and levels induced by all stimuli. None of these parameters was altered by liposomes of hydrogenated phosphatidylcholine and cholesterol of comparable size and concentra-tion. Only ARC were endocytosed by HUVEC and reduced mRNA expression of ICAM-1 and vWF via NF-kB and ERK1/2 in LPS-stimulated cells. This is the first report of the anti-inflammatory effect of ARC on endothelial cells and our data suggest that its future use in vascular disease may hopefully be of particular interest.
Fil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Jerez, Horacio Emanuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tatti, Silvio Alejandro. No especifíca;
Fil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
ENDOTHELIUM
ICAM-1
INFLAMMATION
NANOARCHAEOSOMES
NANOVESICLES
VON WILLEBRAND FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/187102
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/187102
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial CellsCharó, Nancy LorenaJerez, Horacio EmanuelTatti, Silvio AlejandroRomero, Eder LiliaSchattner, Mirta AnaENDOTHELIUMICAM-1INFLAMMATIONNANOARCHAEOSOMESNANOVESICLESVON WILLEBRAND FACTORhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Archaebacterias are considered a unique source of novel biomaterials of interest for nanomedicine. In this perspective, the effects of nanoarchaeosomes (ARC), which are nanovesi-cles prepared from polar lipids extracted from the extreme halophilic Halorubrum tebenquinchense, on human umbilical vein endothelial cells (HUVEC) were investigated in physiological and under inflammatory static conditions. Upon incubation, ARC (170 nm mean size, −41 mV ζ) did not affect viability, cell proliferation, and expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin under basal conditions, but reduced expression of both molecules and secretion of IL-6 induced by lypopolysaccharide (LPS), Pam3CSK4 or Escherichia coli. Such effects were not observed with TNF-α or IL-1β stimulation. Interestingly, ARC significantly decreased basal levels of von Willebrand factor (vWF) and levels induced by all stimuli. None of these parameters was altered by liposomes of hydrogenated phosphatidylcholine and cholesterol of comparable size and concentra-tion. Only ARC were endocytosed by HUVEC and reduced mRNA expression of ICAM-1 and vWF via NF-kB and ERK1/2 in LPS-stimulated cells. This is the first report of the anti-inflammatory effect of ARC on endothelial cells and our data suggest that its future use in vascular disease may hopefully be of particular interest.Fil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Jerez, Horacio Emanuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tatti, Silvio Alejandro. No especifíca;Fil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; ArgentinaFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaMDPI2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/187102Charó, Nancy Lorena; Jerez, Horacio Emanuel; Tatti, Silvio Alejandro; Romero, Eder Lilia; Schattner, Mirta Ana; The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells; MDPI; Pharmaceutics; 14; 4; 4-2022; 1-191999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics14040736info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/14/4/736info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:12Zoai:ri.conicet.gov.ar:11336/187102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:12.909CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
title The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
spellingShingle The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
Charó, Nancy Lorena
ENDOTHELIUM
ICAM-1
INFLAMMATION
NANOARCHAEOSOMES
NANOVESICLES
VON WILLEBRAND FACTOR
title_short The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
title_full The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
title_fullStr The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
title_full_unstemmed The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
title_sort The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells
dc.creator.none.fl_str_mv Charó, Nancy Lorena
Jerez, Horacio Emanuel
Tatti, Silvio Alejandro
Romero, Eder Lilia
Schattner, Mirta Ana
author Charó, Nancy Lorena
author_facet Charó, Nancy Lorena
Jerez, Horacio Emanuel
Tatti, Silvio Alejandro
Romero, Eder Lilia
Schattner, Mirta Ana
author_role author
author2 Jerez, Horacio Emanuel
Tatti, Silvio Alejandro
Romero, Eder Lilia
Schattner, Mirta Ana
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENDOTHELIUM
ICAM-1
INFLAMMATION
NANOARCHAEOSOMES
NANOVESICLES
VON WILLEBRAND FACTOR
topic ENDOTHELIUM
ICAM-1
INFLAMMATION
NANOARCHAEOSOMES
NANOVESICLES
VON WILLEBRAND FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Archaebacterias are considered a unique source of novel biomaterials of interest for nanomedicine. In this perspective, the effects of nanoarchaeosomes (ARC), which are nanovesi-cles prepared from polar lipids extracted from the extreme halophilic Halorubrum tebenquinchense, on human umbilical vein endothelial cells (HUVEC) were investigated in physiological and under inflammatory static conditions. Upon incubation, ARC (170 nm mean size, −41 mV ζ) did not affect viability, cell proliferation, and expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin under basal conditions, but reduced expression of both molecules and secretion of IL-6 induced by lypopolysaccharide (LPS), Pam3CSK4 or Escherichia coli. Such effects were not observed with TNF-α or IL-1β stimulation. Interestingly, ARC significantly decreased basal levels of von Willebrand factor (vWF) and levels induced by all stimuli. None of these parameters was altered by liposomes of hydrogenated phosphatidylcholine and cholesterol of comparable size and concentra-tion. Only ARC were endocytosed by HUVEC and reduced mRNA expression of ICAM-1 and vWF via NF-kB and ERK1/2 in LPS-stimulated cells. This is the first report of the anti-inflammatory effect of ARC on endothelial cells and our data suggest that its future use in vascular disease may hopefully be of particular interest.
Fil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Jerez, Horacio Emanuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tatti, Silvio Alejandro. No especifíca;
Fil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description Archaebacterias are considered a unique source of novel biomaterials of interest for nanomedicine. In this perspective, the effects of nanoarchaeosomes (ARC), which are nanovesi-cles prepared from polar lipids extracted from the extreme halophilic Halorubrum tebenquinchense, on human umbilical vein endothelial cells (HUVEC) were investigated in physiological and under inflammatory static conditions. Upon incubation, ARC (170 nm mean size, −41 mV ζ) did not affect viability, cell proliferation, and expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin under basal conditions, but reduced expression of both molecules and secretion of IL-6 induced by lypopolysaccharide (LPS), Pam3CSK4 or Escherichia coli. Such effects were not observed with TNF-α or IL-1β stimulation. Interestingly, ARC significantly decreased basal levels of von Willebrand factor (vWF) and levels induced by all stimuli. None of these parameters was altered by liposomes of hydrogenated phosphatidylcholine and cholesterol of comparable size and concentra-tion. Only ARC were endocytosed by HUVEC and reduced mRNA expression of ICAM-1 and vWF via NF-kB and ERK1/2 in LPS-stimulated cells. This is the first report of the anti-inflammatory effect of ARC on endothelial cells and our data suggest that its future use in vascular disease may hopefully be of particular interest.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/187102
Charó, Nancy Lorena; Jerez, Horacio Emanuel; Tatti, Silvio Alejandro; Romero, Eder Lilia; Schattner, Mirta Ana; The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells; MDPI; Pharmaceutics; 14; 4; 4-2022; 1-19
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/187102
identifier_str_mv Charó, Nancy Lorena; Jerez, Horacio Emanuel; Tatti, Silvio Alejandro; Romero, Eder Lilia; Schattner, Mirta Ana; The Anti-Inflammatory Effect of Nanoarchaeosomes on Human Endothelial Cells; MDPI; Pharmaceutics; 14; 4; 4-2022; 1-19
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics14040736
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/14/4/736
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981398475964416
score 12.48226

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