Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light

Autores
Barchuk, Mónica Lucrecia; Díaz, Gabriela Verónica; Coll, Pedro Agustín Francisco; Velázquez, Juan Ernesto; Fonseca, Maria Isabel; Villalba, Laura; Zapata, Pedro Dario
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.
Fil: Barchuk, Mónica Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
TRICHODERMA
XYLANASES
SUGARCANE BAGASSE
LIGHT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/178938
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/178938
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under LightBarchuk, Mónica LucreciaDíaz, Gabriela VerónicaColl, Pedro Agustín FranciscoVelázquez, Juan ErnestoFonseca, Maria IsabelVillalba, LauraZapata, Pedro DarioTRICHODERMAXYLANASESSUGARCANE BAGASSELIGHThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.Fil: Barchuk, Mónica Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaVital Biotech Research & Training Institute2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/178938Barchuk, Mónica Lucrecia; Díaz, Gabriela Verónica; Coll, Pedro Agustín Francisco; Velázquez, Juan Ernesto; Fonseca, Maria Isabel; et al.; Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light; Vital Biotech Research & Training Institute; International Journal of Recent Biotechnology; 4; 2; 5-2016; 25-342322-0392CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:22Zoai:ri.conicet.gov.ar:11336/178938instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:22.845CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
title Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
spellingShingle Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
Barchuk, Mónica Lucrecia
TRICHODERMA
XYLANASES
SUGARCANE BAGASSE
LIGHT
title_short Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
title_full Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
title_fullStr Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
title_full_unstemmed Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
title_sort Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light
dc.creator.none.fl_str_mv Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, Maria Isabel
Villalba, Laura
Zapata, Pedro Dario
author Barchuk, Mónica Lucrecia
author_facet Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, Maria Isabel
Villalba, Laura
Zapata, Pedro Dario
author_role author
author2 Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, Maria Isabel
Villalba, Laura
Zapata, Pedro Dario
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRICHODERMA
XYLANASES
SUGARCANE BAGASSE
LIGHT
topic TRICHODERMA
XYLANASES
SUGARCANE BAGASSE
LIGHT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.
Fil: Barchuk, Mónica Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/178938
Barchuk, Mónica Lucrecia; Díaz, Gabriela Verónica; Coll, Pedro Agustín Francisco; Velázquez, Juan Ernesto; Fonseca, Maria Isabel; et al.; Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light; Vital Biotech Research & Training Institute; International Journal of Recent Biotechnology; 4; 2; 5-2016; 25-34
2322-0392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/178938
identifier_str_mv Barchuk, Mónica Lucrecia; Díaz, Gabriela Verónica; Coll, Pedro Agustín Francisco; Velázquez, Juan Ernesto; Fonseca, Maria Isabel; et al.; Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light; Vital Biotech Research & Training Institute; International Journal of Recent Biotechnology; 4; 2; 5-2016; 25-34
2322-0392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Vital Biotech Research & Training Institute
publisher.none.fl_str_mv Vital Biotech Research & Training Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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