Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light

Autores
Barchuk, Mónica Lucrecia; Díaz, Gabriela Verónica; Coll, Pedro Agustín Francisco; Velázquez, Juan Ernesto; Fonseca, María Isabel; Villalba, Laura Lidia; Zapata, Pedro Darío
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.
Materia
Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Repositorio
Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
Institución
Universidad Nacional de Misiones
OAI Identificador
oai:rid.unam.edu.ar:20.500.12219/4452
Acceder
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oai_identifier_str oai:rid.unam.edu.ar:20.500.12219/4452
network_acronym_str RIDUNaM
repository_id_str
network_name_str Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
spelling Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under lightBarchuk, Mónica LucreciaDíaz, Gabriela VerónicaColl, Pedro Agustín FranciscoVelázquez, Juan ErnestoFonseca, María IsabelVillalba, Laura LidiaZapata, Pedro DaríoTrichodermaXylanasesSugarcane bagasse (SCB)LightFil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.Vital Biotech (India)2016-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf1.614 MBhttps://hdl.handle.net/20.500.12219/4452enginfo:eu-repo/semantics/altIdentifier/urn/https://www.researchgate.net/profile/Gabriela_Diaz40/publication/335099499_Selection_of_Trichoderma_Strain_to_Enhanced_Cellulase-Poor_Xylanase_Production_Using_Sugarcane_Bagasse_as_Sole_Carbon_Source_under_Light/links/5d4ed57da6fdcc370a8a67e4/Selection-of-Trichoderma-Strain-to-Enhanced-Cellulase-Poor-Xylanase-Production-Using-Sugarcane-Bagasse-as-Sole-Carbon-Source-under-Light.pdfinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)instname:Universidad Nacional de Misiones2025-09-29T15:02:31Zoai:rid.unam.edu.ar:20.500.12219/4452instacron:UNAMInstitucionalhttps://rid.unam.edu.ar/Universidad públicahttps://www.unam.edu.ar/https://rid.unam.edu.ar/oai/rsnrdArgentinaopendoar:2025-09-29 15:02:32.163Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misionesfalse
dc.title.none.fl_str_mv Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
spellingShingle Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
Barchuk, Mónica Lucrecia
Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
title_short Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_full Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_fullStr Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_full_unstemmed Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_sort Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
dc.creator.none.fl_str_mv Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
author Barchuk, Mónica Lucrecia
author_facet Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
author_role author
author2 Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
topic Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
dc.description.none.fl_txt_mv Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness.
description Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12219/4452
url https://hdl.handle.net/20.500.12219/4452
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/urn/https://www.researchgate.net/profile/Gabriela_Diaz40/publication/335099499_Selection_of_Trichoderma_Strain_to_Enhanced_Cellulase-Poor_Xylanase_Production_Using_Sugarcane_Bagasse_as_Sole_Carbon_Source_under_Light/links/5d4ed57da6fdcc370a8a67e4/Selection-of-Trichoderma-Strain-to-Enhanced-Cellulase-Poor-Xylanase-Production-Using-Sugarcane-Bagasse-as-Sole-Carbon-Source-under-Light.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
application/pdf
1.614 MB
dc.publisher.none.fl_str_mv Vital Biotech (India)
publisher.none.fl_str_mv Vital Biotech (India)
dc.source.none.fl_str_mv reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
instname:Universidad Nacional de Misiones
reponame_str Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
collection Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
instname_str Universidad Nacional de Misiones
repository.name.fl_str_mv Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misiones
repository.mail.fl_str_mv
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