Independent anti-angiogenic capacities of coagulation factors X and Xa
- Autores
- Lange, Soledad; Gonzalez, Ibeth; Pinto, Mauricio P.; Arce, Maximiliano; Valenzuela, Rodrigo; Aranda, Evelyn; Elliot, Matias; Alvarez, Marjorie; Henriquez, Soledad; Velazquez, Ethel; Orge, Felipe; Oliva, Bárbara; Gonzalez, Pamela; Villalon, Manuel; Cautivo, Kelly M.; Kalergis, Alexis M.; Pereira, Karla; Mendoza, Camila; Saez, Claudia; Kato, Sumie; Cuello, Mauricio; Parborell, Maria Fernanda Agustina; Irusta, Griselda; Palma, Verónica; Allende, Miguel L.; Owen, Gareth I.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Knockout models have shown that the coagulation system has a role in vascular development and angiogenesis. Herein, we report for the first time that zymogen FX and its active form (FXa) possess anti-angiogenic properties. Both the recombinant FX and FXa inhibit angiogenesis in vitro using endothelial EA.hy926 and human umbilical cord vascular endothelial cells (HUVEC). This effect is dependent on the Gla domain of FX. We demonstrate that FX and FXa use different mechanisms: the use of Rivaroxaban (RX) a specific inhibitor of FXa attenuated its anti-angiogenic properties but did not modify the anti-angiogenic effect of FX. Furthermore, only the anti-angiogenic activity of FXa is PAR-1dependent. Using in vivo models, we show that FX and FXa are anti-angiogenic in the zebrafish intersegmental vasculature (ISV) formation and in the chick embryo chorioallantoic membrane (CAM) assays. Our results provide further evidence for the non-hemostatic functions of FX and FXa and demonstrate for the first time a biological role for the zymogen FX.
Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Gonzalez, Ibeth. Pontificia Universidad Católica de Chile; Chile
Fil: Pinto, Mauricio P.. Pontificia Universidad Católica de Chile; Chile
Fil: Arce, Maximiliano. Pontificia Universidad Católica de Chile; Chile
Fil: Valenzuela, Rodrigo. Pontificia Universidad Católica de Chile; Chile
Fil: Aranda, Evelyn. Pontificia Universidad Católica de Chile; Chile
Fil: Elliot, Matias. Universidad de Chile. Facultad de Ciencias; Chile
Fil: Alvarez, Marjorie. Universidad de Chile. Facultad de Ciencias; Chile
Fil: Henriquez, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Velazquez, Ethel. Pontificia Universidad Católica de Chile; Chile
Fil: Orge, Felipe. Pontificia Universidad Católica de Chile; Chile
Fil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; Chile
Fil: Gonzalez, Pamela. Pontificia Universidad Católica de Chile; Chile
Fil: Villalon, Manuel. Pontificia Universidad Católica de Chile; Chile
Fil: Cautivo, Kelly M.. Pontificia Universidad Católica de Chile; Chile
Fil: Kalergis, Alexis M.. Pontificia Universidad Católica de Chile; Chile
Fil: Pereira, Karla. Pontificia Universidad Católica de Chile; Chile
Fil: Mendoza, Camila. Pontificia Universidad Católica de Chile; Chile
Fil: Saez, Claudia. Pontificia Universidad Católica de Chile; Chile
Fil: Kato, Sumie. Pontificia Universidad Católica de Chile; Chile
Fil: Cuello, Mauricio. Pontificia Universidad Católica de Chile; Chile
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Palma, Verónica. Universidad de Chile. Facultad de Ciencias; Chile
Fil: Allende, Miguel L.. Universidad de Chile. Facultad de Ciencias; Chile
Fil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; Chile. Biomedical Research Consortium Chile; Chile - Materia
-
Angiogenesis Inhibitors
Coagulation
Fx
Fxa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6443
Ver los metadatos del registro completo
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Independent anti-angiogenic capacities of coagulation factors X and XaLange, SoledadGonzalez, IbethPinto, Mauricio P.Arce, MaximilianoValenzuela, RodrigoAranda, EvelynElliot, MatiasAlvarez, MarjorieHenriquez, SoledadVelazquez, EthelOrge, FelipeOliva, BárbaraGonzalez, PamelaVillalon, ManuelCautivo, Kelly M.Kalergis, Alexis M.Pereira, KarlaMendoza, CamilaSaez, ClaudiaKato, SumieCuello, MauricioParborell, Maria Fernanda AgustinaIrusta, GriseldaPalma, VerónicaAllende, Miguel L.Owen, Gareth I.Angiogenesis InhibitorsCoagulationFxFxahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Knockout models have shown that the coagulation system has a role in vascular development and angiogenesis. Herein, we report for the first time that zymogen FX and its active form (FXa) possess anti-angiogenic properties. Both the recombinant FX and FXa inhibit angiogenesis in vitro using endothelial EA.hy926 and human umbilical cord vascular endothelial cells (HUVEC). This effect is dependent on the Gla domain of FX. We demonstrate that FX and FXa use different mechanisms: the use of Rivaroxaban (RX) a specific inhibitor of FXa attenuated its anti-angiogenic properties but did not modify the anti-angiogenic effect of FX. Furthermore, only the anti-angiogenic activity of FXa is PAR-1dependent. Using in vivo models, we show that FX and FXa are anti-angiogenic in the zebrafish intersegmental vasculature (ISV) formation and in the chick embryo chorioallantoic membrane (CAM) assays. Our results provide further evidence for the non-hemostatic functions of FX and FXa and demonstrate for the first time a biological role for the zymogen FX.Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; ChileFil: Gonzalez, Ibeth. Pontificia Universidad Católica de Chile; ChileFil: Pinto, Mauricio P.. Pontificia Universidad Católica de Chile; ChileFil: Arce, Maximiliano. Pontificia Universidad Católica de Chile; ChileFil: Valenzuela, Rodrigo. Pontificia Universidad Católica de Chile; ChileFil: Aranda, Evelyn. Pontificia Universidad Católica de Chile; ChileFil: Elliot, Matias. Universidad de Chile. Facultad de Ciencias; ChileFil: Alvarez, Marjorie. Universidad de Chile. Facultad de Ciencias; ChileFil: Henriquez, Soledad. Pontificia Universidad Católica de Chile; ChileFil: Velazquez, Ethel. Pontificia Universidad Católica de Chile; ChileFil: Orge, Felipe. Pontificia Universidad Católica de Chile; ChileFil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; ChileFil: Gonzalez, Pamela. Pontificia Universidad Católica de Chile; ChileFil: Villalon, Manuel. Pontificia Universidad Católica de Chile; ChileFil: Cautivo, Kelly M.. Pontificia Universidad Católica de Chile; ChileFil: Kalergis, Alexis M.. Pontificia Universidad Católica de Chile; ChileFil: Pereira, Karla. Pontificia Universidad Católica de Chile; ChileFil: Mendoza, Camila. Pontificia Universidad Católica de Chile; ChileFil: Saez, Claudia. Pontificia Universidad Católica de Chile; ChileFil: Kato, Sumie. Pontificia Universidad Católica de Chile; ChileFil: Cuello, Mauricio. Pontificia Universidad Católica de Chile; ChileFil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Palma, Verónica. Universidad de Chile. Facultad de Ciencias; ChileFil: Allende, Miguel L.. Universidad de Chile. Facultad de Ciencias; ChileFil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; Chile. Biomedical Research Consortium Chile; ChileWiley2014-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6443Lange, Soledad; Gonzalez, Ibeth; Pinto, Mauricio P.; Arce, Maximiliano; Valenzuela, Rodrigo; et al.; Independent anti-angiogenic capacities of coagulation factors X and Xa; Wiley; Journal of Cellular Physiology; 229; 11; 29-7-2014; 1673-16800021-95411097-4652enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.24612info:eu-repo/semantics/altIdentifier/pmid/24615682info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24612/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:17Zoai:ri.conicet.gov.ar:11336/6443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:17.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| title |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| spellingShingle |
Independent anti-angiogenic capacities of coagulation factors X and Xa Lange, Soledad Angiogenesis Inhibitors Coagulation Fx Fxa |
| title_short |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| title_full |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| title_fullStr |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| title_full_unstemmed |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| title_sort |
Independent anti-angiogenic capacities of coagulation factors X and Xa |
| dc.creator.none.fl_str_mv |
Lange, Soledad Gonzalez, Ibeth Pinto, Mauricio P. Arce, Maximiliano Valenzuela, Rodrigo Aranda, Evelyn Elliot, Matias Alvarez, Marjorie Henriquez, Soledad Velazquez, Ethel Orge, Felipe Oliva, Bárbara Gonzalez, Pamela Villalon, Manuel Cautivo, Kelly M. Kalergis, Alexis M. Pereira, Karla Mendoza, Camila Saez, Claudia Kato, Sumie Cuello, Mauricio Parborell, Maria Fernanda Agustina Irusta, Griselda Palma, Verónica Allende, Miguel L. Owen, Gareth I. |
| author |
Lange, Soledad |
| author_facet |
Lange, Soledad Gonzalez, Ibeth Pinto, Mauricio P. Arce, Maximiliano Valenzuela, Rodrigo Aranda, Evelyn Elliot, Matias Alvarez, Marjorie Henriquez, Soledad Velazquez, Ethel Orge, Felipe Oliva, Bárbara Gonzalez, Pamela Villalon, Manuel Cautivo, Kelly M. Kalergis, Alexis M. Pereira, Karla Mendoza, Camila Saez, Claudia Kato, Sumie Cuello, Mauricio Parborell, Maria Fernanda Agustina Irusta, Griselda Palma, Verónica Allende, Miguel L. Owen, Gareth I. |
| author_role |
author |
| author2 |
Gonzalez, Ibeth Pinto, Mauricio P. Arce, Maximiliano Valenzuela, Rodrigo Aranda, Evelyn Elliot, Matias Alvarez, Marjorie Henriquez, Soledad Velazquez, Ethel Orge, Felipe Oliva, Bárbara Gonzalez, Pamela Villalon, Manuel Cautivo, Kelly M. Kalergis, Alexis M. Pereira, Karla Mendoza, Camila Saez, Claudia Kato, Sumie Cuello, Mauricio Parborell, Maria Fernanda Agustina Irusta, Griselda Palma, Verónica Allende, Miguel L. Owen, Gareth I. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Angiogenesis Inhibitors Coagulation Fx Fxa |
| topic |
Angiogenesis Inhibitors Coagulation Fx Fxa |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Knockout models have shown that the coagulation system has a role in vascular development and angiogenesis. Herein, we report for the first time that zymogen FX and its active form (FXa) possess anti-angiogenic properties. Both the recombinant FX and FXa inhibit angiogenesis in vitro using endothelial EA.hy926 and human umbilical cord vascular endothelial cells (HUVEC). This effect is dependent on the Gla domain of FX. We demonstrate that FX and FXa use different mechanisms: the use of Rivaroxaban (RX) a specific inhibitor of FXa attenuated its anti-angiogenic properties but did not modify the anti-angiogenic effect of FX. Furthermore, only the anti-angiogenic activity of FXa is PAR-1dependent. Using in vivo models, we show that FX and FXa are anti-angiogenic in the zebrafish intersegmental vasculature (ISV) formation and in the chick embryo chorioallantoic membrane (CAM) assays. Our results provide further evidence for the non-hemostatic functions of FX and FXa and demonstrate for the first time a biological role for the zymogen FX. Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; Chile Fil: Gonzalez, Ibeth. Pontificia Universidad Católica de Chile; Chile Fil: Pinto, Mauricio P.. Pontificia Universidad Católica de Chile; Chile Fil: Arce, Maximiliano. Pontificia Universidad Católica de Chile; Chile Fil: Valenzuela, Rodrigo. Pontificia Universidad Católica de Chile; Chile Fil: Aranda, Evelyn. Pontificia Universidad Católica de Chile; Chile Fil: Elliot, Matias. Universidad de Chile. Facultad de Ciencias; Chile Fil: Alvarez, Marjorie. Universidad de Chile. Facultad de Ciencias; Chile Fil: Henriquez, Soledad. Pontificia Universidad Católica de Chile; Chile Fil: Velazquez, Ethel. Pontificia Universidad Católica de Chile; Chile Fil: Orge, Felipe. Pontificia Universidad Católica de Chile; Chile Fil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; Chile Fil: Gonzalez, Pamela. Pontificia Universidad Católica de Chile; Chile Fil: Villalon, Manuel. Pontificia Universidad Católica de Chile; Chile Fil: Cautivo, Kelly M.. Pontificia Universidad Católica de Chile; Chile Fil: Kalergis, Alexis M.. Pontificia Universidad Católica de Chile; Chile Fil: Pereira, Karla. Pontificia Universidad Católica de Chile; Chile Fil: Mendoza, Camila. Pontificia Universidad Católica de Chile; Chile Fil: Saez, Claudia. Pontificia Universidad Católica de Chile; Chile Fil: Kato, Sumie. Pontificia Universidad Católica de Chile; Chile Fil: Cuello, Mauricio. Pontificia Universidad Católica de Chile; Chile Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Palma, Verónica. Universidad de Chile. Facultad de Ciencias; Chile Fil: Allende, Miguel L.. Universidad de Chile. Facultad de Ciencias; Chile Fil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; Chile. Biomedical Research Consortium Chile; Chile |
| description |
Knockout models have shown that the coagulation system has a role in vascular development and angiogenesis. Herein, we report for the first time that zymogen FX and its active form (FXa) possess anti-angiogenic properties. Both the recombinant FX and FXa inhibit angiogenesis in vitro using endothelial EA.hy926 and human umbilical cord vascular endothelial cells (HUVEC). This effect is dependent on the Gla domain of FX. We demonstrate that FX and FXa use different mechanisms: the use of Rivaroxaban (RX) a specific inhibitor of FXa attenuated its anti-angiogenic properties but did not modify the anti-angiogenic effect of FX. Furthermore, only the anti-angiogenic activity of FXa is PAR-1dependent. Using in vivo models, we show that FX and FXa are anti-angiogenic in the zebrafish intersegmental vasculature (ISV) formation and in the chick embryo chorioallantoic membrane (CAM) assays. Our results provide further evidence for the non-hemostatic functions of FX and FXa and demonstrate for the first time a biological role for the zymogen FX. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-07-29 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/6443 Lange, Soledad; Gonzalez, Ibeth; Pinto, Mauricio P.; Arce, Maximiliano; Valenzuela, Rodrigo; et al.; Independent anti-angiogenic capacities of coagulation factors X and Xa; Wiley; Journal of Cellular Physiology; 229; 11; 29-7-2014; 1673-1680 0021-9541 1097-4652 |
| url |
http://hdl.handle.net/11336/6443 |
| identifier_str_mv |
Lange, Soledad; Gonzalez, Ibeth; Pinto, Mauricio P.; Arce, Maximiliano; Valenzuela, Rodrigo; et al.; Independent anti-angiogenic capacities of coagulation factors X and Xa; Wiley; Journal of Cellular Physiology; 229; 11; 29-7-2014; 1673-1680 0021-9541 1097-4652 |
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eng |
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eng |
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