Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts

Autores
Cogoi, Laura Carolina; Marrassini, Carla; Saint Martin, Elina Malén; Alonso Ibañez, María del Rosario; Filip, Rosana; Anesini, Claudia Alejandra
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: Ilex paraguariensis (Aquifoleaceae) is cultivated to produce “yerba mate”. Due to its nutritional, energizing, hypoglycemic and antioxidant effects, it is used in the elabo ration of food, pharmaceuticals, and cosmetics. The oxidative stress related to protein glycation and production of advanced glycation end products (AGEs) leads to the develop ment of several diseases. The objective of this work was to compare the antioxidant and anti-AGEs activity of a decoction of fruits (F) and leaves (L). Methods: The antioxidant activity was assayed by the DPPH assay and the inhibition o egg yolk lipid peroxidation (ILP), and anti-AGEs activity, through the inhibition of the forma tion of fructosamine (IF), β-amyloid (Iβ), protein carbonylation (IC) and AGEs (IA). Polyphe nols were quantified by HPLC. Results: Maximum response ± SEM: For F 0.01 µg/mL: IF = 42 ± 4%, IC = 17 ± 2% and fo 10 µg/mL: IA = 38 ± 4%, Iβ = 67 ± 7%. For L 0.1 µg/mL: IF = 35 ± 2%, IC = 19 ± 2% and for 100 µg/mL: IA = 26 ± 3%, Iβ = 63.04 ± 2%. The DPPH IC50 = 134.8 ± 14 µg/mL for F and 34.67 ± 3 µg/mL for L. The ILP IC50 = 512.86 ± 50 µg/mL for F and 154.8 ± 15 µg/ mL for L. By HPLC L presented the highest amounts of flavonoids and caffeoylquinic ac ids. F and L showed strong anti-AGEs activity, affecting the early stages of glycation at low concentrations and the late stages of glycation at high concentrations. The highest activ ity for both F and L was seen in the IF and Iβ. F presented the highest anti-AGEs potency L presented the highest antioxidant potency, which was related to the highest content o polyphenols. Conclusion: The fruits of I. paraguariensis could be a source of antioxidant and anti-AGEs compounds to be used with medicinal purposes or as functional food.
Fil: Cogoi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Saint Martin, Elina Malén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Alonso Ibañez, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Filip, Rosana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; Argentina
Fil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
ANTI-AGES ACTIVITY
ANTIOXIDANT ACTIVITY
FRUITS EXTRACT
ILEX PARAGUARIENSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227877
Acceder
id CONICETDig_5b6ff8c8db45bd916bd16547d338654b
oai_identifier_str oai:ri.conicet.gov.ar:11336/227877
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extractsCogoi, Laura CarolinaMarrassini, CarlaSaint Martin, Elina MalénAlonso Ibañez, María del RosarioFilip, RosanaAnesini, Claudia AlejandraANTI-AGES ACTIVITYANTIOXIDANT ACTIVITYFRUITS EXTRACTILEX PARAGUARIENSIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objectives: Ilex paraguariensis (Aquifoleaceae) is cultivated to produce “yerba mate”. Due to its nutritional, energizing, hypoglycemic and antioxidant effects, it is used in the elabo ration of food, pharmaceuticals, and cosmetics. The oxidative stress related to protein glycation and production of advanced glycation end products (AGEs) leads to the develop ment of several diseases. The objective of this work was to compare the antioxidant and anti-AGEs activity of a decoction of fruits (F) and leaves (L). Methods: The antioxidant activity was assayed by the DPPH assay and the inhibition o egg yolk lipid peroxidation (ILP), and anti-AGEs activity, through the inhibition of the forma tion of fructosamine (IF), β-amyloid (Iβ), protein carbonylation (IC) and AGEs (IA). Polyphe nols were quantified by HPLC. Results: Maximum response ± SEM: For F 0.01 µg/mL: IF = 42 ± 4%, IC = 17 ± 2% and fo 10 µg/mL: IA = 38 ± 4%, Iβ = 67 ± 7%. For L 0.1 µg/mL: IF = 35 ± 2%, IC = 19 ± 2% and for 100 µg/mL: IA = 26 ± 3%, Iβ = 63.04 ± 2%. The DPPH IC50 = 134.8 ± 14 µg/mL for F and 34.67 ± 3 µg/mL for L. The ILP IC50 = 512.86 ± 50 µg/mL for F and 154.8 ± 15 µg/ mL for L. By HPLC L presented the highest amounts of flavonoids and caffeoylquinic ac ids. F and L showed strong anti-AGEs activity, affecting the early stages of glycation at low concentrations and the late stages of glycation at high concentrations. The highest activ ity for both F and L was seen in the IF and Iβ. F presented the highest anti-AGEs potency L presented the highest antioxidant potency, which was related to the highest content o polyphenols. Conclusion: The fruits of I. paraguariensis could be a source of antioxidant and anti-AGEs compounds to be used with medicinal purposes or as functional food.Fil: Cogoi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Saint Martin, Elina Malén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Alonso Ibañez, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Filip, Rosana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; ArgentinaFil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaKorean Pharmacopuncture Institute2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227877Cogoi, Laura Carolina; Marrassini, Carla; Saint Martin, Elina Malén; Alonso Ibañez, María del Rosario; Filip, Rosana; et al.; Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts; Korean Pharmacopuncture Institute; Journal of Pharmacopuncture; 26; 4; 12-2023; 338-3472093-69662234-6856CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2023.26.4.338info:eu-repo/semantics/altIdentifier/doi/10.3831/KPI.2023.26.4.338info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:12Zoai:ri.conicet.gov.ar:11336/227877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:13.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
title Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
spellingShingle Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
Cogoi, Laura Carolina
ANTI-AGES ACTIVITY
ANTIOXIDANT ACTIVITY
FRUITS EXTRACT
ILEX PARAGUARIENSIS
title_short Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
title_full Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
title_fullStr Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
title_full_unstemmed Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
title_sort Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts
dc.creator.none.fl_str_mv Cogoi, Laura Carolina
Marrassini, Carla
Saint Martin, Elina Malén
Alonso Ibañez, María del Rosario
Filip, Rosana
Anesini, Claudia Alejandra
author Cogoi, Laura Carolina
author_facet Cogoi, Laura Carolina
Marrassini, Carla
Saint Martin, Elina Malén
Alonso Ibañez, María del Rosario
Filip, Rosana
Anesini, Claudia Alejandra
author_role author
author2 Marrassini, Carla
Saint Martin, Elina Malén
Alonso Ibañez, María del Rosario
Filip, Rosana
Anesini, Claudia Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTI-AGES ACTIVITY
ANTIOXIDANT ACTIVITY
FRUITS EXTRACT
ILEX PARAGUARIENSIS
topic ANTI-AGES ACTIVITY
ANTIOXIDANT ACTIVITY
FRUITS EXTRACT
ILEX PARAGUARIENSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: Ilex paraguariensis (Aquifoleaceae) is cultivated to produce “yerba mate”. Due to its nutritional, energizing, hypoglycemic and antioxidant effects, it is used in the elabo ration of food, pharmaceuticals, and cosmetics. The oxidative stress related to protein glycation and production of advanced glycation end products (AGEs) leads to the develop ment of several diseases. The objective of this work was to compare the antioxidant and anti-AGEs activity of a decoction of fruits (F) and leaves (L). Methods: The antioxidant activity was assayed by the DPPH assay and the inhibition o egg yolk lipid peroxidation (ILP), and anti-AGEs activity, through the inhibition of the forma tion of fructosamine (IF), β-amyloid (Iβ), protein carbonylation (IC) and AGEs (IA). Polyphe nols were quantified by HPLC. Results: Maximum response ± SEM: For F 0.01 µg/mL: IF = 42 ± 4%, IC = 17 ± 2% and fo 10 µg/mL: IA = 38 ± 4%, Iβ = 67 ± 7%. For L 0.1 µg/mL: IF = 35 ± 2%, IC = 19 ± 2% and for 100 µg/mL: IA = 26 ± 3%, Iβ = 63.04 ± 2%. The DPPH IC50 = 134.8 ± 14 µg/mL for F and 34.67 ± 3 µg/mL for L. The ILP IC50 = 512.86 ± 50 µg/mL for F and 154.8 ± 15 µg/ mL for L. By HPLC L presented the highest amounts of flavonoids and caffeoylquinic ac ids. F and L showed strong anti-AGEs activity, affecting the early stages of glycation at low concentrations and the late stages of glycation at high concentrations. The highest activ ity for both F and L was seen in the IF and Iβ. F presented the highest anti-AGEs potency L presented the highest antioxidant potency, which was related to the highest content o polyphenols. Conclusion: The fruits of I. paraguariensis could be a source of antioxidant and anti-AGEs compounds to be used with medicinal purposes or as functional food.
Fil: Cogoi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marrassini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Saint Martin, Elina Malén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Alonso Ibañez, María del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Filip, Rosana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; Argentina
Fil: Anesini, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description Objectives: Ilex paraguariensis (Aquifoleaceae) is cultivated to produce “yerba mate”. Due to its nutritional, energizing, hypoglycemic and antioxidant effects, it is used in the elabo ration of food, pharmaceuticals, and cosmetics. The oxidative stress related to protein glycation and production of advanced glycation end products (AGEs) leads to the develop ment of several diseases. The objective of this work was to compare the antioxidant and anti-AGEs activity of a decoction of fruits (F) and leaves (L). Methods: The antioxidant activity was assayed by the DPPH assay and the inhibition o egg yolk lipid peroxidation (ILP), and anti-AGEs activity, through the inhibition of the forma tion of fructosamine (IF), β-amyloid (Iβ), protein carbonylation (IC) and AGEs (IA). Polyphe nols were quantified by HPLC. Results: Maximum response ± SEM: For F 0.01 µg/mL: IF = 42 ± 4%, IC = 17 ± 2% and fo 10 µg/mL: IA = 38 ± 4%, Iβ = 67 ± 7%. For L 0.1 µg/mL: IF = 35 ± 2%, IC = 19 ± 2% and for 100 µg/mL: IA = 26 ± 3%, Iβ = 63.04 ± 2%. The DPPH IC50 = 134.8 ± 14 µg/mL for F and 34.67 ± 3 µg/mL for L. The ILP IC50 = 512.86 ± 50 µg/mL for F and 154.8 ± 15 µg/ mL for L. By HPLC L presented the highest amounts of flavonoids and caffeoylquinic ac ids. F and L showed strong anti-AGEs activity, affecting the early stages of glycation at low concentrations and the late stages of glycation at high concentrations. The highest activ ity for both F and L was seen in the IF and Iβ. F presented the highest anti-AGEs potency L presented the highest antioxidant potency, which was related to the highest content o polyphenols. Conclusion: The fruits of I. paraguariensis could be a source of antioxidant and anti-AGEs compounds to be used with medicinal purposes or as functional food.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227877
Cogoi, Laura Carolina; Marrassini, Carla; Saint Martin, Elina Malén; Alonso Ibañez, María del Rosario; Filip, Rosana; et al.; Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts; Korean Pharmacopuncture Institute; Journal of Pharmacopuncture; 26; 4; 12-2023; 338-347
2093-6966
2234-6856
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227877
identifier_str_mv Cogoi, Laura Carolina; Marrassini, Carla; Saint Martin, Elina Malén; Alonso Ibañez, María del Rosario; Filip, Rosana; et al.; Inhibition of Glycation End Products Formation and Antioxidant Activities of Ilex paraguariensis: comparative study of fruit and leaves extracts; Korean Pharmacopuncture Institute; Journal of Pharmacopuncture; 26; 4; 12-2023; 338-347
2093-6966
2234-6856
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2023.26.4.338
info:eu-repo/semantics/altIdentifier/doi/10.3831/KPI.2023.26.4.338
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Korean Pharmacopuncture Institute
publisher.none.fl_str_mv Korean Pharmacopuncture Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082859767431168
score 13.22299

Publicaciones similares