Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test

Autores
Bidau, Claudio Juan; Amat, Anibal Gumersindo; Yajia, Marta Esther; Marti, Dardo Andrea; Riglos, Ana G.; Silvestroni, Aurelio
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infusions and concoctions of Ilex paraguariensis are used as medicinal, nutritional and stimulant beverages in southern South America. Crop production is about 300,000 tons/year in Argentina, where the consumption rate reaches 5–9 kg/capita/year. In this study, we assessed the cytotoxicity of aqueous extracts of I. paraguariensis in the Allium test and Artemia salina microwell test. The extracts were prepared from commercial and “home processed” (laboratory) and were tested at concentrations of 5–40 g/l. Both extracts significantly decreased root growth and the mitotic index (MI). These effects were greater for the commercial material for which concentrations ≥10 g/l virtually abolished mitosis. The disturbance of mitotic behaviour was significant at 5–10 g/l of the “home-processed” product and included c-mitotic phenomena (over-condensed and disorganized metaphases, sticky metaphases, arrested anaphases, binucleated interphases) which could contribute to the increase in metaphase and anaphase indexes. None of the extracts were cytotoxic in the Artemia salina test. It is concluded that the Allium test is adequate for a preliminary screening of genotoxicity of medicinal plants and that genotoxic effects can be increased by the commercial manipulation of the raw product.
Fil: Bidau, Claudio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Amat, Anibal Gumersindo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Yajia, Marta Esther. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Riglos, Ana G.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Silvestroni, Aurelio. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Materia
ALLIUM TEST
ANTIMITOTIC ACTIVITY
BIOASSAY
GENOTOXICITY
ILEX PARAGUARIENSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47738
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47738
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium TestBidau, Claudio JuanAmat, Anibal GumersindoYajia, Marta EstherMarti, Dardo AndreaRiglos, Ana G.Silvestroni, AurelioALLIUM TESTANTIMITOTIC ACTIVITYBIOASSAYGENOTOXICITYILEX PARAGUARIENSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Infusions and concoctions of Ilex paraguariensis are used as medicinal, nutritional and stimulant beverages in southern South America. Crop production is about 300,000 tons/year in Argentina, where the consumption rate reaches 5–9 kg/capita/year. In this study, we assessed the cytotoxicity of aqueous extracts of I. paraguariensis in the Allium test and Artemia salina microwell test. The extracts were prepared from commercial and “home processed” (laboratory) and were tested at concentrations of 5–40 g/l. Both extracts significantly decreased root growth and the mitotic index (MI). These effects were greater for the commercial material for which concentrations ≥10 g/l virtually abolished mitosis. The disturbance of mitotic behaviour was significant at 5–10 g/l of the “home-processed” product and included c-mitotic phenomena (over-condensed and disorganized metaphases, sticky metaphases, arrested anaphases, binucleated interphases) which could contribute to the increase in metaphase and anaphase indexes. None of the extracts were cytotoxic in the Artemia salina test. It is concluded that the Allium test is adequate for a preliminary screening of genotoxicity of medicinal plants and that genotoxic effects can be increased by the commercial manipulation of the raw product.Fil: Bidau, Claudio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Amat, Anibal Gumersindo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Yajia, Marta Esther. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Riglos, Ana G.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Silvestroni, Aurelio. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaJapan Mendel Society2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47738Bidau, Claudio Juan; Amat, Anibal Gumersindo; Yajia, Marta Esther; Marti, Dardo Andrea; Riglos, Ana G.; et al.; Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test; Japan Mendel Society; Cytologia; 69; 2; 12-2004; 109-1170011-45451348-7019CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/cytologia/69/2/69_2_109/_article/-char/eninfo:eu-repo/semantics/altIdentifier/doi/10.1508/cytologia.69.109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:04Zoai:ri.conicet.gov.ar:11336/47738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:04.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
title Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
spellingShingle Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
Bidau, Claudio Juan
ALLIUM TEST
ANTIMITOTIC ACTIVITY
BIOASSAY
GENOTOXICITY
ILEX PARAGUARIENSIS
title_short Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
title_full Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
title_fullStr Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
title_full_unstemmed Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
title_sort Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test
dc.creator.none.fl_str_mv Bidau, Claudio Juan
Amat, Anibal Gumersindo
Yajia, Marta Esther
Marti, Dardo Andrea
Riglos, Ana G.
Silvestroni, Aurelio
author Bidau, Claudio Juan
author_facet Bidau, Claudio Juan
Amat, Anibal Gumersindo
Yajia, Marta Esther
Marti, Dardo Andrea
Riglos, Ana G.
Silvestroni, Aurelio
author_role author
author2 Amat, Anibal Gumersindo
Yajia, Marta Esther
Marti, Dardo Andrea
Riglos, Ana G.
Silvestroni, Aurelio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALLIUM TEST
ANTIMITOTIC ACTIVITY
BIOASSAY
GENOTOXICITY
ILEX PARAGUARIENSIS
topic ALLIUM TEST
ANTIMITOTIC ACTIVITY
BIOASSAY
GENOTOXICITY
ILEX PARAGUARIENSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Infusions and concoctions of Ilex paraguariensis are used as medicinal, nutritional and stimulant beverages in southern South America. Crop production is about 300,000 tons/year in Argentina, where the consumption rate reaches 5–9 kg/capita/year. In this study, we assessed the cytotoxicity of aqueous extracts of I. paraguariensis in the Allium test and Artemia salina microwell test. The extracts were prepared from commercial and “home processed” (laboratory) and were tested at concentrations of 5–40 g/l. Both extracts significantly decreased root growth and the mitotic index (MI). These effects were greater for the commercial material for which concentrations ≥10 g/l virtually abolished mitosis. The disturbance of mitotic behaviour was significant at 5–10 g/l of the “home-processed” product and included c-mitotic phenomena (over-condensed and disorganized metaphases, sticky metaphases, arrested anaphases, binucleated interphases) which could contribute to the increase in metaphase and anaphase indexes. None of the extracts were cytotoxic in the Artemia salina test. It is concluded that the Allium test is adequate for a preliminary screening of genotoxicity of medicinal plants and that genotoxic effects can be increased by the commercial manipulation of the raw product.
Fil: Bidau, Claudio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Amat, Anibal Gumersindo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Yajia, Marta Esther. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Riglos, Ana G.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Silvestroni, Aurelio. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
description Infusions and concoctions of Ilex paraguariensis are used as medicinal, nutritional and stimulant beverages in southern South America. Crop production is about 300,000 tons/year in Argentina, where the consumption rate reaches 5–9 kg/capita/year. In this study, we assessed the cytotoxicity of aqueous extracts of I. paraguariensis in the Allium test and Artemia salina microwell test. The extracts were prepared from commercial and “home processed” (laboratory) and were tested at concentrations of 5–40 g/l. Both extracts significantly decreased root growth and the mitotic index (MI). These effects were greater for the commercial material for which concentrations ≥10 g/l virtually abolished mitosis. The disturbance of mitotic behaviour was significant at 5–10 g/l of the “home-processed” product and included c-mitotic phenomena (over-condensed and disorganized metaphases, sticky metaphases, arrested anaphases, binucleated interphases) which could contribute to the increase in metaphase and anaphase indexes. None of the extracts were cytotoxic in the Artemia salina test. It is concluded that the Allium test is adequate for a preliminary screening of genotoxicity of medicinal plants and that genotoxic effects can be increased by the commercial manipulation of the raw product.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47738
Bidau, Claudio Juan; Amat, Anibal Gumersindo; Yajia, Marta Esther; Marti, Dardo Andrea; Riglos, Ana G.; et al.; Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test; Japan Mendel Society; Cytologia; 69; 2; 12-2004; 109-117
0011-4545
1348-7019
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47738
identifier_str_mv Bidau, Claudio Juan; Amat, Anibal Gumersindo; Yajia, Marta Esther; Marti, Dardo Andrea; Riglos, Ana G.; et al.; Evaluation of the Genotoxicity of Aqueous Extracts of Ilex paraguariensis St. Hil. (Aquifoliaceae) Using the Allium Test; Japan Mendel Society; Cytologia; 69; 2; 12-2004; 109-117
0011-4545
1348-7019
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/cytologia/69/2/69_2_109/_article/-char/en
info:eu-repo/semantics/altIdentifier/doi/10.1508/cytologia.69.109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Japan Mendel Society
publisher.none.fl_str_mv Japan Mendel Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269781340717056
score 13.13397

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