Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn

Autores
Soto Rueda, Eliana Marcela; Restrepo, Rodrigo Andrés; Loango, Nelsy; Restrepo, Beatriz; Landazuri, Patricia; Rai, Mahendra
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majoranaa low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.
Fil: Soto Rueda, Eliana Marcela. Universidad del Quindio; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Restrepo, Rodrigo Andrés. Universidad del Quindio; Colombia
Fil: Loango, Nelsy. Universidad del Quindio; Colombia
Fil: Restrepo, Beatriz. Universidad del Quindio; Colombia
Fil: Landazuri, Patricia. Universidad del Quindio; Colombia
Fil: Rai, Mahendra. Amravati University; India
Materia
ANTICANCER
ANTIOXIDANT
CYTOTOXIC
EXTRACT
FLAVONOIDS
O. MARJORANA
PHENOLIC COMPOUDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125507
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/125507
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana LinnSoto Rueda, Eliana MarcelaRestrepo, Rodrigo AndrésLoango, NelsyRestrepo, BeatrizLandazuri, PatriciaRai, MahendraANTICANCERANTIOXIDANTCYTOTOXICEXTRACTFLAVONOIDSO. MARJORANAPHENOLIC COMPOUDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majoranaa low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.Fil: Soto Rueda, Eliana Marcela. Universidad del Quindio; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Restrepo, Rodrigo Andrés. Universidad del Quindio; ColombiaFil: Loango, Nelsy. Universidad del Quindio; ColombiaFil: Restrepo, Beatriz. Universidad del Quindio; ColombiaFil: Landazuri, Patricia. Universidad del Quindio; ColombiaFil: Rai, Mahendra. Amravati University; IndiaIndian Society for Education and Environment2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125507Soto Rueda, Eliana Marcela; Restrepo, Rodrigo Andrés; Loango, Nelsy; Restrepo, Beatriz; Landazuri, Patricia; et al.; Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn; Indian Society for Education and Environment; Indian Journal of Science and Technology; 12; 1; 1-2019; 1-60974-5645CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17485/ijst/2019/v12i1/139859info:eu-repo/semantics/altIdentifier/url/https://indjst.org/articles/potential-anti-cancer-cytotoxic-and-antioxidant-of-polar-and-non-polar-extracts-of-origanummajorana-linninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:04:23Zoai:ri.conicet.gov.ar:11336/125507instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:04:23.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
title Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
spellingShingle Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
Soto Rueda, Eliana Marcela
ANTICANCER
ANTIOXIDANT
CYTOTOXIC
EXTRACT
FLAVONOIDS
O. MARJORANA
PHENOLIC COMPOUDS
title_short Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
title_full Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
title_fullStr Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
title_full_unstemmed Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
title_sort Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
dc.creator.none.fl_str_mv Soto Rueda, Eliana Marcela
Restrepo, Rodrigo Andrés
Loango, Nelsy
Restrepo, Beatriz
Landazuri, Patricia
Rai, Mahendra
author Soto Rueda, Eliana Marcela
author_facet Soto Rueda, Eliana Marcela
Restrepo, Rodrigo Andrés
Loango, Nelsy
Restrepo, Beatriz
Landazuri, Patricia
Rai, Mahendra
author_role author
author2 Restrepo, Rodrigo Andrés
Loango, Nelsy
Restrepo, Beatriz
Landazuri, Patricia
Rai, Mahendra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTICANCER
ANTIOXIDANT
CYTOTOXIC
EXTRACT
FLAVONOIDS
O. MARJORANA
PHENOLIC COMPOUDS
topic ANTICANCER
ANTIOXIDANT
CYTOTOXIC
EXTRACT
FLAVONOIDS
O. MARJORANA
PHENOLIC COMPOUDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majoranaa low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.
Fil: Soto Rueda, Eliana Marcela. Universidad del Quindio; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Restrepo, Rodrigo Andrés. Universidad del Quindio; Colombia
Fil: Loango, Nelsy. Universidad del Quindio; Colombia
Fil: Restrepo, Beatriz. Universidad del Quindio; Colombia
Fil: Landazuri, Patricia. Universidad del Quindio; Colombia
Fil: Rai, Mahendra. Amravati University; India
description Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majoranaa low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125507
Soto Rueda, Eliana Marcela; Restrepo, Rodrigo Andrés; Loango, Nelsy; Restrepo, Beatriz; Landazuri, Patricia; et al.; Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn; Indian Society for Education and Environment; Indian Journal of Science and Technology; 12; 1; 1-2019; 1-6
0974-5645
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125507
identifier_str_mv Soto Rueda, Eliana Marcela; Restrepo, Rodrigo Andrés; Loango, Nelsy; Restrepo, Beatriz; Landazuri, Patricia; et al.; Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn; Indian Society for Education and Environment; Indian Journal of Science and Technology; 12; 1; 1-2019; 1-6
0974-5645
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.17485/ijst/2019/v12i1/139859
info:eu-repo/semantics/altIdentifier/url/https://indjst.org/articles/potential-anti-cancer-cytotoxic-and-antioxidant-of-polar-and-non-polar-extracts-of-origanummajorana-linn
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Indian Society for Education and Environment
publisher.none.fl_str_mv Indian Society for Education and Environment
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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