Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper

Autores
Fonovich, Teresa Mabel; Perez Coll, Cristina Silvia; Fridman, Osvaldo; D'eramo, Jose Luis; Herkovits, Jorge
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report phospholipid changes in Rhinella arenarum embryos after applying three acclimation protocols to copper between 40 and 420 ng L− 1. The lower and higher acclimation treatments resulted in embryos' enhanced resistance to this metal. Phospholipid remodeling activity, evident through arachidonic acid radioactivity incorporation increase in phosphatidylcholine (PC) and sphingomyelin (SPH) fractions, was registered in embryos acclimated to the intermediate exposure condition. Concomitantly, a decrease in phosphatidic acid fraction (PA) was registered in the higher acclimation condition. PC/PE radioactivity ratio increased both for medium and high acclimation conditions from 0.493 in control embryos to 1.378 and 1.032 respectively. Phospholipid changes could be relevant for changes in membrane features associated with low level exposures to copper, preparing the embryo for a higher resistance to this metal. The increased resistance to copper could also be associated with both an increase in metallothioneins concentration, as registered with HPLC in all the acclimation conditions, and an increase in the copper bound to the third fraction of metallothioneins separated by this method. Our results point out that even very low level exposure to copper results in phospholipid metabolism changes that could be relevant for the acclimation phenomena.
Fil: Fonovich, Teresa Mabel. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: D'eramo, Jose Luis. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Phospholipids
Acclimation
Amphibian
Copper
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46801
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copperFonovich, Teresa MabelPerez Coll, Cristina SilviaFridman, OsvaldoD'eramo, Jose LuisHerkovits, JorgePhospholipidsAcclimationAmphibianCopperhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report phospholipid changes in Rhinella arenarum embryos after applying three acclimation protocols to copper between 40 and 420 ng L− 1. The lower and higher acclimation treatments resulted in embryos' enhanced resistance to this metal. Phospholipid remodeling activity, evident through arachidonic acid radioactivity incorporation increase in phosphatidylcholine (PC) and sphingomyelin (SPH) fractions, was registered in embryos acclimated to the intermediate exposure condition. Concomitantly, a decrease in phosphatidic acid fraction (PA) was registered in the higher acclimation condition. PC/PE radioactivity ratio increased both for medium and high acclimation conditions from 0.493 in control embryos to 1.378 and 1.032 respectively. Phospholipid changes could be relevant for changes in membrane features associated with low level exposures to copper, preparing the embryo for a higher resistance to this metal. The increased resistance to copper could also be associated with both an increase in metallothioneins concentration, as registered with HPLC in all the acclimation conditions, and an increase in the copper bound to the third fraction of metallothioneins separated by this method. Our results point out that even very low level exposure to copper results in phospholipid metabolism changes that could be relevant for the acclimation phenomena.Fil: Fonovich, Teresa Mabel. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: D'eramo, Jose Luis. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Inc2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46801Fonovich, Teresa Mabel; Perez Coll, Cristina Silvia; Fridman, Osvaldo; D'eramo, Jose Luis; Herkovits, Jorge; Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 10-161532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2016.06.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045616300722info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:02Zoai:ri.conicet.gov.ar:11336/46801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:02.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
title Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
spellingShingle Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
Fonovich, Teresa Mabel
Phospholipids
Acclimation
Amphibian
Copper
title_short Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
title_full Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
title_fullStr Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
title_full_unstemmed Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
title_sort Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper
dc.creator.none.fl_str_mv Fonovich, Teresa Mabel
Perez Coll, Cristina Silvia
Fridman, Osvaldo
D'eramo, Jose Luis
Herkovits, Jorge
author Fonovich, Teresa Mabel
author_facet Fonovich, Teresa Mabel
Perez Coll, Cristina Silvia
Fridman, Osvaldo
D'eramo, Jose Luis
Herkovits, Jorge
author_role author
author2 Perez Coll, Cristina Silvia
Fridman, Osvaldo
D'eramo, Jose Luis
Herkovits, Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv Phospholipids
Acclimation
Amphibian
Copper
topic Phospholipids
Acclimation
Amphibian
Copper
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report phospholipid changes in Rhinella arenarum embryos after applying three acclimation protocols to copper between 40 and 420 ng L− 1. The lower and higher acclimation treatments resulted in embryos' enhanced resistance to this metal. Phospholipid remodeling activity, evident through arachidonic acid radioactivity incorporation increase in phosphatidylcholine (PC) and sphingomyelin (SPH) fractions, was registered in embryos acclimated to the intermediate exposure condition. Concomitantly, a decrease in phosphatidic acid fraction (PA) was registered in the higher acclimation condition. PC/PE radioactivity ratio increased both for medium and high acclimation conditions from 0.493 in control embryos to 1.378 and 1.032 respectively. Phospholipid changes could be relevant for changes in membrane features associated with low level exposures to copper, preparing the embryo for a higher resistance to this metal. The increased resistance to copper could also be associated with both an increase in metallothioneins concentration, as registered with HPLC in all the acclimation conditions, and an increase in the copper bound to the third fraction of metallothioneins separated by this method. Our results point out that even very low level exposure to copper results in phospholipid metabolism changes that could be relevant for the acclimation phenomena.
Fil: Fonovich, Teresa Mabel. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: D'eramo, Jose Luis. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We report phospholipid changes in Rhinella arenarum embryos after applying three acclimation protocols to copper between 40 and 420 ng L− 1. The lower and higher acclimation treatments resulted in embryos' enhanced resistance to this metal. Phospholipid remodeling activity, evident through arachidonic acid radioactivity incorporation increase in phosphatidylcholine (PC) and sphingomyelin (SPH) fractions, was registered in embryos acclimated to the intermediate exposure condition. Concomitantly, a decrease in phosphatidic acid fraction (PA) was registered in the higher acclimation condition. PC/PE radioactivity ratio increased both for medium and high acclimation conditions from 0.493 in control embryos to 1.378 and 1.032 respectively. Phospholipid changes could be relevant for changes in membrane features associated with low level exposures to copper, preparing the embryo for a higher resistance to this metal. The increased resistance to copper could also be associated with both an increase in metallothioneins concentration, as registered with HPLC in all the acclimation conditions, and an increase in the copper bound to the third fraction of metallothioneins separated by this method. Our results point out that even very low level exposure to copper results in phospholipid metabolism changes that could be relevant for the acclimation phenomena.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46801
Fonovich, Teresa Mabel; Perez Coll, Cristina Silvia; Fridman, Osvaldo; D'eramo, Jose Luis; Herkovits, Jorge; Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 10-16
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46801
identifier_str_mv Fonovich, Teresa Mabel; Perez Coll, Cristina Silvia; Fridman, Osvaldo; D'eramo, Jose Luis; Herkovits, Jorge; Phospholipid changes in Rhinella arenarum embryos under different acclimation conditions to copper; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 10-16
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2016.06.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045616300722
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269008887283712
score 13.13397

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