Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae

Autores
Hutler Wolkowicz, Ianina Ruth; Herkovits, Jorge; Perez Coll, Cristina Silvia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The acute and chronic toxicity of bisphenol A (BPA) was evaluated on the common South American toad Rhinella arenarum embryos and larvae by means of continuous and pulse exposure treatments. Embryos were treated continuously from early blastula (S.4) up to complete operculum (S.25), during early larval stages and by means of 24 h pulse exposures of BPA in concentrations ranging between 1.25 and 40 mg L−1, in order to evaluate the susceptibility to this compound in different developmental stages. For lethal effects, S.25 was the most sensitive and gastrula was the most resistant to BPA. The Teratogenic Index for neurula, the most sensitive embryonic stage for sublethal effects was 4.7. The main morphological alterations during early stages were: delayed or arrested development, reduced body size, persistent yolk plug, microcephaly, axial/tail flexures, edemas, blisters, waving fin, underdeveloped gills, mouth malformations, and cellular dissociation. BPA caused a remarkable narcotic effect from gill circulation stage (S.20) onwards in all the organisms exposed after 3 h of treatment with 10 mg L−1 BPA. After recovering, the embryos exhibited scarce response to stimuli, erratic or circular swimming, and spasmodic contractions from 5 mg L−1 onwards. Our results highlight the lethal and sublethal effectsof BPA on R. arenarum embryos and larvae, in the last case both at structural and functional levels.
Fil: Hutler Wolkowicz, Ianina Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; Argentina
Fil: Herkovits, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; Argentina
Fil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bisphenol A
Amphibian Embryos
Stage Dependent Toxicity
Teratology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32609
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32609
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network_name_str CONICET Digital (CONICET)
spelling Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvaeHutler Wolkowicz, Ianina RuthHerkovits, JorgePerez Coll, Cristina SilviaBisphenol AAmphibian EmbryosStage Dependent ToxicityTeratologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The acute and chronic toxicity of bisphenol A (BPA) was evaluated on the common South American toad Rhinella arenarum embryos and larvae by means of continuous and pulse exposure treatments. Embryos were treated continuously from early blastula (S.4) up to complete operculum (S.25), during early larval stages and by means of 24 h pulse exposures of BPA in concentrations ranging between 1.25 and 40 mg L−1, in order to evaluate the susceptibility to this compound in different developmental stages. For lethal effects, S.25 was the most sensitive and gastrula was the most resistant to BPA. The Teratogenic Index for neurula, the most sensitive embryonic stage for sublethal effects was 4.7. The main morphological alterations during early stages were: delayed or arrested development, reduced body size, persistent yolk plug, microcephaly, axial/tail flexures, edemas, blisters, waving fin, underdeveloped gills, mouth malformations, and cellular dissociation. BPA caused a remarkable narcotic effect from gill circulation stage (S.20) onwards in all the organisms exposed after 3 h of treatment with 10 mg L−1 BPA. After recovering, the embryos exhibited scarce response to stimuli, erratic or circular swimming, and spasmodic contractions from 5 mg L−1 onwards. Our results highlight the lethal and sublethal effectsof BPA on R. arenarum embryos and larvae, in the last case both at structural and functional levels.Fil: Hutler Wolkowicz, Ianina Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; ArgentinaFil: Herkovits, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; ArgentinaFil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32609Herkovits, Jorge; Hutler Wolkowicz, Ianina Ruth; Perez Coll, Cristina Silvia; Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae; Wiley; Environmental Toxicology; 29; 2; 11-2011; 146-1541520-40811522-7278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20781info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.20781/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:00Zoai:ri.conicet.gov.ar:11336/32609instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:00.887CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
title Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
spellingShingle Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
Hutler Wolkowicz, Ianina Ruth
Bisphenol A
Amphibian Embryos
Stage Dependent Toxicity
Teratology
title_short Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
title_full Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
title_fullStr Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
title_full_unstemmed Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
title_sort Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae
dc.creator.none.fl_str_mv Hutler Wolkowicz, Ianina Ruth
Herkovits, Jorge
Perez Coll, Cristina Silvia
author Hutler Wolkowicz, Ianina Ruth
author_facet Hutler Wolkowicz, Ianina Ruth
Herkovits, Jorge
Perez Coll, Cristina Silvia
author_role author
author2 Herkovits, Jorge
Perez Coll, Cristina Silvia
author2_role author
author
dc.subject.none.fl_str_mv Bisphenol A
Amphibian Embryos
Stage Dependent Toxicity
Teratology
topic Bisphenol A
Amphibian Embryos
Stage Dependent Toxicity
Teratology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The acute and chronic toxicity of bisphenol A (BPA) was evaluated on the common South American toad Rhinella arenarum embryos and larvae by means of continuous and pulse exposure treatments. Embryos were treated continuously from early blastula (S.4) up to complete operculum (S.25), during early larval stages and by means of 24 h pulse exposures of BPA in concentrations ranging between 1.25 and 40 mg L−1, in order to evaluate the susceptibility to this compound in different developmental stages. For lethal effects, S.25 was the most sensitive and gastrula was the most resistant to BPA. The Teratogenic Index for neurula, the most sensitive embryonic stage for sublethal effects was 4.7. The main morphological alterations during early stages were: delayed or arrested development, reduced body size, persistent yolk plug, microcephaly, axial/tail flexures, edemas, blisters, waving fin, underdeveloped gills, mouth malformations, and cellular dissociation. BPA caused a remarkable narcotic effect from gill circulation stage (S.20) onwards in all the organisms exposed after 3 h of treatment with 10 mg L−1 BPA. After recovering, the embryos exhibited scarce response to stimuli, erratic or circular swimming, and spasmodic contractions from 5 mg L−1 onwards. Our results highlight the lethal and sublethal effectsof BPA on R. arenarum embryos and larvae, in the last case both at structural and functional levels.
Fil: Hutler Wolkowicz, Ianina Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; Argentina
Fil: Herkovits, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Fundación Pro Salud y Medio Ambiente. Programa de Seguridad Química; Argentina
Fil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The acute and chronic toxicity of bisphenol A (BPA) was evaluated on the common South American toad Rhinella arenarum embryos and larvae by means of continuous and pulse exposure treatments. Embryos were treated continuously from early blastula (S.4) up to complete operculum (S.25), during early larval stages and by means of 24 h pulse exposures of BPA in concentrations ranging between 1.25 and 40 mg L−1, in order to evaluate the susceptibility to this compound in different developmental stages. For lethal effects, S.25 was the most sensitive and gastrula was the most resistant to BPA. The Teratogenic Index for neurula, the most sensitive embryonic stage for sublethal effects was 4.7. The main morphological alterations during early stages were: delayed or arrested development, reduced body size, persistent yolk plug, microcephaly, axial/tail flexures, edemas, blisters, waving fin, underdeveloped gills, mouth malformations, and cellular dissociation. BPA caused a remarkable narcotic effect from gill circulation stage (S.20) onwards in all the organisms exposed after 3 h of treatment with 10 mg L−1 BPA. After recovering, the embryos exhibited scarce response to stimuli, erratic or circular swimming, and spasmodic contractions from 5 mg L−1 onwards. Our results highlight the lethal and sublethal effectsof BPA on R. arenarum embryos and larvae, in the last case both at structural and functional levels.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32609
Herkovits, Jorge; Hutler Wolkowicz, Ianina Ruth; Perez Coll, Cristina Silvia; Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae; Wiley; Environmental Toxicology; 29; 2; 11-2011; 146-154
1520-4081
1522-7278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32609
identifier_str_mv Herkovits, Jorge; Hutler Wolkowicz, Ianina Ruth; Perez Coll, Cristina Silvia; Stage dependent toxicity of Bisphenol A on Rhinella arenarum (Anura, Bufonidae) embryos and larvae; Wiley; Environmental Toxicology; 29; 2; 11-2011; 146-154
1520-4081
1522-7278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20781
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.20781/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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